Lab Members

What I'm doing:

I have the opportunity to work with cutting-edge technologies and techniques used in the study of the brain and nervous system. I assisted in various experiments involving animal models, tissue samples, and cell cultures. 

Some of the key experiences include:

Animal Handling and Behavioral Experiments: I'm learning how to handle and care for experimental animals, ensuring their well-being and adhering to ethical guidelines. I actively participate in behavioral experiments to study the effects of drugs, genetic modifications, or environmental factors on animal behavior. This involves designing and implementing experiments, conducting tests such as the Morris water maze, open field, and fear conditioning, and recording and analyzing the data using AnyMaze Video tracking software. 

Animal Cell Culture: I also culture and maintain different types of cells, including neuronal cells. I learned sterile techniques for cell culture, optimized cell growth conditions, cell counting, cell viability testing, and studying protein expression patterns.

Molecular Lab Experience: In the molecular lab, I focused on studying the intricate molecular processes underlying neural function. I worked with DNA, RNA, proteins, and various molecular biology techniques. Some of the highlights of my molecular lab experience include:

• DNA and RNA Analysis: I performed DNA and RNA extractions from various samples, including cells and tissues. I gained proficiency in techniques like polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR), gel electrophoresis, and DNA sequencing. These techniques were crucial for studying gene expression, genetic mutations, and regulatory mechanisms.

• Protein Analysis: I acquired skills in protein analysis techniques such as western blotting, immunofluorescence, and immunohistochemistry. These methods allowed me to investigate protein expression levels, localization, and interactions, aiding in the study of molecular pathways and protein function in the context of neurobiology.

My experiences in the neuro and molecular lab have been invaluable in deepening my understanding of neurobiology and molecular biology. I have developed a strong foundation in laboratory techniques, data analysis, and scientific reasoning. This hands-on experience has further fueled my passion for research and solidified my commitment to pursuing a career in neuroscience or molecular biology. I am excited to continue exploring the fascinating world of neurobiology and contribute to the advancement of knowledge in this field.

What I'm doing:

Exploring the Therapeutic Role of Sulforaphane in Alzheimer’s Disease

I am Vajinder Kaur, a dedicated PhD researcher focused on understanding the potential of sulforaphane in treating Alzheimer’s disease (AD). My work aims to unravel the complex molecular mechanisms behind this neurodegenerative disorder, particularly by examining miRNA differential expression in an Alzheimer’s disease mouse model.

Through my research, I strive to contribute to innovative therapeutic approaches that could pave the way for new treatment strategies.

Research Techniques & Methodologies

To achieve my research goals, I employ a range of advanced techniques, including:

RNA Sequencing (RNA-seq) – To analyze miRNA differential expression patterns.

Animal Model Studies – Using early-stage AD mouse models to study Alzheimer’s disease.

Molecular Biology Techniques – Including qRT-PCR, Western blotting, and ELISA for biomarker validation.

Bioinformatics Analysis – To decode gene expression data and pathway interactions.

Histopathology & Immunohistochemistry – To assess brain tissue alterations and protein expression.