Golgi quartet

A large field of image revealing an interesting behavior of the Golgi during the cell cycle. Here is a 20X image of HeLa cells stained by GM130 (green) and Hoechst 33342 (red) and acquired from one of our teaching lab microscopes.

I first noticed it during my teaching of the practical of BS2010 Bioimaging. It is striking to me to observe the frequent appearance of four aligned Golgi lumps (boxed) among otherwise randomly distributed patterns. The “Golgi quartet” is a pair of twin Golgi lumps from the two mitotic daughter cells at the late telophase or G1 phase. The twin Golgi is resulted from the re-assembly of the mitotic Golgi fragments. It was previously described by Ellisman lab (Gaietta et al., PNAS, 2006).