Publication

Gonzalez-Perez, D., Ratcliffe, J., Tan, S. K., Wong, M. C. M., Yee, Y. P., Nyabadza, N., Xu, J., Wong, T. S., Tee, K. L. (2020). Directed evolution for improved total secretory protein production in Escherichia coli. bioRxiv. doi:10.1101/2020.11.03.366773

Johari, Y. B., Jaffe, S. R. P., Scarrott, J. M., Johnson, A. O., Mozzanino, T., Pohle, T. H., Maisuria, S., Bhayat-Cammack, A., Lambiase, G., Brown, A. J., Tee, K. L., Jackson, P. J., Wong., T. S., Dickman., M. J., Sargur, R., James, D. C. (2020). Production of Trimeric SARS‐CoV‐2 Spike Protein by CHO Cells for Serological COVID‐19 Testing. Biotechnology and Bioengineering. doi:10.1002/bit.27615

Tee, K. L., Jackson, P. J., Scarrott, J. M., Jaffe, S. R. P., Johnson, A. O., Johari, Y., Pohle, T. H., Mozzanino, T., Price, J., Grinham, J., Brown, A. J., Nicklin, M. J., James, D. C., Dickman, M. J., Wong, T. S. (2020). Purification of recombinant SARS-CoV-2 spike, its receptor binding domain, and CR3022 mAb for serological assay. bioRxiv. doi:10.1101/2020.07.31.231282

Keita, V. M., Gonzalez-Villanueva, M., Wong, T. S., & Tee, K. L. (2020). Microbial Utilization of Glycerol for Biomanufacturing. In Engineering of Microbial Biosynthetic Pathways (pp. 245-302). Springer Singapore. doi:10.1007/978-981-15-2604-6_16

Wong, T. S., & Tee, K. L. (2020). A Practical Guide to Protein Engineering. Springer International Publishing. doi:10.1007/978-3-030-56898-6

Alessa, A. H. A., Tee, K. L., Gonzalez-Perez, D., Omar Ali, H. E. M., Evans, C. A., Trevaskis, A., Xu, J., Wong, T. S. (2019). Accelerated directed evolution of dye-decolorizing peroxidase using a bacterial extracellular protein secretion system (BENNY). Bioresources and Bioprocessing, 6(1). doi:10.1186/s40643-019-0255-7

Unrean, P., Tee, K. L., & Wong, T. S. (2019). Metabolic pathway analysis for in silico design of efficient autotrophic production of advanced biofuels. Bioresources and Bioprocessing, 6(1). doi:10.1186/s40643-019-0282-4

Gonzalez-Villanueva, M., Galaiya, H., Staniland, P., Staniland, J., Savill, I., Wong, T. S., & Tee, K. L. (2019). Adaptive laboratory evolution of Cupriavidus necator H16 for carbon co-utilization with glycerol. International Journal of Molecular Sciences, 20(22). doi:10.3390/ijms20225737

Jajesniak, P., Tee, K. L., & Wong, T. S. (2019). PTO-QuickStep: a fast and efficient method for cloning random mutagenesis libraries. International Journal of Molecular Sciences, 20(16). doi:10.3390/ijms20163908

Tee, K. L., Xu, J. -H., & Wong, T. S. (2019). Protein engineering for bioreduction of carboxylic acids. Journal of Biotechnology, 303, 53-64. doi:10.1016/j.jbiotec.2019.07.001

Johnson, A. O., Gonzalez-Villanueva, M., Tee, K. L., & Wong, T. S. (2018). An engineered constitutive promoter set with broad activity range for Cupriavidus necator H16. ACS Synthetic Biology, 7(8), 1918-1928. doi:10.1021/acssynbio.8b00136

Tee, K. L., Grinham, J., Othusitse, A. M., Gonzalez-Villanueva, M., Johnson, A. O., & Wong, T. S. (2017). An efficient transformation method for the bioplastic‐producing ‘Knallgas’ bacterium Ralstonia eutropha H16. Biotechnology Journal, 12(11). doi:10.1002/biot.201700081

Johnson, A. O., Gonzalez-Villanueva, M., Wong, L., Steinb¸chel, A., Tee, K. L., Xu, P., & Wong, T. S. (2017). Design and application of genetically-encoded malonyl-CoA biosensors for metabolic engineering of microbial cell factories. Metabolic Engineering, 44, 253-264. doi:10.1016/j.ymben.2017.10.011

Matthews, S., Belcher, J. D., Tee, K. L., Girvan, H. M., McLean, K. J., Rigby, S. E., Levy, C. W., Leys, D., Parker, D. A., Blankley, R. T., Munro, A. W. (2017). Catalytic determinants of alkene production by the cytochrome P450 peroxygenase OleT_JE. The Journal of Biological Chemistry, 292, 5128-5143. doi:10.1074/jbc.M116.762336

