Publication
Original paper/Review
*corresponding author, # equal contribution
The clade-specific target recognition mechanisms of plant RISCs
*Hiro-oki Iwakawa
Nucleic Acids Research, gkae257, https://doi.org/10.1093/nar/gkae257, Apr 16, 2024
プレスリリースはこちら。
Mechanisms that regulate the production of secondary siRNAs in plants
Yuji Fujimoto, *Hiro-oki Iwakawa
The Journal of Biochemistry, mvad071, https://doi.org/10.1093/jb/mvad071, Sep 26, 2023
The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
Wei Liu, Keisuke Shoji, Masahiro Naganuma, *Yukihide Tomari, *Hiro-oki Iwakawa
Nucleic Acids Research, gkac1135, https://doi.org/10.1093/nar/gkac1135, Dec 7, 2022
Functional specialization of monocot DCL3 and DCL5 proteins through the evolution of the PAZ domain
Shirui Chen, Wei Liu, Masahiro Naganuma, *Yukihide Tomari, *Hiro-oki Iwakawa
Nucleic Acids Research Apr 5, 2022
Life of RISC: Formation, action, and degradation of RNA-induced silencing complex
*Hiro-oki Iwakawa, *Yukihide Tomari
Molecular Cell 82(1) 30-43 Jan, 2022
Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants
#Yuriki Sakurai, #Kyungmin Baeg, Andy Y. W. Lam, Keisuke Shoji, *Yukihide Tomari, *Hiro-oki Iwakawa
Proceedings of the National Academy of Sciences 118(31) e2102889118-e2102889118 Aug 3, 2021
Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants
*Hiro-oki Iwakawa, Andy Y.W. Lam, Akira Mine, Tomoya Fujita, Kaori Kiyokawa, Manabu Yoshikawa, Atsushi Takeda, Shintaro Iwasaki, Yukihide Tomari
Cell Reports 35(13) 109300-109300 Jun 29, 2021
Plant 22-nt siRNAs mediate translational repression and stress adaptation
#Huihui Wu, #Bosheng Li, Hiro-oki Iwakawa, Yajie Pan, Xianli Tang, Qianyan Ling-hu, Yuelin Liu, Shixin Sheng, Li Feng, Hong Zhang, Xinyan, Zhang, Zhonghua Tang, Xinli Xia, Jixian Zhai, *Hongwei Guo
Nature 581 89-93 May, 2020
In vitro RNA-dependent RNA Polymerase Assay Using Arabidopsis RDR6
Kyungmin Baeg, Yukihide Tomari, *Hiro-oki Iwakawa.
Bio-protocol 8(1) Jan, 2018
Silencing messages in a unique way
*Hiro-oki Iwakawa and *Yukihide Tomari.
Nature Plants 3(10) 769-770 Oct, 2017
Biochemical and single-molecule analyses of the RNA silencing suppressing activity of CrPV-1A
Mariko Watanabe, Hiro-oki Iwakawa, Hisashi Tadakuma, *Yukihide Tomari
Nucleic Acids Research 45(18) 10837-10844 Oct, 2017
Requirement for eukaryotic translation initiation factors in cap-independent translation differs between bipartite genomic RNAs of red clover necrotic mosaic virus
Yuri Tajima, Hiro-oki Iwakawa, Kiwamu Hyodo, Masanori Kaido, Kazuyuki Mise, *Tetsuro Okuno
Virology 509 152-158 Sep, 2017
In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
Yukihide Tomari and *Hiro-oki Iwakawa.
Methods in Molecular Biology 1640 55-71 Jun, 2017
The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing
Kyungmin Baeg, *Hiro-oki Iwakawa and *Yukihide Tomari.
Nature Plants 3(4) Article number: 17036 Apr, 2017
The Functions of MicroRNAs: mRNA Decay and Translational Repression.
Hiro-oki Iwakawa, *Yukihide Tomari
Trends in cell biology 25(11) 651-665 Nov, 2015
MicroRNAs Block Assembly of eIF4F Translation Initiation Complex in Drosophila
#Takashi Fukaya, #Hiro-oki Iwakawa, *Yukihide Tomari.
Molecular Cell 56(1) 67-78 Oct, 2014
Molecular insights into microRNA-mediated translational repression in plants.
Hiro-oki Iwakawa and *Yukihide Tomari.
Molecular Cell 52(4) 591-601 Nov, 2013
Arabidopsis ARGONAUTE7 selects miR390 through multiple checkpoints during RISC assembly.
Yayoi Endo, *Hiro-oki Iwakawa and *Yukihide Tomari.
