Integrated MechanoBioSystems Lab
整合機械生物系統實驗室
Department of Biomedical EngineeringNational Cheng Kung University
T. K. N. Ngo, H.-L. Wu, Chen, C.-H. Kuo*, T.-Y. Tu* (2025, Jan). Studying the role of thrombomodulin-plasminogen interaction in spatial and interfacial invasion of melanoma metastatic progression. International Journal of Biological Macromolecules, 284, 138053 2025. (SCI, 6/95, POLYMER SCIENCE).
T.-Y. Tu, Y.-C. Hsu, C.-E., Yang, Y.-J. Shyong, C.-H. Kuo, Y.-F. Liu, S.-S. Shih, and C.-L. Lin* (2024, Dec). Topography, Fiber Alignment, Mechanical Stiffness, and Cellular Composition Between Ventral and Dorsal Ligamentum Flavum Layers: Insights Into Hypertrophy Pathogenesis. JOR Spine. (Accepted). (SCI, 16/136, ORTHOPEDICS).
M.-H. Wang, Y.-C. Lee, T.-Y. Tu, P.-C. Huang, L.-S. Jang, C.-H. Chen* . Optimization analysis of the particle focusing and separation parameters of a sheathless microfluidic device using standing surface acoustic waves. Physics of Fluids, 36, 11. (SCI, 2/40, PHYSICS, FLUIDS & PLASMAS).
C.-L. Lin, Y.-W. Chen, C.-H. Kuo, T.-Y. Tu, H.-L. Wu, J.-C. Tsai, and Y.-J. Shyong*, “Calcium phosphate complex of recombinant human thrombomodulin promote bone formation in interbody fusion,” Biofabrication, 2024. (SCI 2023 I.F. 8.2, Rank 12/123 (Q1:9.76%) in subject category: ENGINEERING, BIOMEDICAL, Times Cited:0)
J.-Y. Huang, T.-Y. Wong, T.-Y. Tu, M.-J. Tang, H.-H. Lin, Y.-Y. Hsueh*, “Assessment of Tilapia Skin Collagen for Biomedical Research Applications in Comparison with Mammalian Collagen,” Molecules, 29(2), 402, 2024. (SCI 2023 I.F. 4.2, Rank 88/313 (Q2:28.12%) in subject category: BIOCHEMISTRY & MOLECULAR BIOLOGY, Times Cited: 5)
C.-L. Lin, Y.-W. Su, Y.-W. Chen, C.-H. Kuo, T.-Y. Tu, J.-C. Tsai, and Y.-J. Shyong*, “BMSC loaded photo-crosslinked hyaluronic acid/collagen hydrogel incorporating FG4592 for enhanced cell proliferation and nucleus pulposus differentiation,” International Journal of Biological Macromolecules, 132828, 2024. (SCI 2023 I.F. 6.953, Rank 6/95 (Q1:6.32%) in subject category: POLYMER SCIENCE, Times Cited:0)
C.-L. Lin, Y.-L. Li, Y.-W. Chen, C.-H. Kuo, T.-Y. Tu, Y.-F. Liu, J.-C. Tsai, and Y.-J. Shyong*, “Amphiphilic NLC-Gel formulation loaded with Sebacoyl dinalbuphine ester and Nalbuphine for localized postoperative pain management,” International Journal of Pharmaceutics, 124295, 2024. (SCI 2023 I.F. 5.3, Rank 35/354 (Q1:9.89%) in subject category: PHARMACOLOGY & PHARMACY, Times Cited: 0)
Ngo, T. K. N., Yang, S. J., Mao, B. H., Nguyen, T. K. M., Ng, Q. D., Kuo, Y. L., Tsai, J. H., Saw, S. N., and T.-Y. Tu*, “A deep learning-based pipeline for analyzing the influences of interfacial mechanochemical microenvironments on spheroid invasion using differential interference contrast,” Materials Today Bio, vol. 23, 100820, 2023. (SCI 2023 I.F. 8.7, Rank 11/123 (Q1:8.94%) in subject category: ENGINEERING, BIOMEDICAL, Times Cited: 4)
C. B.-X. Huang and T.-Y. Tu*, “Recent advances in vascularized tumor-on-a-chip,” Frontiers in Oncology, 13:1150332, 2023. (SCI 2023 I.F. 3.5, Rank 107/322 (Q2:33.23%) in subject category: ONCOLOGY, Times Cited: 9)
