Development of Imaging Tools

In addition to GLIM and Golgi-averaging tools described above, we have developed other image-based quantitative tools during our study of the Golgi and cilium. Some examples are listed below.

1. The imaging bead-retained prey (IBRP) assay can rapidly measure the binding affinity between two proteins in vitro (Zhou et al., Plos One, 2013).

2. The cilium to plasma membrane intensity ratio (CPIR) method yields a cilium-localization metric that is ciliary protein-specific but experiment-independent (Madugula et al., J. Cell Sci., 2016).

3. The single-molecule intensity method can quantify the stoichiometry of cellular large-protein complexes, such as the nuclear pore complex (Tie et al., Biochem. Biophys. Res. Commun., 2016).