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Wide field digital holographic microscopy can realize phase contrast imaging at high precision. But wide field digital holography can not achieve real optical sectioning, and phase sensitivity is compromised by coherent diffraction effect. To solve these issues, we introduced off-axis digital holography method into scanning laser confocal microscopy. To achieve high speed acquisition, line scanning strategy was adopted in this work. We first demonstrated this idea using 2D camera and stage scanning, where each line profile optical field was recorded by and reconstructed from an off-axis digital hologram, and then these line fields were sequentially stitched into the intensity image an phase contrast image respectively. Recently we demonstrated the line camera and beam scanning combined can boost the acquisiton rate to video rate. This new imaging modality can perform optical sectioning as traditional confocal microscopy and simultaneously achieve quantitative phase information of the biological and industrial samples.
- C Liu, S Knitter, Z Cong, I Sencan, H Cao, MA Choma, "High-speed Line-field Confocal Holographic Microscope for Quantitative Phase Imaging", Optics Express 24 (9), 9251-9265 (2016).
- C. Liu, S. Marchesini, M. K. Kim, "Quantitative Phase-Contrast Confocal Microscope," Optics Express 22 (15), 17830-17839 (2014).
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