Paired-agent fluorescence guided surgery in head and neck cancer (NIH R37 funded from 2018-2025)—my lab supports data analysis in this clinically directed work led by Prof. Kimberley Samkoe at Dartmouth to use paired-agent imaging to guide primary head and neck cancer resection. Publications: [9-12].
MRI-guided fluorescence tomography to quantify drug efficacy in preclinical models (NIH R01 funded from 2014-2020). Lead PI is Scott Davis at Dartmouth. Publications: [13-19].
Intracellular quantitative fluorescence molecular imaging. Our lab provides imaging protocol development and data analysis support in a team collaboration between Kimberley Samkoe at Dartmouth (and her expertise in cancer models) and Summer Gibbs at OHSU (and her expertise in developing cell permeable fluorescent imaging agents). Mark Foundation for Cancer Research ASPIRE Award, 2018-2021.
Ultrasound molecular imaging for in vivo detection of cancer-positive lymph nodes (Currently, Geoff Luke at Dartmouth and I are multi-PIs on a submitted grant). The idea is to use vibrational properties of different microbubbles in an ultrasound scan to carry out paired-agent imaging in vivo at depths unreachable by fluorescence. This work is founded our work demonstrating that as few as 200 cancer cells could be detected in a wide-field paired-agent fluorescence imaging [20].
Molecular and mechanical property imaging to elucidate why cancer drugs fail. A collaboration I am leading with Tom Irving at the Advanced Photon Source at Argonne National Laboratories and Illinois Tech to use x-ray diffraction, elastography, and fluorescence molecular imaging to evaluate conditions when cancer drugs work and do not work in pancreatic cancer models (early work is being funded by an Illinois Tech institutional Research grant).
Low dose phase-contrast CT for mammography. (Funded by an NIH R01 led by my collaborator Jovan Brankov in ECE at Illinois Tech, 2018-2022—our lab runs the cancer animal models)
REFERENCES
[1] L. Sinha, F. Massanes, V. C. Torres, C. Li, K. M. Tichauer, and J. G. Brankov, "Comparison of time- and angular-domain scatter rejection in mesoscopic optical projection tomography: a simulation study," (in eng), Biomed Opt Express, vol. 10, no. 2, pp. 747-760, Feb 2019, doi: 10.1364/BOE.10.000747.
[2] V. C. Torres et al., "Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes," (in eng), J Biomed Opt, vol. 24, no. 11, pp. 1-4, 11 2019, doi: 10.1117/1.JBO.24.11.110501.
[3] L. Sinha, M. Fogarty, W. Zhou, A. Giudice, J. G. Brankov, and K. M. Tichauer, "Design and characterization of a dead-time regime enhanced early photon projection imaging system," (in eng), Rev Sci Instrum, vol. 89, no. 4, p. 043707, Apr 2018, doi: 10.1063/1.5003620.
[4] L. Sinha, J. G. Brankov, and K. M. Tichauer, "Enhanced detection of early photons in time-domain optical imaging by running in the "dead-time" regime," (in eng), Opt Lett, vol. 41, no. 14, pp. 3225-8, Jul 2016, doi: 10.1364/OL.41.003225.
[5] S. Kang, X. Xu, E. Navarro, Y. Wang, J. T. C. Liu, and K. M. Tichauer, "Modeling the binding and diffusion of receptor-targeted nanoparticles topically applied on fresh tissue specimens," (in eng), Phys Med Biol, vol. 64, no. 4, p. 045013, Feb 2019, doi: 10.1088/1361-6560/aaff81.
[6] S. Kang, Y. W. Wang, X. Xu, E. Navarro, K. M. Tichauer, and J. T. C. Liu, "Microscopic investigation of" topically applied nanoparticles for molecular imaging of fresh tissue surfaces," (in eng), J Biophotonics, vol. 11, no. 4, p. e201700246, Apr 2018, doi: 10.1002/jbio.201700246.
