Experts

Julia Uitz

• Laboratoire d’Océanographie de Villefranche, France
• julia.uitz@obs-vlfr.fr

Sandy Thomalla

• Southern Ocean Carbon & Climate Observatory, South Africa
• sthomalla@csir.co.za

Hervé Claustre

• Laboratoire d’Océanographie de Villefranche, France
• claustre@obs-vlfr.fr

Anya Waite

• Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Germany
• anya.waite@awi.de

Marcela Cornejo D’Ottone

• Instituto Milenio de Oceanografía Barrio Universitario Concepción, Chile
• marcela.cornejo@ucv.cl

Outside of the WG:

Ivona Cetinić

• NASA Goddard Space Flight Center, Greenbelt , Maryland, USA
• ivona.cetinic@nasa.gov

Lucile Duforêt

• Laboratoire d'Océanologie et de Géosciences, France
• lucile.duforet@univ-littoral.fr

Joséphine Ras

• Laboratoire d’Océanographie de Villefranche, France
• jras@obs-vlfr.fr

Lesley Clemenston

• CSIRO Oceans and Atmosphere, Hobart, Tasmania, Australia
• Lesley.Clementson@csiro.au

HPLC

Articles and Book Chapters

Aiken J., Pradhan Y., Barlow R., Lavender S., Poulton A., Holligan P., Hard-Mountford N., 2009. Phytoplankton pigments and functional types in the Atlantic Ocean: A decadal assessment, 1995-2005. Deep-Sea Research II, 56, 899-917.

Barlow R. G., Aiken J., Moore G. F., Holligan P. M., Lavender S., 2004. Pigment adaptations in surface phytoplankton along the eastern boundary of the Atlantic Ocean. Marine Ecology Progress Series, 281, 13-26.

Brewin R. J. W., Sathyendranath S., Lange P. K., Tilstone, G. H., 2014. Comparison of two methods to derive the size-structure of natural populations of phytoplankton. Deep-Sea Research Part I, 85, 72-79, doi:10.1016/j.dsr.2013.11.007.

Claustre H., 1994. The trophic status of various oceanic provinces as revealed by phytoplankton pigment signatures. Limnology and Oceanography, 39, 5, 1206-1210.

Gieskes W. W. C., Kraay G. W., 1983. Dominance of Cryptophyceae during the phytoplankton spring bloom in the central North Sea detected by HPLC analysis of pigments. Marine Biology, 75, 2-3, 179-185.

Jeffrey S. W., R. F. C. Mantoura and S. W. Wright, 1997. Phytoplankton pigments in oceanography. UNESCO, Paris, pp. 447.

Mackey M. D., Mackey D. J., Higgins H. W., S. W. Wright, 1996. CHEMTAX: A program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Marine Ecology Progress Series, 144, 1-3, 265-283.

Peloquin J., C. Swan, N. Gruber, et al., 2013. The MAREDAT global database of high performance liquid chromatography marine pigment measurements. Earth System Scientific Data, 5, 109-123, doi:10.5194/essd-5-109-2013, 2013.

Roy S., C. Llewellyn, E.S. Egeland, G. Johnsen, 2011. Phytoplankton pigments: Characterization, chemo-taxonomy and applications in oceanography, Cambridge University Press, Cambridge, UK.

Uitz J., Claustre H., Morel A., Hooker S.B. (2006). Vertical distribution of phytoplankton communities in open ocean: an assessment based on surface chlorophyll. Journal of Geophysical Research-Oceans, 111, C08005, 1-23, doi:10.1029/2005JC003207.

Vidussi F., Claustre H., Manca B. B., Luchetta A., Marty J. C., 2001. Phytoplankton pigment distribution in relation to upper thermocline circulation in the eastern Mediterranean Sea during Winter. Journal of Geophysical Research-Oceans, 106, C9, 19,939-19,956.

Protocols

Bidigare R. R., L. Van Heukelem and C. C. Trees, 2002. HPLC phytoplankton pigments: sampling, laboratory methods, and quality assurance procedures. In: J L Mueller and G S Fargion (Eds), Ocean optics protocols for satellite ocean color sensor validation. NASA Goddard Space Flight Center, Greenbelt, MD, pp. 258-268.

Garrido J.L., Airs R.L., Rodriguez F., van Heukelem L., and Zapata M., 2011. New HPLC separation techniques. In: Roy S., Llewellyn C., Egeland E., Johnsen G. (Eds.) Phytoplankton pigments: characterization, chemotaxonomy and applications in oceanography. Cambridge University Press, Cambridge, pp. 165-194.

