Fermentation- Anaerobic fermentation; Aerobic fermentation- [shake flask, SSF- SmF- Bioreactor (New Brunswick Scientific)-Airlift fermenter]; Handling bio-fermenters (10 L, 100L to 2.5 KL capacity)
Microbiology- Enrichment cultures; Screening & isolation; Identification; Maintenance of various aerobic and anaerobic bacterial and fungal cultures; strain improvement,
Purification: Separation, Chromatography (AKTA-Prime/ Avant/Pilot/ Akta Process- Chromaflow/ Axichrom/Hi-scale/ BPG/ XK); Centrifugation (Continuous, batch, vertical); Cross flow filtration (TFF-50 cm2 to 50 m2); Hollow fibers; Filtration; Homogenizer (GEA NIROSOAVI); Microfluidizer (Microfluidics); Single use technology (Millipore/Sartorius) storage bags and reactors.
Protein Biochemistry - Recombinant protein purification & separation (affinity chromatography, gel permeation, anion exchange); Protein-protein interaction; ELISA; Spectroscopic techniques: fluorescence; ITC; Western hybridization; Enzyme characterization.
Molecular Biology and Genetics- Nucleic acid purification and electrophoresis (DNA/RNA), Nucleic Acid modification (digestion, Ligation); Gene cloning & expression in bacteria and yeast; Vector design and cloning; PCR/RT-PCR; Southern hybridization; Whole-Genome library shotgun cloning; Random and directed mutagenesis; Synthetic genes; Photo-cleavage of DNA by metal-complexes; Flow cytometry (FACS).
Cell culturing- Experience in handling non-microbial cells (animal and plant cell cultures). Metal toxicity assays. Cell banking (Master and Working cell banks)
Animal handling- Mouse and Rat model - Experience in Xenobiotic studies related to male reproductive system (Wistar strain).
Fish Model- Probiotic studies with ornamental fishes.
Insect Model-Silkworm- Bombyxmori-Protein and metabolic studies with Silk worm; Gut-Microbial flora and diversity studies. Silverfish (Lepisma) and Cockroach- Gut Microbial flora studies for isolation of cellulolytic-ethanol genic anaerobic bacteria
Crustacean model- Indian Rice Field Crab (Oziotelphusa senex senex) for Xenobiotic studies
Analytical techniques- HPLC (Shimadzu/Agilent) MALS (WYAT); GC(CIC/Shimadzu); PCR; UV Visible IR Spectrophotometer; ELISA; Immuno-bloting; Chromatographic techniques (TLC/Paper/Column/FPLC etc.,) and other necessary analytical techniques (Balances/Calibration instruments etc.), Flow cytometry.
Microscopy: Fluorescence microscope (Nikon and Olympus) -cell observation; Compound Microscopy-Other automated microscopes.
High throughput technology- used in producing enzymes by using Fungi on various substrates; ELISA. Rota vapor
Microarray application- application of microarray based oligosaccharides and antibodies.
Engineering (non-mathematical)-basic simulation and modeling of filtration units for downstream processing & reactors; Process simulation and design
Scientific Writing - Preparation, writing and reviewing; Experience in preparation of proposals for R&D Grants; preparation of Standard operating protocols.
Computer –[PGDCA], Proficient usage of the main operating systems (Windows, MacOs); Usage of the Microsoft Office pack (Word, Excel, PowerPoint, Publisher); Usage of the Adobe Creative Suite (Photoshop, Illustrator, After Effects)
QMS: SOP, BMR, Specifications, Validations, Qualifications, Risk & Gap Analysis, Risk Mitigation, CAPA, CC, OOS., Deviations, QA, QRM, Auditing (Bio-process)
AI: Apply Artificial Intelligence tool in QMS and Scientific works.
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Auditing- Internal auditing experience, Process validation; Analytical quality Assessment, EHS
Bio-Processing- Fermentation Biology- Molecular Biology, Purification of Biomolecules, QBD/DoE
Bio catalytic Technologies
Biotechnology Industry establishment, GMP/cGMP/GLP/GDP/EHS, QMS etc.,
Biomass and Biofuel; Algal fuel
Doctoral Degree-Ph. D, in Applied Biology (Microbiology)
Enzyme & Food based products Production Technology; Animal feed technology; Aqua Feed; Animal probiotics,
Lab to Commercialization process technology development,
Molecular Cloning and Expression, purification of proteins and bio-chemical and structural characterization,
Novel Ideas for commercial products,
Quality Management: Six Sigma/Quality Assurance/Risk management/Technical Support for Marketing the Scientific Instruments and Bio-tech products,
Research: Doctoral Research, Post-doctoral research, R&D/DoE
Safety: EHS Coordination, Safety activities and awareness camps; Identified best EHS member for two years.
Supply Chain Management- Cold (WHO Certified),
Technology Transfer: Pilot scale/Manufacturing Sciences/MSAT
Vaccines: Polysaccharide Conjugated Vaccines (Up & Down Stream process, Conjugation process; Analytical Research), Viral
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Six Sigma Green Belt from MSME Agra, India- June, 2020
Industrial Biotechnology by EdX (Delftx-MOOCS) from TU Delft. Dec 2015
Introduction to Operation Management –I by EdX (IIMBx-MOOCS) from IIM-B, Oct 2015
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2011: Designer Cellulosome – How to Design, The Weizmann Institute of Science, Rehovot, Israel
2007: Training Program on Entrepreneurship development through Medicinal and Aromatic Plants Technologies, conducted by Department of AYUSH & CIMAP, Hyderabad, Govt. of Andhra Pradesh, India
2003: Refresher Course of Indian Academy of Sciences, Bangalore– inCytogenetics- A Practical Approach, Conducted by Cytogenetics Division, Department of Zoology, BHU, Varanasi, U.P., India
2003: National Workshop - Perspectives of Genetics-A Practical Approach, Conducted by Department of Genetics, Osmania University, Hyderabad, Under UGC (SAP)
2002: Training program on “Biodiversity and Conservation of Mangrove ecosystem of East Coast” at Centre for Advanced Study in Marine Biology, Parangipettai, Annamalai University, Tamil Nadu, India