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Giantin (green), GM130 (blue), and GalT-mCherry (red) in Golgi ministacks (side views)
Giantin (green), GM130 (blue), and GalT-mCherry (red) in Golgi ministacks (side views)
HeLa cells transiently expressing GalT-mCherry were treated with nocodazole to induce the formation of Golgi ministacks and subsequently immuno-stained for endogenous Golgi proteins giantin and GM130. 15 Golgi ministacks showing side-views (above) were selected, aligned, normalized in size and intensity, and averaged, resulting in the side-averaged composite image of the Golgi ministack (below). Red, GalT-mCherry. Green, giantin. Blue, GM130. Mahajan D, Tie HC, and Lu L. (2023) Visualizing the Cisternal Organization of Golgi Ministacks in HeLa Cells by Side-averaging. Bio-protocol, 13(8):e4658. (Cover Image)
En face-averaged Golgi mini-stack images. Top, from left to the right, disk-shaped images are giantin, GPP130 and GM130. Bottom, composite images; left: red, giantin; green, GM130. Middle: red, giantin; green, GPP130. Right: red, giantin; green, GPP130; blue, GM130.
A super-resolution image of the native Golgi complex, showing the connected Golgi cisternae. Note the rim-localization of giantin (red) and the interior distribution of ST6Gal1 (green).
The ER in a cultured mammalian cell. Note the polygonal network of tubular ER and small patched of ER sheets.
The ER (green) and microtubules (red) in a cultured mammalian cell. The image illustrates the inter-dependent relationship between the ER and microtubules.
Mitotic ER sheets (green) and spindle microtubules (red).
The morphology of the ER (green) and chromatin (red) during a cell cycle. During the interphase, the ER has both sheets and tubules (noted that the nuclear envelope is a a membrane sheet); during mitosis, including prometaphase, metaphase and anaphase, the ER predominantly organizes it as sheets.
Patches of ER membrane sheets and ER tubular and polygonal network (green). Actin (red).
Interphase and mitotic ER (green) and chromatin (red).
The delicate network of the Golgi complex in a mouse primary fibroblast cell. Red, Arl1 (a trans-Golgi small G protein); green, GM130 (a cis-Golgi Golgin). Note the slight position shift between cis and trans-Golgi.
The delicate network of the Golgi complex in a mouse primary fibroblast cell. Red, Arl1 (a trans-Golgi small G protein); green, GM130 (a cis-Golgi Golgin). Note the slight position shift between cis and trans-Golgi.
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