Ras J., H. Claustre, and J. Uitz, 2008. Spatial variability of phytoplankton pigment distributions in the subtropical South Pacific Ocean: Comparison between in situ and modelled data. Biogeosciences, 5, 353-369.

Sanz N., García-Blanco A., Gavalás-Olea A., Loures P., and Garrido J. L., 2015. Phytoplankton pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column. Methods in Ecology and Evolution, 6, 1199–1209. doi:10.1111/2041-210X.12406.

Van Heukelem L. and C. S. Thomas, 2001. Computer-assisted high- performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments. Journal of Chromatography A, 910, 31–49.

Van Heukelem L., Hooker, S.B. 2011. The importance of a quality assurance plan for method validation and minimizing uncertainties in the HPLC analysis of phytoplankton pigments. In: Roy, S., Llewellyn, C., Egeland, E., Johnsen, G. (Eds.) Phytoplankton pigments: characterization, chemotaxonomy and applications in oceanography. Cambridge University Press, Cambridge, pp. 195-242.

Zapata M., Rodríguez. F, Garrido J.L., 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Marine Ecology Progress Series, 195, 29–45.

Inter-comparison

Hooker S.B., H. Claustre, J. Ras, L. Van Heukelem, J-F. Berthon, C. Targa, D. van der Linde, R. Barlow, and H. Sessions, 2000. The First SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-1). NASA Tech. Memo. 2000–206892, Vol. 14, S.B. Hooker and E.R. Firestone, Eds., NASA Goddard Space Flight Center, Greenbelt, Maryland, 42 pp.

Claustre H., Hooker S., Van Heukelem L., Berthon J.-F., Barlow R., Ras J., Sessions H., Targa C., Thomas C.S., Van der Linde D., Marty J.-C., 2004. An intercomparison of HPLC phytoplankton pigment methods using in situ samples: application to remote sensing and database activities. Marine Chemistry, 85, 41-61.

Hooker S.B., L. Van Heukelem, C.S. Thomas, H. Claustre, J. Ras, L. Schlüter, J. Perl, C. Trees, V. Stuart, E. Head, R. Barlow, H. Sessions, L. Clementson, J. Fishwick, C. Llewellyn, and J. Aiken, 2005. The Second SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-2). NASA Tech. Memo. 2005–212785, NASA Goddard Space Flight Center, Greenbelt, Maryland, 112 pp.

Hooker S.B., L. Van Heukelem, C.S. Thomas, H. Claustre, J. Ras, L. Schlüter, L. Clementson, D. van der Linde, E. Eker-Develi, J-F. Berthon, R. Barlow, H. Sessions, H. Ismail, and J. Perl, 2009. The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3). NASA Tech. Memo. 2009-215849, NASA Goddard Space Flight Center, Greenbelt, Maryland, 97 pp.

Hooker S.B., C.S. Thomas, L. Van Heukelem, L. Schlüter, M.E. Russ, J. Ras, H. Claustre, L. Clementson, E. Canuti, J-F. Berthon, J. Perl, C. Normandeau, J. Cullen, M. Kienast, and J. Pinckney, 2010. The Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4). NASA Tech. Memo. 2010-215857, NASA Goddard Space Flight Center, Greenbelt, MD, 74 pp.

Hooker S. B., L. Clementson, C.S. Thomas, L. Schlüter, M. Allerup, J. Ras, H. Claustre, C. Normandeau, J. Cullen, M. Kienast, W. Kozlowski, M. Vernet, S. Chakraborty, S. Lohrenz, M. Tuel, D. Redalje, P. Cartaxana, C.R. Mendes, V. Brotas, S.G.P Matondkar, S.G. Parab, A. Neeley, and E.S. Egeland, 2012. The Fifth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-5), NASA Tech. Memo. 2012- 217503, NASA Goddard Space Flight Center, Greenbelt, MD, 108 pp.

Van Heukelem, and C.S. Thomas, and P.M. Glibert, 2002. Sources of Variability in Chlorophyll Analysis by Fluorometry and High-Performance Liquid Chromatography in a SIMBIOS Inter-calibration Exercise. NASA Tech. Memo. 2002–211606, G.S. Fargion and C.R. McClain, Eds., NASA Goddard Space Flight Center, Greenbelt, Maryland, 50 pp.