Tee, K. L., & Wong, T. S. (2017). Back to Basics: Creating Genetic Diversity. In Directed Enzyme Evolution: Advances and Applications (pp. 201-227). Springer International Publishing. doi:10.1007/978-3-319-50413-1_8

Matthews, S., Tee, K. L., Rattray, N. J., McLean, K. J., Leys, D., Parker, D. A., Blankley, R. T., Munro, A. W. (2017). Production of alkenes and novel secondary products by P450 OleT JE using novel H₂O₂-generating fusion protein systems. FEBS Letters. doi:10.1002/1873-3468.12581

McLean, K. J., Hans, M., Meijrink, B., van Scheppingen, W. B., Vollebregt, A., Tee, K. L., van der Laan, J., Leys, D., Munro, A. W., van den Berg, M. A. (2015). Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum. Proceedings of the National Academy of Sciences, 112(9), 2847-2852. doi:10.1073/pnas.1419028112

McLean, K. J., Luciakova, D., Belcher, J., Tee, K. L., & Munro, A. W. (2015). Biological Diversity of Cytochrome P450 Redox Partner Systems. In Advances in Experimental Medicine and Biology (pp. 299-317). Springer International Publishing. doi:10.1007/978-3-319-16009-2_11

Tee, K. L., & Wong, T. S. (2014). Directed evolution: A powerful algorithm for advancing synthetic biology. In V. Singh (Ed.), Biotechnology Volume 4: Applied Synthetic Biology (Vol. 4, pp. 465-497). USA: Studium Press LLC.

Tee, K. L., & Wong, T. S. (2013). Polishing the craft of genetic diversity creation in directed evolution. Biotechnology Advances, 31(8), 1707-1721. doi:10.1016/j.biotechadv.2013.08.021

Kuper, J., Tee, K. L., Wilmanns, M., Roccatano, D., Schwaneberg, U., & Wong, T. S. (2012). The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 68(9), 1013-1017. doi:10.1107/s1744309112031570

Zhu, L., Tee, K. L., Roccatano, D., Sonmez, B., Ni, Y., Sun, Z. -H., & Schwaneberg, U. (2010). Directed evolution of an antitumor drug (Arginine Deiminase PpADI) for increased activity at physiological pH. ChemBioChem, 11(5), 691-697. doi:10.1002/cbic.200900717

Tee, K. L. (2009). Exploring genetic diversity in enzyme engineering using Sequence Saturation Mutagenesis (SeSaM). New Biotechnology, 25, S123. doi:10.1016/j.nbt.2009.06.421

Tee, K. L., Dmytrenko, O., Otto, K., Schmid, A., & Schwaneberg, U. (2008). A p-nitrothiophenolate screening system for the directed evolution of a two-component epoxygenase (StyAB). Journal of Molecular Catalysis B: Enzymatic, 50(2-4), 121-127. doi:10.1016/j.molcatb.2007.09.008

Tee, K. L., Roccatano, D., Stolte, S., Arning, J., Jastorff, B., & Schwaneberg, U. (n.d.). Ionic liquid effects on the activity of monooxygenase P450 BM-3. Green Chem., 10(1), 117-123. doi:10.1039/b714674d

Seng Wong, T., Roccatano, D., Loakes, D., Tee, K. L., Schenk, A., Hauer, B., & Schwaneberg, U. (2008). Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): A random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR. Biotechnology Journal, 3(1), 74-82. doi:10.1002/biot.200700193

Tee, K. L., & Schwaneberg, U. (2007). Directed evolution of oxygenases: Screening systems, success stories and challenges. Combinatorial Chemistry & High Throughput Screening, 10(3), 197-217. doi:10.2174/138620707780126723

Tee, K. L., & Schwaneberg, U. (2006). A screening system for the directed evolution of epoxygenases: importance of position 184 in P450 BM3 for stereoselective styrene epoxidation. Angewandte Chemie International Edition, 45(32), 5380-5383. doi:10.1002/anie.200600255

Schenk, A., Wong, T. S., Roccatano, D., Hauer, B., & Schwaneberg, U. (2006). Sequence saturation mutagenesis (SeSaM) of a gene. BioSpektrum, 12(3), 277-279.

Wong, T. S., Tee, K. L., Hauer, B., & Schwaneberg, U. (2005). Sequence saturation mutagenesis with tunable mutation frequencies. Analytical Biochemistry, 341(1), 187-189. doi:10.1016/j.ab.2005.03.023

Wong, T. S., Tee, K.L., Hauer, B., Schwaneberg, U. (2004). Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution. Nucleic Acids Research, 32(3), 26e-26. doi:10.1093/nar/gnh028