Selected Papers

  • Kim, H.; Jung, H.; Kim, S.; Seo, J. K..*; Kee, J.-M.* "Identification of a Target Site for Covalent Inhibition of Protein Phosphohistidine Phosphatase 1"

(equal contributions)

ACS Med. Chem. Lett. ASAP [Full Text Link]

  • Lee, D.; Lee, Y.; Shin, S. H.; Choi, S. M.; Lee, S. H.; Jeong, S.; Jang, S.*; Kee, J.-M.* "A Simple Protein Histidine Kinase Activity Assay for High-throughput Inhibitor Screening"

(equal contributions)

Bioorg. Chem. 2023, 130, 106232 [Full Text Link]

  • Bhaskararao, B.; Rotella, M. E.; Kim, D. Y.; Kee, J.-M.*; Kim, K. S.*; Kozlowski, M. C.* "Ir and NHC Dual Chiral Synergetic Catalysis: Mechanism and Stereoselectivity in γ-Butyrolactone Formation"

J. Am. Chem. Soc 2022, 144, 16171–16183. [Full Text Link]

  • Ahn, S.; Jung, H.; Kee, J.-M.*Quest for the Crypto-proteome

(equal contributions)

ChemBioChem 2021, 22, 319-325. (Invited for "Chemical Translational Biology" special issue) [Full Text Link]

  • Jung, H.; Choi, Y.; Lee, D.; Seo, J. K.; Kee, J.-M.* “Distinct Reaction Dynamics of Protein Arginine Kinases Revealed by Fluorescent Activity Probes”

Chem. Commun. 2019, 55, 7482-7485. [Full Text Link]

  • Maiti, S.; Kim, J.; Park, J.-H.; Nam, D.; Lee, J. B.; Kim, Y.-J.; Kee, J.-M.; Seo, J. K.; Myung, K.; Rohde, J.-U.; Choe, W.; Kwon, O.-H.; Hong, S. Y. "Chemoselective Trifluoroethylation Reactions of Quinazolinones and Identification of Photostability"

J. Org. Chem. 2019, 84, 6737-6751. [Full Text Link]

  • Choi, Y.; Shin, S. H.; Jung. H.; Kwon, O.; Seo, J.-K.*; Kee, J.-M.* "A specific fluorescent probe for protein histidine phosphatase activity"

(equal contributions)

ACS Sensors 2019, 4, 1055-1062. [Full Text Link]

  • Jung, H.; Shin, S. H.; Kee, J.-M.* "Recent Updates on Protein N-Phosphoramidate Hydrolases"

(equal contributions)

ChemBioChem 2019, 20, 623–633. [Full Text Link]

  • Kang, H.; Yoo, J.; Sohn, B.-K.; Lee, S.-W,; Lee, H. S.; Ma, W.; Kee, J.-M.; Aksimentiev, A.*; Kim, H.* "Sequence-dependent DNA condensation as a driving force of DNA phase separation"

Nucl. Acids Res. 2018, 46 (18), 9401–9413. [Full Text Link]

  • Kim, W. G.; Kang, M. E.; Lee, J. B.; Jeon, M. H.; Lee, S.; Lee, J.; Choi, B.; Cal, P. M. S. D.; Kang, S.; Kee, J.-M.; Bernardes, G. J. L.; Rohde, J.-U.; Choe, W.; Hong, S. Y. "Nickel-Catalyzed Azide-Alkyne Cycloaddition to Access 1,5-Disubstituted 1,2,3-Triazoles in Air and Water."

J. Am. Chem. Soc. 2017, 139 (35), 12121–12124. [Full Text Link]

  • Lee, J. B.; Kang, M. E.; Kim, J.; Lee, C. Y.; Kee, J.-M.; Myung, K.; Park, J.-U.; Hong, S. Y. Direct Diversification of Unmasked Quinazolin-4(3H)-Ones Through Orthogonal Reactivity Modulation.

Chem. Commun. 2017, 53 (75), 10394–10397. [Full Text Link]

  • Oslund, R. C.; Su, X.; Haugbro, M.; Kee, J.-M.; Esposito, M.; David, Y.; Wang, B.; Ge, E.; Perlman,D. P.; Kang,Y.; Muir, T. W.*; Rabinowitz, J. D.* "Bisphosphoglycerate Mutase Controls Serine Pathway Flux via 3-Phosphoglycerate"

Nature. Chem. Biol. 2017, 13, 1081–1087. [Full Text Link]

  • Kee, J.-M.; Oslund, R. C.; Couvillon, A. D.; Muir, T. W.* "A Second-Generation Phosphohistidine Analog for Production of Phosphohistidine Antibodies"

Org. Lett. 2015, 17, 187-189. [Full Text Link]

  • Wender, P. A.*; Axtman, A. D.; Golden, J. E.; Kee, J. -M.; Sirois, L. E.; Quiroz, R. V.; Stevens, M. C. "Function through Bio-Inspired, Synthesis-Informed Design: Step-Economical Syntheses of Designed Kinase Inhibitors"

Org. Chem. Front. 2014, 1, 1166-1171. [Full Text Link] (Invited article for Max Malacria's 65th birthday)

  • Oslund, R. C.; Kee, J.-M.; Anthony D. Couvillon, A. D.; Bhatia, V. N.; Perlman, D. H.; Muir, T. W.* "A Phosphohistidine Proteomics Strategy Based on Elucidation of a Unique Gas-phase Phosphopeptide Fragmentation Mechanism" (co-first authors)

