Nature of science:
Assessing risks associated with scientific research—scientists attempt to assess the risks associated with genetically modified crops or livestock. (4.8)
Understandings
Gel electrophoresis is used to separate proteins or fragments of DNA according to size.
PCR can be used to amplify small amounts of DNA.
DNA profiling involves comparison of DNA.
Genetic modification is carried out by gene transfer between species.
Clones are groups of genetically identical organisms, derived from a single original parent cell.
Many plant species and some animal species have natural methods of cloning.
Animals can be cloned at the embryo stage by breaking up the embryo into more than one group of cells.
Methods have been developed for cloning adult animals using differentiated cells.
Applications and skills:
Application: Use of DNA profiling in paternity and forensic investigations.
Application: Gene transfer to bacteria using plasmids makes use of restriction endonucleases and DNA ligase.
Application: Assessment of the potential risks and benefits associated with genetic modification of crops.
Application: Production of cloned embryos produced by somatic-cell nuclear transfer.
Design of an experiment to assess one factor affecting the rooting of stem-cuttings.*
PCR
PCR virtual lab by learn.genetics
Manipulation techniques by DNAi
Polymerase Chain Reaction by Sumanas Inc.
Polymerase Chain Reaction by McGraw and Hill
Gel Electrophoresis
Paternity Testing by Sumanas Inc.
Read the article on how to propagate plants from stem cuttings. http://content.ces.ncsu.edu/plant-propagation-by-stem-cuttings-instructions-for-the-home-gardener.
Make a list of all the factors which may affect the rooting of the stem cuttings. Decide which factor would make a good IV, which factor would make a good DV and which factors would become CVs,