Publications
Selected Papers
The Atomwise AIMS Program. "AI is a viable alternative to high throughput screening: a 318-target study"
(Kim, H.; Jung, H.; Kee, J.-M.)
Sci Rep 2024, 14, 7526. [Full Text Link]
Kim, H.; Jung, H.; Kim, S.; Seo, J. K..*; Kee, J.-M.* "Identification of a Target Site for Covalent Inhibition of Protein Phosphohistidine Phosphatase 1"
(equal contributions)
ACS Med. Chem. Lett. 2022, 13, 1911–1915. [Full Text Link]
Lee, D.; Lee, Y.; Shin, S. H.; Choi, S. M.; Lee, S. H.; Jeong, S.; Jang, S.*; Kee, J.-M.* "A Simple Protein Histidine Kinase Activity Assay for High-throughput Inhibitor Screening"
(equal contributions)
Bioorg. Chem. 2023, 130, 106232 [Full Text Link]
Recommended by Faculty Recommendation( 08 Mar 2023) [Link]
Bhaskararao, B.; Rotella, M. E.; Kim, D. Y.; Kee, J.-M.*; Kim, K. S.*; Kozlowski, M. C.* "Ir and NHC Dual Chiral Synergetic Catalysis: Mechanism and Stereoselectivity in γ-Butyrolactone Formation"
J. Am. Chem. Soc 2022, 144, 16171–16183. [Full Text Link]
Ahn, S.; Jung, H.; Kee, J.-M.* “Quest for the Crypto-proteome”
(equal contributions)
ChemBioChem 2021, 22, 319-325. (Invited for "Chemical Translational Biology" special issue) [Full Text Link]
Jung, H.; Choi, Y.; Lee, D.; Seo, J. K.; Kee, J.-M.* “Distinct Reaction Dynamics of Protein Arginine Kinases Revealed by Fluorescent Activity Probes”
Chem. Commun. 2019, 55, 7482-7485. [Full Text Link]
Maiti, S.; Kim, J.; Park, J.-H.; Nam, D.; Lee, J. B.; Kim, Y.-J.; Kee, J.-M.; Seo, J. K.; Myung, K.; Rohde, J.-U.; Choe, W.; Kwon, O.-H.; Hong, S. Y. "Chemoselective Trifluoroethylation Reactions of Quinazolinones and Identification of Photostability"
J. Org. Chem. 2019, 84, 6737-6751. [Full Text Link]
Choi, Y.; Shin, S. H.; Jung. H.; Kwon, O.; Seo, J.-K.*; Kee, J.-M.* "A specific fluorescent probe for protein histidine phosphatase activity"
(equal contributions)
ACS Sensors 2019, 4, 1055-1062. [Full Text Link]
Jung, H.; Shin, S. H.; Kee, J.-M.* "Recent Updates on Protein N-Phosphoramidate Hydrolases"
(equal contributions)
ChemBioChem 2019, 20, 623–633. [Full Text Link]
Kang, H.; Yoo, J.; Sohn, B.-K.; Lee, S.-W,; Lee, H. S.; Ma, W.; Kee, J.-M.; Aksimentiev, A.*; Kim, H.* "Sequence-dependent DNA condensation as a driving force of DNA phase separation"
Nucl. Acids Res. 2018, 46 (18), 9401–9413. [Full Text Link]
Kim, W. G.; Kang, M. E.; Lee, J. B.; Jeon, M. H.; Lee, S.; Lee, J.; Choi, B.; Cal, P. M. S. D.; Kang, S.; Kee, J.-M.; Bernardes, G. J. L.; Rohde, J.-U.; Choe, W.; Hong, S. Y. "Nickel-Catalyzed Azide-Alkyne Cycloaddition to Access 1,5-Disubstituted 1,2,3-Triazoles in Air and Water."
J. Am. Chem. Soc. 2017, 139 (35), 12121–12124. [Full Text Link]
Lee, J. B.; Kang, M. E.; Kim, J.; Lee, C. Y.; Kee, J.-M.; Myung, K.; Park, J.-U.; Hong, S. Y. Direct Diversification of Unmasked Quinazolin-4(3H)-Ones Through Orthogonal Reactivity Modulation.
Chem. Commun. 2017, 53 (75), 10394–10397. [Full Text Link]
Oslund, R. C.; Su, X.; Haugbro, M.; Kee, J.-M.; Esposito, M.; David, Y.; Wang, B.; Ge, E.; Perlman,D. P.; Kang,Y.; Muir, T. W.*; Rabinowitz, J. D.* "Bisphosphoglycerate Mutase Controls Serine Pathway Flux via 3-Phosphoglycerate"
Nature. Chem. Biol. 2017, 13, 1081–1087. [Full Text Link]
Kee, J.-M.; Oslund, R. C.; Couvillon, A. D.; Muir, T. W.* "A Second-Generation Phosphohistidine Analog for Production of Phosphohistidine Antibodies"
Org. Lett. 2015, 17, 187-189. [Full Text Link]
Wender, P. A.*; Axtman, A. D.; Golden, J. E.; Kee, J. -M.; Sirois, L. E.; Quiroz, R. V.; Stevens, M. C. "Function through Bio-Inspired, Synthesis-Informed Design: Step-Economical Syntheses of Designed Kinase Inhibitors"
Org. Chem. Front. 2014, 1, 1166-1171. [Full Text Link] (Invited article for Max Malacria's 65th birthday)
Oslund, R. C.; Kee, J.-M.; Anthony D. Couvillon, A. D.; Bhatia, V. N.; Perlman, D. H.; Muir, T. W.* "A Phosphohistidine Proteomics Strategy Based on Elucidation of a Unique Gas-phase Phosphopeptide Fragmentation Mechanism" (co-first authors)
J. Am. Chem. Soc. 2014, 136, 12899–12911 . [Full Text Link]
Highlighted in Nat. Methods 2014, 11, 1091 [Link]
Kee, J.-M.; Oslund, R. C.; Perlman, D. H.; Muir, T. W.* “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation”
Nature Chem. Biol., 2013, 9, 416–421. [Full Text Link]
Nominated as one of “Signaling Breakthroughs of Year 2013”, Sci. Signaling 7 (307), eg1
Featured in Nat. Chem. Biol. 2013, 9, 411, Nat. Methods 2013, 10, 604, and Chem. Eng. News, 91(22), June 3, 2013, p 30.
