Results

Engineering Phage to target PPEA

Successful editing of the phage allowed for the binding site at the LPS to be altered and bound to an amine

The effects of this change are

Image despcription: phage with red 'feet' matched to a red binding sight on the LPS structure with an arrow pointing to a phage with green 'feet' matched to a green binding site on the amine attatched to the LPS structure

The images above are screenshots from the Benchling software that was used to design the primers that would be tranformed into the plasmids, the HI loop (forward and reverse) are shown in red and green on both images.

Gel Electrophoresis of Engineered Phage Library

after PCR and cloning the HI loop plasmids were imaged via gel electrophoresis to make sure that their size was what we wanted in order to infect the bacteria

image description: black background with white spots indicating phage DNA length compared to a ruler for measurement purposes

Transformation Yield

this graph shows the CFU per microgram of the transformed bacterial library plaqued on kan and a carb control plate, the tranformation of the HI tail fiber loop was successful

image description: two blue bars indicating bacterial density after transformation, the control bar reaches above 1 billion units per microgram and the transformed bacterial library reaches slightly less but still more than 1 million

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