POC

Articles

Duforet-Gaurier L., Loisel L., Dessailly D., Nordkvist K., Alvain S., 2010. Estimates of particulate organic carbon over the euphotic depth from in situ measurements, Application to satellite data over the global ocean. Deep-Sea Research I, 57, 351-367, doi.org/10.1016/j.dsr.2009.12.007.

Gardner W., Mishonov A., Richardson M. J., 2006. Global POC concentrations from in situ and satellite data, Deep Sea Research II, 53, 718-740, doi: 10.1016/j.dsr2.2006.01.029.

Jackson T., Sathyendranath S., Platt T., 2017. An exact solution for modeling photoacclimation of the carbon-to-chlorophyll ratio in phytoplankton. Frontiers in Marine Science, 4, 283, doi:10.3389/fmars.2017.00283.

Loisel H., Nicolas J.-M., Deschamps P.-Y., Frouin R., 2002. Seasonal and interannual variability of particulate organic carbon matter in the global ocean. Geophysical Research Letter, 29, 24, doi:10.1029/2002GL015948.

Rasse R., Dall'Olmo G., Graff J., Westberry T. K., van Dongen-Vogels V., Behrenfeld M. J., 2017. Evaluating Optical Proxies of Particulate Organic Carbon across the Surface Atlantic Ocean. Frontiers in Marine Science, 4, 367, doi:10.3389/fmars.2017.00367.

Stramski D., R.A. Reynolds, M. Babin, S. Kaczmarek, M.R. Lewis, R. Röttgers, A. Sciandra, M. Stramska, M.S. Twardowski, B.A. Franz, and H. Claustre, 2008. Relationships between the surface concentration of particulate organic carbon and optical properties in the eastern South Pacific and eastern Atlantic Oceans. Biogeosciences, 5, 171–201.

Protocols

Claustre H., Morel A., Babin M., Caillau C, Marie D., Marty J.-C., Vaulot D., 1999. Variability in particle attenuation and stimulated fluorescence in the tropical and equatorial Pacific: Scales, patterns and some biogeochemical implications. Journal of Geophysical Research-Oceans, 104, 3401–3422.

Cetinić I., Perry M. J., Briggs N. T., Kallin E., D’Asaro E. A., Lee C. M., 2012. Particulate organic carbon and inherent optical properties during 2008 North Atlantic Bloom Experiment, Journal of Geophysical Research-Oceans, doi:10.1029/2011JC007771.

Gardner W. D., Richardson M. J., Carlson C. A., Hansell D., Mishonov A. V. , 2003. Determining true particulate organic carbon: Bottles, pumps and methodologies. Deep-Sea Research Part II, doi:10.1016/S0967-0645(02)00589-1.

Knap A., Michaels A., Close A., Ducklow H., Dickson A., 1996. Protocols for the Joint Global Ocean Flux studies (JGOFS) core measurements, JGOFS Rep. 19, JGOFS Core Proj. Off., Bergen, Norway. Reprint of Intergovernmental Oceanographic Commission Manuals and Guides, no. 29, 170 pp., UNESCO, Paris, 1994.

Stramski D., Reynolds R. A., Babin M., Kaczmarek S., Lewis M. R., Rottgers R., Claustre H., 2008. Relationships between the surface concentration of particulate organic carbon and optical properties in the eastern South Pacific and eastern Atlantic Oceans. Biogeosciences, 5, 171-201, doi:10.5194/bg-5-171-2008.

Morán X., Gasol J., Arin L., Estrada M., 1999. A comparison between glass fiber and membrane filters for the estimation of phytoplankton POC and DOC production. Marine Ecology Progress Series, 187, 31-41.

Raimbault P., Diaz F., Pouvesle W., Boudjellal B., 1999. Simultaneous determination of particulate organic carbon, nitrogen and phosphorus collected on filters, using a semi-automatic wet-oxidation method. Marine Ecology Progress Series, 180, 289-295.

Inter-comparison

King P, Kennedy H, Newton PP, Jickells TD, Brand T, Calvert S, Cauwet G, Etcheber H, Head B, Khripounoff A, Manighetti B, Miquel JC (1998). Analysis of total and organic carbon and total nitrogen in settling oceanic particles and a marine sediment: an interlaboratory comparison. Marine Chemistry, 60(3-4), 203-216, doi:10.1016/S0304-4203(97)00106-0.

HPLC and POC Report

Connections to Existing Programs and Modeling

Instrument Analysis Summary