Kee, J.-M.; Muir, T. W.* “Chasing Phosphohistidine, An Elusive Sibling in the Phosphoamino Acid Family”,
ACS Chemical Biololgy 2012, 7, 44-51. [Full Text Link]
Wender, P. A.*; Buschmann, N.; Cardin, N. B.; Jones, L. R.; Kan, C.; Kee, J.-M.; Kowalski, J. A.; Longcore, K. E. “Gateway Synthesis of Daphnane Congeners and their Protein Kinase C Affinities and Cell-growth Activities”
Nature Chemistry 2011, 3, 615-619. [Full Text Link]
Featured in Nature Chem. 2011, 3,575.
Kee, J.-M.; Villani, B.; Carpenter, L. R.; Muir, T. W.* “Development of Stable Phosphohistidine Analogues”
J. Am. Chem. Soc. 2010, 132, 14327-14329. [Full Text Link]
Selected as “News of the Week” in Chem. Eng. News, 88(41), October 11, 2010, p 11. [Link]
Selected by Faculty of 1000 Biology as “Must Read”.
Wender, P. A.*; Kee, J.-M.; Warrington, J. M. “Practical Synthesis of Prostratin, DPP, and their Analogs, Adjuvant Leads against Latent HIV”
Science, 2008, 320, 649-652. [Full Text Link]
Selected as Research Highlights in Nature, 2008, 453, 136 and Nat. Chem. Biol. 2008, 4, 340.
Lee, H.-Y.*; Kim, H. Y.; Kim, B.; Kee, J.-M. “The Stereoselective Dimerization Reaction of Oxidopyrylium Ions”
Synthesis 2007, 2360-2364. [Full Text Link]
Wender, P. A.*; Bi, F. C.; Buschmann, N.; Gosselin, F.; Kan, C.; Kee, J.-M.; Ohmura, H. “Studies on Oxidopyrylium [5+2] Cycloadditions: Towards a General Synthetic Route to the C12-Hydroxy Daphnetoxins”
Org. Lett. 2006, 8, 5373-5376. [Full Text Link]
Patents
Korean Patent 10-2578953-0000 "Method for Fluorescence-based Realtime Sphingolipid Kinase Activity Probes and the Enzyme Assays"; registered on September 12th, 2023.
PCT application PCT/KR2022/012583 "FRET Biosensor for Measuring Arginine Phosphorylation and Uses thereof"; filed on Aug 23rd, 2022.
Korean Patent Application 10-2021-0142878 "A histidine kinase assay method for high-throughput screening of novel antibacterial compounds"; filed on Oct 25th, 2021.
Korean Patent Application 10-2021-0134066 "Method for real-time measurement of protein Arg phosphorylation dynamics in live cells "; filed on Oct 8th, 2021.
PCT application PCT/KR2021/015114 "COMPOSITION AND METHOD FOR MEASURING HISTIDINE KINASE ACTIVITY, AND METHOD FOR SCREENING HISTIDINE KINASE INHIBITORS BY USING THE SAME"; filed on Oct 26th, 2021.
Korean Patent Application 10-2020-0153518 "A histidine kinase assay method for high-throughput screening of novel antibacterial compounds"; filed on November 17th, 2020.
Korean Patent 10-2042182 "Probes for Detecting Phosphohistidine Phosphatase 1 and Manufacturing Method thereof"; registered on Oct 31st, 2019.
U.S. Patent 10,351,581 B2 “Phosphohistidine Mimetics and Antibodies to Same”; issued on July 16th, 2019
U.S. Patent 9,765,154 “Phosphohistidine Analogs”; issued on September 19th, 2017. [Link]
U.S. Patent 9,035,068 “Phosphohistidine Analogs”; issued on May 19th, 2015. [Link]
The technology has been licensed for commercialization (Millipore).
U.S. Patent Application 61/981,569 “Method for Detecting Cancer and Metabolic Disease using Novel Phosphohistidine Antibodies”; provisional application filed on April 18th, 2014.
U.S. Patent 8,536,378 “Prostratin and Structural or Functional Analogs Thereof”; issued on September 17, 2013 [Link]
U.S. Patent 8,067,632 “A Process to Produce Prostratin and Structural or Functional Analogs Thereof”; issued on November 29, 2011 [Link]
The technology is licensed to AIDS Research Alliance (Los Angeles, CA) in February 2010. [Link]