K. N. Ngo Thi, C.-H. Kuo, and T.-Y. Tu*, “Recent advances in microfluidic-based cancer immunotherapy-on-a-chip strategies,” Biomicrofluidics, 17, 011501, 2023. (SCI 2023 I.F. 2.6, Rank 12/40 (Q2:30.00%) in subject category: PHYSICS, FLUIDS & PLASMAS, Times Cited: 9)
B.-H. Mao, K. M. Nguyen Thi, M.-J. Tang, R. D. Kamm, and T.-Y. Tu*, “The interface stiffness and topographic feature dictate interfacial invasiveness of cancer spheroids,” Biofabrication, 15, 015023, 2023. (SCI 2023 I.F. 8.2, Rank 12/123 (Q1:9.76%) in subject category: ENGINEERING, BIOMEDICAL, Times Cited: 11)
C.-R. Yang, C.-T. Liang, S.-C. Tsai, Y.-C. Wu, C.-W. Liu, H.-H. Yang, T.-Y. Tu, Y.-C. Lee, K.-Y. Hsiao, W.-C. Chang*, W.-L. and A Ma*, “A Novel 3D Culture Scaffold to Shorten Development Time for Multicellular Tumor Spheroids,” International Journal of Molecular Sciences, 23, 13962, 2022. (SCI 2022 I.F. 5.6, Rank 66/285 (Q1:23.16%) in subject category: BIOCHEMISTRY & MOLECULAR BIOLOGY, Times Cited: 2)
T.-Y. Tu*, Y.-P. Shen, S.-H. Lim and Y.-K. Wang, “A Facile Method for Generating a Smooth and Tubular Vessel Lumen Using a Viscous Fingering Pattern in a Microfluidic Device,” Frontiers in Bioengineering and Biotechnology, 10:877480, 2022. (SCI 2022 I.F. 5.7, Rank 16/73 (Q1:21.92%) in subject category: MULTIDISCIPLINARY SCIENCES, Times Cited: 5)
F.-C. Liao, Y.-K. Wang, M.-Y. Cheng, and T.-Y. Tu*, “A Preliminary Investigation of Embedding In Vitro HepaRG Spheroids into Recombinant Human Collagen Type I for the Promotion of Liver Differentiation,” Polymers, 14(9), 1923, 2022. (SCI 2022 I.F. 5.0, Rank 16/86 (Q1:18.6%) in subject category: POLYMER SCIENCE, Times Cited: 3)
L.-C. Wang, L.-C. Chang, G.-L. Su, P.-Y. Chang, H.-F. Hsu, C.-L. Lee, J.-R. Li, M.-C. Liao, S. Thangudu, J. Treekoon, C.-C. Yu, H.-S. Sheu*, T.-Y. Tu*, W.-P. Su*, C.-H. Su*, and C.-S. Yeh.*, “Chemical Structure and Shape Enhance MR Imaging-Guided X-ray Therapy Following Marginative Delivery,” ACS Applied Materials & Interfaces, 14(11), 13056-13069, 2022. (SCI 2022 I.F. 9.5, Rank 55/344 (Q1:15.99%) in subject category: MATERIALS SCIENCE, MULTIDISCIPLINARY, Times Cited: 8) (Supplementary Cover)
K.-W. Wu, C.T. Kuo, and T.-Y. Tu*, “A Highly Reproducible Micro U‐Well Array Plate Facilitating High‐Throughput Tumor Spheroid Culture and Drug Assessment,” Global Challenges, 2170021, 2021. (SCI 2021 I.F. 5.135, Rank 18/74 (Q1:24.32%) in subject category: MULTIDISCIPLINARY SCIENCES, Times Cited: 16) (Cover)
H.-C. Chuang, K.-L. Tsai, K.-J. Tsai, T.-Y. Tu, Y.-J. Shyong, I.-M. Jou, C.-C. Hsu, Y.-F. Liu, and C.-L. Lin*, “Oxidative stress mediates age-related hypertrophy of ligamentum flavum by inducing inflammation, fibrosis, and apoptosis through activating Akt and MAPK pathways,” Aging-US, 12(23), 24168-24183, 2020. (SCI 2020 I.F. 5.682, Rank 10/53 (Q1:18.87%) in subject category: GERIATRICS & GERONTOLOGY, Times Cited: 27)
