2.5 Enzymes

Understandings:

• • Enzymes have an active site to which specific substrates bind.

  • Enzyme catalysis involves molecular motion and the collision of substrates with the active site.

  • Temperature, pH and substrate concentration affect the rate of activity of enzymes.

  • Enzymes can be denatured.

  • Immobilized enzymes are widely used in industry.

• • Applications and skills:

  • Application: Methods of production of lactose-free milk and its advantages.

  • Skill: Design of experiments to test the effect of temperature, pH and substrate concentration on the activity of enzymes.

  • Skill: Experimental investigation of a factor affecting enzyme activity. (Practical 3)