Method

Design degenerate primers using Benchling software

Mutate HI loop of T3 Phage tail fibers using the primers and LPS

Perform PCR then clone plasmids

Grow bacteria with the plasmids, the edited tail fiber will be included into phage genome through homologous recombination with the plasmids

Use bacteria with Colistin resistance to screen phage; Selecting for phage mutants that have expanded host range to infect the strain

Characterize further to determine ability to restore Colistin sensitivity