Y.-W. Chen, M.-C. Chen, K.-W. Wu, and T.-Y. Tu*, “A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO2 Laser Drilling,” Biomedicines, 8(10), 427, 2020. (SCI 2020 I.F. 6.081, Rank 33/276 (Q1:11.96%) in subject category: PHARMACOLOGY & PHARMACY, Times Cited: 20)
C.-L. Lin, Y.-T. Kuo, C.-H. Tsa, Y.-J. Shyong, S.-H. Shih, and T.-Y. Tu*, “Development of an In Vitro 3D Model for Investigating Ligamentum Flavum Hypertrophy,” Biological Procedures Online, 22:20, 2020. (SCI 2020 I.F. 3.244, Rank 36/78 (Q2:46.15%) in subject category: BIOCHEMICAL RESEARCH METHODS, Times Cited: 9)
C.-Y. Chiu, Y.-C. Chen, K.-W. Wu, W.-C. Hsu, H.-P. Lin, H.-C. Chang, Y.-C. Lee, Y.-K. Wang, and T.-Y. Tu*, “Simple In-House Fabrication of Microwells for Generating Uniform Hepatic Multicellular Cancer Aggregates and Discovering Novel Therapeutics,” Materials, 12(20), 3308, 2019. (SCI 2019 I.F. 3.057, Rank 132/314 (Q2:42.04%) in subject category: MATERIALS SCIENCE, MULTIDISCIPLINARY, Times Cited: 8)
Y.-C. Chen, W.-T. Chiu*, C. Chang, P.-C. Wu, T.-Y. Tu, H.-P. Lin*, H.-C. Chang*, “Chemo-photothermal effects of doxorubicin/silica-carbon hollow spheres on liver cancer,” RSC Advances, 8(2018), 36775–36784, 2018. (SCI 2018 I.F. 3.049, Rank 68/172 (Q2:39.54%) in subject category: CHEMISTRY, MULTIDISCIPLINARY, Times Cited: 16)
Before 2016
Jing Bai*, T.-Y. Tu*; Choong Kim, Jean Paul Thiery, Roger D. Kamm*, “Identification of drugs as single agents or in combination to prevent carcinoma dissemination in a microfluidic 3D environment,” Oncotarget, 6(34):36603–36614, 2015. (* co-first author)
Jing Bai*, Giulia Adriani*, T.-M. Dang, T.-Y. Tu, H.-X. Leong Penny, S.-C. Won, Roger D Kamm, Jean-Paul Thiery, “Contact-dependent carcinoma aggregate dispersion by M2a macrophages via ICAM-1 and β2 integrin interactions,” Oncotarget, 6(28): 25295–25307, 2015.
Heejin Choi, Dushan N Wadduwage, T.- Y. Tu, Paul Matsudaira, Peter TC So.*, “Three‐dimensional image cytometer based on widefield structured light microscopy and high‐speed remote depth scanning,” Cytometry Part A, 87(1): 49–60, 2015. (2015’s Best Paper Award)
T.-Y. Tu, Z. Wang, J. Bai, W. Sun, W.- K. Peng, Ruby Y.-J. Huang, Jean Paul Thiery, Roger D. Kamm*, “Rapid prototyping of concave microwells for the formation of 3D multicellular cancer aggregates for drug screening,” Advanced Healthcare Materials, 3(4): 609–616, 2014. (Back Cover)
Young K. Park, T.-Y. Tu, S.-H. Lim, Ivan J. M. Clement, Se Y. Yang, Roger D. Kamm*, “In Vitro Microvessel Growth and Remodeling within a Three-Dimensional Microfluidic Environment,” Cellular and Molecular Bioengineering, 7(1), 15-25, 2014.
T.-Y. Tu*, C.-Y. Chen*, D.-S. Jong, Andrew M. Wo*, “An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange,” Sensors and Actuators B-Chemical, 185: 496–503, 2013. (* co-first author)
Amir R Aref, Ruby Y.-J. Huang, Weimiao Yu, K.-N. Chua, W. Sun, T.-Y. Tu, J. Bai, W.-J. Sim, Ioannis K Zervantonakis, Jean Paul Thiery, Roger D Kamm*, “Screening Therapeutic EMT Blocking Agents in a Three-Dimensional Microenvironment,” Integrative Biology, 5(2): 381–389, 2013.
