Grow Your Bioink

Why grow bioink?
Our dye and ink we use nowadays in industry is polluting our environment and difficult to recycle! Regarding to this environmental pollution problem, we came up with an idea to grow ink from a soil bacteria that naturally produces beautiful pigments. These biosynthesized pigments are organic and recyclable. Our ultimate goal is to integrate the bioink into printers, pens, and the dying process in manufacturing industry.




Phases

This exploration is divided into 3 phases:
 Phase Tasks
 1 Grow bacteria that produce pigment and find out the optimal growth conditions and extract the pigments
 2 Integrate into a continuous ink printer
Study the effect of bioink on marine organisms


Materials  & Methods

We purchased 4 differnet colored bacteria from Carolina Biological Supply
1. Pigmented Bacteria Set, Tube Cultures, Living (Item #154745)
  • Micrococcus luteus (yellow)
  • Rhodococcus rhodochrous (pink)
  • Sarcina aurantiaca (orange-yellow)
  • Serratia marcescens D1 (red).
carolina pigmented bacteria set
2. Microcentrifuge (4000 rpm)
3. Incubator 

Reference
Charkoudian LK, Fitzgerald JT, Khosla C, Champlin A (2010) In Living Color: Bacterial Pigments as an Untapped Resource in the Classroom and Beyond. PLoS Biol 8(10): e1000510. doi: 10.1371/journal.pbio.1000510

Haddix P.L. and Werner T. F. (2000) Spectrophotometric Assay of Gene Expression: Serratia marcescens Pigmentation. Bioscene. Volume 26(4). http://papa.indstate.edu/amcbt/volume_26/v26-4p3-13.pdf 

Pigment Formation in L-forms of Serratia marcescens https://goo.gl/Rpxhri 
 
Extraction, Characterization and Application studies of red pigment of halophile Serratia marcescens KH1R KM035849 isolated from Kharaghoda soil. http://www.ijpab.com/form/2014%20Volume%202,%20issue%206/IJPAB-2014-2-6-160-168.pdf
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