Welcome to Introduction to BLAST Searching! This video series will cover the basics of what BLAST is, and how to run a BLAST search and interpret the results. For more in depth help or a one on one consultation, feel free to contact aglifesci_librarians@ncsu.edu
This video introduces the NCBI BLAST search and defines the key terms for setting up searches and interpreting their results. Use the link below to access the presentation slides.
This video is a demo of running a NCBI Nucleotide BLAST (blastn) search and interpreting its results. The sequences used in this demo are linked through the button below, feel free to try the search out on your own!
This video is a demo of running a NCBI Protein BLAST (blastp) search and interpreting its results. The sequences used in this demo are linked through the button below, feel free to try the search out on your own!
FIRST use the button above to access the practice exercises and try to solve them on your own! This video walks through the solutions to the exercise questions.
You can work directly with a librarian to answer your questions about the NCBI suite of resources. Contact aglifesci_librarians@ncsu.edu
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2.
Rein, D. (2015, October). Fundamentals in Bioinformatics and Searching. National Library of Medicine (US), National Center for Biotechnology Information. Retrieved from https://www.ncbi.nlm.nih.gov/education/librarian/.
Rein, D. (2016, March). A Librarian’s Guide to NCBI. National Library of Medicine (US), National Center for Biotechnology Information. Retrieved from https://www.ncbi.nlm.nih.gov/education/librarian/.
The NCBI Handbook. (2013) (2nd ed.). National Library of Medicine (US), National Center for Biotechnology Information. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK143764/.
Tutorials – Learn – NCBI. National Library of Medicine (US), National Center for Biotechnology Information. Retrieved from https://www.ncbi.nlm.nih.gov/home/tutorials/.