C.-Y. Chen, T.-Y. Tu, D.-S. Jong, Andrew M Wo*, “Ion channel electrophysiology via integrated planar patch-clamp chip with on-demand drug exchange,” Biotechnology and Bioengineering, 108(6): 1395–1403, 2011.
C.-Y. Chen, C.-H. Chen, T.-Y. Tu, C.-M. Lin, Andrew M. Wo*, “Electrical isolation and characteristics of permanent magnet-actuated valves for PDMS microfluidics,” Lab on a Chip, 11(4): 733–737, 2011.
C.-Y. Chen, T.-Y. Tu, C.-H. Chen, D.-S. Jong*, Andrew M Wo*, “Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording,” Lab on a Chip, 9(16): 2370–2380, 2009.
2023
Ngo Thi Kim Ngan, S.-J. Yang, B.-H. Mao, Q.-D. Ng, S. N. Saw* and T.-Y. Tu*, “An Artificial Intelligence Software for Automatically Analyzing the Interfacial Spheroid Invasion in Tumor Microenvironment using Differential Interference Contrast Microscopic Images,” BMES Annual Meeting, October 11-14, 2023, Seattle, Washington, American.
Ngo Thi Kim Ngan and T.-Y. Tu*, “Effect of thrombomodulin and plasminogen interaction on melanoma spheroid invasion,” AACR Annual Meeting, April 14-19, 2023, Orlando, Florida, America.
2022
Y.-C. Tsai, B.-H. Mao, T.-Y. Tu*, “Studying initial metastasis at the matrix interface with different matrix stiffness in microfluidics,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
G.-L. Su and T.-Y. Tu*, “Engineering a tapered vascular structure for studying the invasive pattern of breast tumor spheroids,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
Yosua Septianto Santoso and T.-Y. Tu*, “Feedback System Control Technique for Optimization of Metastatic Cancer Drug Screening,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
Ngo Thi Kim Ngan, H.-L. Wu, C.-H. Kuo*, T.-Y. Tu*, “Investigating the Effect of Thrombomodulin on Melanoma Cancer Metastasis Using Three-Dimensional Tumor Spheroid Model,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
Szejue Yang, Ngo Thi Kim Ngan, T.-Y. Tu*, Saw Shier Nee*, “The effect of different matrix interfacial adhesive strength in promoting the metastatic pattern of tumor spheroids using an encoder-decoder model,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
Y.-R. Chen, C.-H. Kuo, Y.-J. Shyong, C.-L. Lin*, T.-Y. Tu*, “Understanding the Process of Ligamentum Flavum Hypertrophy through the Effects of Aging and Hypoxia in Regulating the Sprouting Angiogenesis,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
W.-Y. Chang, H.-C. Kuo, T.-Y. Tu*, “Developing an open-source bioinstrumentation for microfluidic education,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
T.-C. Lin, Adelaine H.-X.Chong, T.-Y. Tu*, “Repurposing the Commercial 3D Printer into an Automated Liquid Handling System,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
H.-W. Chang and T.-Y. Tu*, “3D Printed Microfluidic Device for On-chip Cell Culture Through Hydrophilic and Hydrophobic Modulation,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
C.-H. Yu, Yangrui Liu, Z.-C. Leng, T.-Y. Tu*, C.-H. Lin*, J.-Y. Huang*, “A Total Solutioning Workflow for Sample Processing and Precise Nuclei Quantification in 3D Tumor Spheroids Using Unsupervised Algorithm,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
B.-H. Mao, Thi Kim Mai Nguyen, T.-Y. Tu*, “The effects of matrix-substratum interfacial adhesion strength on cancer cell invasion and migration in a three-dimensional hydrogel-based multicellular tumor spheroid platform,” 9th World Congress of Biomechanics, July 10-14, 2022, Taiwan.
