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My driving question: How to use paper chromatography?
My project is about the Chromatography. In this case I will separate or isolate the substances and its solutes of different color dyes in a pen .
MATERIALS:
- Large beaker.
- Small beaker filled with water.
- Filter paper, four strips.
- Black markers for testing, four different brands, water-based
- Masking tape, four pieces for labeling.
- Pencil.
- Permanent marker.
PROCEDURES:
- Number the markers to be used for testing the four strips.
- Write the pen number on a piece of masking tape with a permanent marker and ace it at the top of the corresponding filter paper strip. Number the strips from one to four.
- Draw a thick line near the bottom of the filter paper.
- Pour a small amount of water into the large cup. Attach the unmarked end on a pencil by rolling or taping, and then hang the paper strip in the cup with one end immersed in the water and the ink mark just above the water line.
- Allow the water to move up the paper for five minutes and then remove the strip from the water. Wait till it's dry.
- Do the same procedures with strip two, three, and four.
- Examine the chromatography strips obtained from the four pens.
- Discuss the results, and conclusions.
SAFETY PRECAUTIONS:
- Be careful with any toxic gases.
- Do not breathe in the vapors.
- Wear appropriate clothes that protect you from any chemical substance.
- Remember to do not experiment on an inflammable zone.
The science is the seed of tomorrows and the key of the victory. It is the one that will open you any door. Science will lead you to reach every goal or purpose in your life and career. Science has a relation about our living world, its everything that surround us. The technology is part of it, where every time is introduce and develop to the unknown of the future. Science let us discover all the reasons of our existence.
Investigation Part
Based on your research and area of interest, describe the various components of your project.
Purpose or Driving Question (Identify variables and units of measurement)
How to use paper chromatography?
In this activity I will use paper chromatography to separate the substances and the color
dyes of four different pens through four strips of filter paper.
Procedure: List the steps in your experimental plan. A labeled diagram of the setup is essential.
- Number the markers to be used for testing the four strips.
- Write the pen number on a piece of masking tape with a permanent marker and ace it at the top of the corresponding filter paper strip. Number the strips from one to four.
- Draw a thick line near the bottom of the filter paper.
- Pour a small amount of water into the large cup. Attach the unmarked end on a pencil by rolling or taping, and then hang the paper strip in the cup with one end immersed in the water and the ink mark just above the water line.
- Allow the water to move up the paper for five minutes and then remove the strip from the water. Wait till it's dry.
- Do the same procedures with strip two, three, and four.
- Examine the chromatography strips obtained from the four pens.
Hypothesis: Describe what you think will happen and why. You may base your hypothesis on your research or your own observations/experience.
I think a separation of mixtures is going to happen in the experiment, where I will observed the different and diverse substances that the substances of the pens will introduced.
Materials: Identify all the equipment and chemicals you will need to implement your investigation.
-Large beaker.
- Small beaker filled with water.
- Filter paper, four strips.
- Black markers for testing, four different brands, water-based
- Masking tape, four pieces for labeling.
- Pencil.
- Permanent marker.
Safety: Look up the MSDS of every chemical that you intend to use and underline the hazardous chemicals listed in the materials section. Describe specific safety precautions that you will take to ensure your personal safety as well as the safety of others and the environment. Students planning experiments involving environmental sampling or living organisms must specify measures used to ensure safety for themselves as well as the environment/organisms used.
- Be careful with any toxic gases.
- Do not breathe in the vapors.
- Wear appropriate clothes that protect you from any chemical substance.
- Remember to do not experiment on an inflammable zone.
Observations and Data: Draw a tentative data table showing intervals/trials and units of measurement.
Calculations, Formulas, & Graph Layouts: Specify any formulas, sample calculations and approximate layout of graph with labeled axes and units.
R=2(T - T
2 1
____________
W + W
2 1
Research/References: List 5 references that you used to learn about the scientific concepts related to your experiment and to figure out what is likely to happen in your investigation. Please use standard bibliographic format as learned in your English class.
-Wilson, Ian D.; Adlard, Edward R.; Cooke, Michael et al., eds. (2000). Encyclopedia of separation science. San Diego: Academic Press. ISBN 978-0-12-226770-3
-IUPAC Nomenclature for Chromatography IUPAC Recommendations 1993, Pure & Appl. Chem., Vol. 65, No. 4, pp.819–872, 1993.
-Still, W. C.; Kahn, M.; Mitra, A. J.Org.Chem. 1978, 43(14), 2923–2925. doi:10.1021/jo00408a041
-Laurence M. Harwood, Christopher J. Moody (13 June 1989). Experimental organic chemistry: Principles and Practice (Illustrated ed.). Wiley Blackwell. pp. 180–185. ISBN 978-0-632-02017-1.
- Christian B. Anfinsen, John Tileston Edsall, Frederic Middlebrook Richards Advances in Protein Chemistry. Science 1976, 6-7.
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