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This method together with HPLC will help you to establish with reasonable confidence some of the more common haemoglobins. The gel electrophoresis patterns are shown here and it's a case of memorising where the haemoglobin locations are on both the alkaline (pH 8.6) and acid (pH 6.8) gels, which are shown in diagrammatic form respectively.
Commonly multiple samples are run together - and on any gel the control may not be marked! Often on HPLC it will be relatively clear what haemoglobin variant it will be - but with a HbD or HbJ it may be useful to refer to the gels to diagnose this.
For practice gel interpretation you may wish to print out the diagram or this page for reference. Other than being aware of the patterns in which different haemoglobins run there isn't much more theory that you need to know beyond the BCSH guidelines.
On the next page there are some cartoon practice gels. Further gels/plots will be available in the 'Practice Questions' section.
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