[7] X. Xu, Y. Wang, J. Xiang, J. T. C. Liu, and K. M. Tichauer, "Rinsing paired-agent model (RPAM) to quantify cell-surface receptor concentrations in topical staining applications of thick tissues," (in eng), Phys Med Biol, vol. 62, no. 12, pp. 5098-5113, Jun 2017, doi: 10.1088/1361-6560/aa6cf1.
[8] L. Sinha et al., "Quantification of the binding potential of cell-surface receptors in fresh excised specimens via dual-probe modeling of SERS nanoparticles," (in eng), Sci Rep, vol. 5, p. 8582, Feb 2015, doi: 10.1038/srep08582.
[9] K. S. Samkoe, K. M. Tichauer, J. R. Gunn, W. A. Wells, T. Hasan, and B. W. Pogue, "Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach," (in eng), Cancer Res, vol. 74, no. 24, pp. 7465-74, Dec 2014, doi: 10.1158/0008-5472.CAN-14-0141.
[10] K. M. Tichauer et al., "Accounting for pharmacokinetic differences in dual-tracer receptor density imaging," (in eng), Phys Med Biol, vol. 59, no. 10, pp. 2341-51, May 2014, doi: 10.1088/0031-9155/59/10/2341.
[11] K. M. Tichauer, K. S. Samkoe, K. J. Sexton, J. R. Gunn, T. Hasan, and B. W. Pogue, "Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging," (in eng), J Biomed Opt, vol. 17, no. 6, p. 066001, Jun 2012, doi: 10.1117/1.JBO.17.6.066001.
[12] K. M. Tichauer et al., "In vivo quantification of tumor receptor binding potential with dual-reporter molecular imaging," (in eng), Mol Imaging Biol, vol. 14, no. 5, pp. 584-92, Oct 2012, doi: 10.1007/s11307-011-0534-y.
[13] B. Meng et al., "Noninvasive quantification of target availability during therapy using paired-agent fluorescence tomography," (in eng), Theranostics, vol. 10, no. 24, pp. 11230-11243, 2020, doi: 10.7150/thno.45273.
[14] N. Sadeghipour et al., "Prediction of optimal contrast times post-imaging-agent-administration to inform personalized fluorescence-guided surgery," J Biomed Opt, vol. Accepted, 2020.
[15] N. Sadeghipour, S. C. Davis, and K. M. Tichauer, "Correcting for targeted and control agent signal differences in paired-agent molecular imaging of cancer cell-surface receptors," (in eng), J Biomed Opt, vol. 23, no. 6, pp. 1-11, Jun 2018, doi: 10.1117/1.JBO.23.6.066004.
[16] N. Sadeghipour, S. C. Davis, and K. M. Tichauer, "Quantifying cancer cell receptors with paired-agent fluorescent imaging: a novel method to account for tissue optical property effects," (in eng), Proc SPIE Int Soc Opt Eng, vol. 10497, 2018 Jan-Feb 2018, doi: 10.1117/12.2290631.
[17] N. Sadeghipour, S. C. Davis, and K. M. Tichauer, "Generalized paired-agent kinetic model for in vivo quantification of cancer cell-surface receptors under receptor saturation conditions," (in eng), Phys Med Biol, vol. 62, no. 2, pp. 394-414, 01 2017, doi: 10.1088/1361-6560/62/2/394.
[18] S. C. Davis and K. M. Tichauer, "Small-Animal Imaging Using Diffuse Fluorescence Tomography," (in eng), Methods Mol Biol, vol. 1444, pp. 123-37, 2016, doi: 10.1007/978-1-4939-3721-9_12.
[19] S. C. Davis et al., "Dynamic dual-tracer MRI-guided fluorescence tomography to quantify receptor density in vivo," (in eng), Proc Natl Acad Sci U S A, vol. 110, no. 22, pp. 9025-30, May 2013, doi: 10.1073/pnas.1213490110.
[20] K. M. Tichauer et al., "Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging," (in eng), Nat Med, vol. 20, no. 11, pp. 1348-53, Nov 2014, doi: 10.1038/nm.3732.