EMBO reports 14(7) 652-658 Jul, 2013
Poly(A)-Binding Protein Facilitates Translation of an Uncapped/Nonpolyadenylated Viral RNA by Binding to the 3 ' Untranslated Region
Hiro-oki Iwakawa, Yuri Tajima, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Yukihide Tomari, Hisaaki Taniguchi and *Tetsuro Okuno.
Journal of Virology 86(15) 7836-7849 Aug, 2012
A long-distance RNA-RNA interaction plays an important role in programmed-1 ribosomal frameshifting in the translation of p88 replicase protein of Red clover necrotic mosaic virus
Yuri Tajima, Hiro-oki Iwakawa, Masanori Kaido, Kazuyuki Mise, *Tetsuro Okuno
Virology 417(1) 169-178 Aug, 2011
Identification of amino acids in auxiliary replicase protein p27 critical for its RNA-binding activity and the assembly of the replicase complex in Red clover necrotic mosaic virus.
Kiwamu Hyodo, Akira Mine, Hiro-oki Iwakawa, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Virology 413(2) 300-309 May, 2011
Template Recognition Mechanisms by Replicase Proteins Differ between Bipartite Positive-Strand Genomic RNAs of a Plant Virus
Hiro-oki Iwakawa, Akira Mine, Kiwamu Hyodo, Mengnan An, Masanori Kaido, Kazuyuki Mise, *Tetsuro Okuno
Journal of Virology 85(1) 497-509 Jan, 2011
A Y-shaped RNA structure in the 3' untranslated region together with the trans-activator and core promoter of Red clover necrotic mosaic virus RNA2 is required for its negative-strand RNA synthesis.
Mengnan An, Hiro-oki Iwakawa, Akira Mine, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Virology 405(1) 100-109 Sep, 2010
Host-dependent roles of the viral 5' untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3' UTR of Red clover necrotic mosaic virus RNA1.
Siriruk Sarawaneeyaruk, Hiro-oki Iwakawa, Hiroyuki Mizumoto, Hiromi Murakami, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Virology 391(1) 107-118 Aug, 2009
A viral noncoding RNA generated by cis-element-mediated protection against 5'->3' RNA decay represses both cap-independent and cap-dependent translation.
Hiro-oki Iwakawa, Hiroyuki Mizumoto, Hideaki Nagano, Yuka Imoto, Kazuma Takigawa, Siriruk Sarawaneeyaruk, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Journal of virology 82(20) 10162-10174 Oct, 2008
cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
Kimiyuki Okamoto, Hideaki Nagano, Hiro-oki Iwakawa, Hiroyuki Mizumoto, Atsushi Takeda, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Virology 375(1) 205-212 May, 2008
cis-Acting core RNA elements required for negative-strand RNA synthesis and cap-independent translation are separated in the 3'-untranslated region of Red clover necrotic mosaic virus RNA1.
Hiro-oki Iwakawa, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Virology 369(1) 168-181 Dec, 2007
Cap-independent translation mechanism of red clover necrotic mosaic virus RNA2 differs from that of RNA1 and is linked to RNA replication.
#Hiroyuki Mizumoto, #Hiro-oki Iwakawa, Masanori Kaido, Kazuyuki Mise and *Tetsuro Okuno.
Journal of virology 80(8) 3781-3791 Apr, 2006
日本語総説など
植物の二次的小分子RNAの生成機構
藤本 祐司，岩川 弘宙
Journal of Japanese Biochemical Society 95(3): 346-350 (2023)
自然免疫における環状RNAの役割
岩川 弘宙
実験医学 38(3) 436-437 2020年2月
植物のRNAサイレンシング機構
岩川 弘宙
植物感染生理談話会論文集 (53) 46-56 2018年8月
植物のRNAサイレンシング機構が自己のmRNAを攻撃しないメカニズム
岩川 弘宙
植物科学最前線 8 58-70 2017年
植物と動物におけるmicroRNAによる翻訳制御機構
岩川 弘宙, 泊 幸秀
化学と生物 53(8) 510-514 2015年
miRNAによる翻訳開始因子複合体の形成の阻害
深谷雄志, 岩川 弘宙, 泊 幸秀
ライフサイエンス新着論文レビュー 2014年
植物におけるマイクロRNAを介した翻訳抑制機構
岩川 弘宙, 泊 幸秀
ライフサイエンス新着論文レビュー 2013年
RNAサイレンシングのしくみ
岩川 弘宙, 泊幸秀
週刊「医学のあゆみ」 238(5) 388-393 2011年
ウイルスが生み出す様々なノンコーディング RNA
岩川 弘宙, 奥野哲郎
59(2) 179-188 2009年