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このたび、Dr. Dasの論文が学術誌に掲載されました。
Dr. Das's paper has recently been published in an academic journal.

Journal：Plant Stress

Title：Sensitive detection of Plantago asiatica mosaic virus infection in Arabidopsis thaliana based on shifts in carotenoid resonance Raman bands

DOI：https://doi.org/10.1016/j.stress.2026.101314

Received 25 April 2025; Received in revised form 21 February 2026; Accepted 23 February 2026

Abstract

To improve the efficiency of plant virus disease management, novel strategy for early and sensitive detection of viral infection is essential. Raman spectroscopy (RS) which can provide specific signatures based on molecular vibrations from virus-infected plant tissue has recently attracted attention. In this study, we demonstrate that resonance Raman spectroscopy of carotenoids can be utilized to monitor the progression of Plantago asiatica mosaic virus (PlAMV) infection in Arabidopsis thaliana with enhanced sensitivity. We observed unidirectional shifts of approximately 1 cm−1 in ν1, ν2 and ν3 bands of carotenoids in the PlAMV infected plants compared with healthy plants. These spectral shifts in carotenoid bands are essentially associated to PlAMV infection as shown by a serological detection method ELISA. Intriguingly, such shifts in carotenoid bands emerged even before the infection was detected by ELISA, which emphasize the ability of resonance Raman spectroscopy for early detection of PlAMV infection. These subtle shifts in carotenoids bands for PlAMV infected plants can be correlated to alteration in carotenoid-lipid interactions, photosynthetic activities and redox status as revealed by transcriptomic analysis. Overall, the spectrally-shifted components of carotenoid bands allowed us to depict the progression of PlAMV infection in Arabidopsis thaliana with enhanced sensitivity.