J. Am. Chem. Soc. 2014, 136, 12899–12911 . [Full Text Link]

      • Highlighted in Nat. Methods 2014, 11, 1091 [Link]
  • Kee, J.-M.; Oslund, R. C.; Perlman, D. H.; Muir, T. W.* “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation”

Nature Chem. Biol., 2013, 9, 416–421. [Full Text Link]

        • Nominated as one of “Signaling Breakthroughs of Year 2013”, Sci. Signaling 7 (307), eg1

        • Featured in Nat. Chem. Biol. 2013, 9, 411, Nat. Methods 2013, 10, 604, and Chem. Eng. News, 91(22), June 3, 2013, p 30.
  • Kee, J.-M.; Muir, T. W.* “Chasing Phosphohistidine, An Elusive Sibling in the Phosphoamino Acid Family”,

ACS Chemical Biololgy 2012, 7, 44-51. [Full Text Link]

  • Wender, P. A.*; Buschmann, N.; Cardin, N. B.; Jones, L. R.; Kan, C.; Kee, J.-M.; Kowalski, J. A.; Longcore, K. E. “Gateway Synthesis of Daphnane Congeners and their Protein Kinase C Affinities and Cell-growth Activities”

Nature Chemistry 2011, 3, 615-619. [Full Text Link]

      • Featured in Nature Chem. 2011, 3,575.
  • Kee, J.-M.; Villani, B.; Carpenter, L. R.; Muir, T. W.* “Development of Stable Phosphohistidine Analogues”

J. Am. Chem. Soc. 2010, 132, 14327-14329. [Full Text Link]

      • Selected as “News of the Week” in Chem. Eng. News, 88(41), October 11, 2010, p 11. [Link]

      • Selected by Faculty of 1000 Biology as “Must Read”.

  • Wender, P. A.*; Kee, J.-M.; Warrington, J. M. “Practical Synthesis of Prostratin, DPP, and their Analogs, Adjuvant Leads against Latent HIV”

Science, 2008, 320, 649-652. [Full Text Link]

      • Selected as Research Highlights in Nature, 2008, 453, 136 and Nat. Chem. Biol. 2008, 4, 340.

    • Lee, H.-Y.*; Kim, H. Y.; Kim, B.; Kee, J.-M. “The Stereoselective Dimerization Reaction of Oxidopyrylium Ions”

Synthesis 2007, 2360-2364. [Full Text Link]

    • Wender, P. A.*; Bi, F. C.; Buschmann, N.; Gosselin, F.; Kan, C.; Kee, J.-M.; Ohmura, H. “Studies on Oxidopyrylium [5+2] Cycloadditions: Towards a General Synthetic Route to the C12-Hydroxy Daphnetoxins”

Org. Lett. 2006, 8, 5373-5376. [Full Text Link]


  • PCT application PCT/KR2022/012583 "FRET Biosensor for Measuring Arginine Phosphorylation and Uses thereof"; filed on Aug 23rd, 2022.

  • Korean Patent Application 10-2021-0142878 "A histidine kinase assay method for high-throughput screening of novel antibacterial compounds"; filed on Oct 25th, 2021.

  • Korean Patent Application 10-2021-0134066 "Method for real-time measurement of protein Arg phosphorylation dynamics in live cells "; filed on Oct 8th, 2021.


  • Korean Patent Application 10-2021-0033188 "Method for Fluorescence-based Realtime Sphingolipid Kinase Activity Probes and the Enzyme Assays"; filed on March 15th, 2021.

  • Korean Patent Application 10-2020-0153518 "A histidine kinase assay method for high-throughput screening of novel antibacterial compounds"; filed on November 17th, 2020.

  • Korean Patent Application 10-2018-0080937 "Probes for Detecting Phosphohistidine Phosphatase 1 and Manufacturing Method thereof"; filed on July 12th, 2018.

  • U.S. Patent 9,765,154 “Phosphohistidine Analogs”; issued on September 19th, 2017. [Link]

  • U.S. Patent 9,035,068 “Phosphohistidine Analogs”; issued on May 19th, 2015. [Link]

    • The technology has been licensed for commercialization (Millipore).

  • U.S. Patent Application 14/58758 “Phosphohistidine Mimetics and Antibodies to Same”; filed on October 2nd, 2014.

  • U.S. Patent Application 61/981,569 “Method for Detecting Cancer and Metabolic Disease using Novel Phosphohistidine Antibodies”; provisional application filed on April 18th, 2014.

  • U.S. Patent 8,536,378 “Prostratin and Structural or Functional Analogs Thereof”; issued on September 17, 2013 [Link]

  • U.S. Patent 8,067,632 “A Process to Produce Prostratin and Structural or Functional Analogs Thereof”; issued on November 29, 2011 [Link]

    • The technology is licensed to AIDS Research Alliance (Los Angeles, CA) in February 2010. [Link]


  • 기정민, "고에너지 인산화아미노산 - 단백질 인산화 연구의 미개척지", 화학세계, 2015년 12월호 (Mini-review in Korean) [Link]

  • 최이건, 기정민 "인산화아르기닌 - 고전적 생체분자의 재조명", 화학세계, 2017년 4월호 (Mini review in Korean) [Link]