2021
G.-L. Su and T.-Y. Tu*, “ Engineering a Tapering Vascular Structure for Studying The Invasive Pattern of Breast Tumor Spheroids,” 2021 International Conference on Smart Sensors, October 14-15, 2021, Taiwan. (Honorable Oral Paper Award)
Y.-C. Tsai and T.-Y. Tu*, “Studying initial metastasis at the matrix interface with different matrix stiffness in microfluidics,” 2021 International Conference on Smart Sensors, October 14-15, 2021, Taiwan. (Distinguished Oral Paper Award)
C.-E. Yang and T-Y Tu*, “Investigation of matrix characteristics in promoting myofibroblast formation in LF hypertrophy,” 2021 International Conference on Smart Sensors, October 14-15, 2021, Taiwan.
2020
G.-L. Su, T.-Y. Tu*, “Development of a Hydrogel Interface with Co-cultured HUVECs to Study the Invasion Pattern of Breast MCTS,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
Y.-T. Li, H.-C. Kuo, T.-Y. Tu*, “Integrating rapid fabrication of 3D printed innovative teaching aids into microfluidics course and its impact on student’s creativity and learning motivation,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
C.-E. Yang, Y.-J. Shyong, C.-I. Lin, T.-Y. Tu*, “Investigation of LF cell aggregation in promoting myofibroblast formation in LF hypertrophy,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
F.-C. Liao and T.-Y. Tu*, “Investigation of in vitro hepatic spheroid culture in promotion of liver differentiation,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
Kim Mai Nguyen and T.-Y. Tu*, “Investigation on the effect of matrix adhesion strength in the metastatic pattern of breast multicellular tumor spheroid invasion,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
C.-H. Yu and T.-Y. Tu*, “Quantitative investigation of the 3D spheroid model using a clearing-enhanced imaging approach,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
B.-Y. Chang and T.-Y. Tu*, “Study on the application of microfluidics to tumor cell migration inhibiting drugs,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
Y.-C. Tsai and T.-Y. Tu*, “Studying initial metastasis at the matrix interface with different matrix stiffness in microfluidics,” The 4th Global Conference on Biomedical Engineering & Annual Meeting of TSBME (GCBME 2020), October, 2020, Taipei, Taiwan.
2018
K.-W. Wu, C.-Y. Chiu, T.-Y. Tu*, “Ultra-stable and ultra-fast fabrication of microwells using laser ablation in plane PS for the formation of size-controlled hepatic tumor spheroids,” Micro Total Analysis Systems (MicroTAS) 2018, November, 2018, Kaohsiung, Taiwan.
C.-Y. Chiu, Y.-C. Chen, K.-W. Wu, Y.-K. Wang, T.-Y. Tu*, “Generation of size-controlled in vitro 3D hepatic tumor model for discovery of therapeutic photo-thermal treatment,” World Congress on Biomechanics 2018, July, 2018, Dublin, Ireland.
2017
K.-W. Wu, Z.-T. Huang, C.-Y. Chiu, T.-Y. Tu*, “Fabrication of size-controlled concave microwells for long-term culture of in vitro 3D hepatic tumor model,” 2017生物醫學工程科技研討會暨科技部醫學工程學門成果發表會, November, 2017, Taiwan.
C.-Y. Chiu, Y.-C. Chen, K.-W. Wu, T.-Y. Tu*, “Rapid fabrication of size-controlled microwells for generation of in vitro 3D hepatic tumor model and discovery of therapeutic treatment,” 2017生物醫學工程科技研討會暨科技部醫學工程學門成果發表會, November, 2017, Taiwan.
Before 2013
T.-Y. Tu*, “Microfluidic 3D cancer-type specific platform for EMT blocking agents screening,” Biomedical Engineering Society Meeting (BMES), 2013, Seattle, USA.
T.-Y. Tu*, “A Microfluidic Co-culture System for Epithelial-Mesenchymal Transition Drug Screening,” Biomedical Engineering Society Meeting (BMES), 2012, Atlanta, USA.
T.-Y. Tu*, “Rapid microwell prototyping, generation of 3D cancer spheroids and EMT drug screening,” The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS) , 2011, Seattle, USA.
T.-Y. Tu*, “A Lab-use Microfluidic Planar Patch-clamp System,” The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), 2010, Groningen, Netherlands.
T.-Y. Tu*, “A microfluidic integration of planar patch-clamp chip with rapid solution exchange system and optical detection,” The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, (MicroTAS), 2009, Jeju, Korea.
T.-Y. Tu*, “Fabrication of hourglass-shaped microaperture via two-stage laser pulses and its application,” The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, (MicroTAS), 2008, San Diego, USA.