In this Spring 2022 half-semester course, I worked on a process development proposal for generating recombinant proteins in Pichia pastoris. In this paper, I highlight current research on recombinant protein production methods using P. pastoris. I also describe limitations associated with current protein production using P. pastoris and propose a new process to potentially overcome these limitations.
I also worked on a presentation about recombinant protein production in plants. In this presentation, my lab partner, Connor Steenbock, and I discuss how plant cell fermentation has been used to create various recombinant protein products including biotherapeutics, cell-free meat alternatives, and polyhydroxyalkanoates (PHAs). We also discuss the advantages and disadvantages of plant cell fermentation compared to those of bacterial and mammalian cells.
In September 2019, I was accepted into an honors thesis program known as the George H. Cook Scholars Program. My research was done in the biodegradation of pharmaceuticals and personal care products. More specifically, I studied anaerobic biodegradation of acetylsalicylic acid, which is more commonly known as aspirin, in aquatic sediments. I used anaerobic culturing techniques to isolate and enrich degradative cultures sampled from three sites: the Hudson River, the Raritan River, and a Raritan wastewater treatment plant. I also utilized various analytical techniques to study the overall biodegradation mechanism including High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry (GC-MS).
MB 590: Microbial Symbiosis and Microbiomes
MB 714: Microbial Metabolic Regulation
BIT 510: Methods in Recombinant DNA
PP 501: Biology of Plant Pathogens
PB 580: Introduction to Plant Biotechnology
BEC 563: Fermentation of Recombinant Microorganisms
BEC 577: Advanced Biomanufacturing and Biocatalysis
Biotechnology Courses: Molecular Genetics, Nucleotide Sequence Analysis, Tools for Bioinformatic Analysis, Methods and Applications in Molecular Biology, Seminar in Biotechnology
Microbiology Courses: General Microbiology, Applied Microbiology, Microbial Genetics and Genomics, Environmental Microbiology
Biochemistry Courses: Introduction to Biochemistry, General Biochemistry, Introductory Biochemistry Lab
Chemistry Courses: General Chemistry, Organic Chemistry, Introduction to Experimentation
Other: Genetics