4a: Effect of temperature on enzyme activity (catalase and hydrogen peroxide) [2016]***

Catalase speeds up the decomposition of hydrogen peroxide.

H₂O₂ → 2 H₂O + O₂

Samples that contain catalase: Raw chicken meat, potato, liver

a. Liver Chopped into pieces with hydrogen peroxide

Results

(from left to right)

Boiled liver, Chopped liver, liver, chicken, potato

Boiled liver - catalase are denatured

b. Uncooked (contain active catalase) and cooked potato (contain denatured catalase) with hydrogen peroxide

Effervescence / bubbles (oxygen) are seen in the test-tube that contain uncooked potato.

Uncooked potato cubes Cooked potato cubes

c. Release of oxygen (decomposition of hydrogen peroxide)

Positive oxygen test: Relights a glowing splint