李俊霖 博士
Dr. Chun-Lin Lee
Position:
- Professor of Department of Life Science, National Taitung University
- Center Chairman of East Taiwan Bio-Economic Center, National Taitung University
- Council board of Taiwan Society of Deep Ocean Water Resource Application.
Education and Degrees :
Ph.D., Institute of Microbiology and Biochemistry, National Taiwan University
Fields of Specialization :
Functional Microorganism Research
Research on Health Foods and Animal Testing for Health Food Evaluation
Development of Biotechnological Products Using Deep Ocean Water
Research and Development of Functional and Health Foods in Food Biotechnology
Pilot Production and Commercialization
Operation of Food Biotechnology Factories
Quality Management and Guidance for Custard Apple Agricultural Products
From Fermentation to Functional Microbiome
The Microbiology and Biochemistry Laboratory led by Professor Chun-Lin Lee at National Taitung University focuses on harnessing functional microorganisms and local plant resources to develop high-value functional foods. Our research integrates microbial fermentation with Taiwan’s unique agricultural products, such as roselle and red quinoa, to enhance bioactivity and functional ingredient content. Additionally, we employ deep ocean water to stimulate microbial activity, thereby increasing fermentation efficiency and product efficacy.
Our laboratory’s multi-faceted approach spans from foundational fermentation studies to advanced applications in multi-omics, including metabolomics, proteomics, and transcriptomics, to elucidate metabolic pathways and optimize fermentation processes. We also employ cutting-edge technologies such as 16S DNA next-generation sequencing to analyze gut microbiome dynamics in experimental models and explore the symbiosis of wild Antrodia cinnamomea. Moreover, our recent investigations into exosome research focus on analyzing microbial and plant-derived exosomes to assess their potential roles in immune modulation and anti-inflammatory functions.
A critical component of our research involves scaling up production through pilot-scale processing, which includes fermentation, extraction, freeze-drying, and microencapsulation. This process design and optimization ensure quality and stability for potential market applications. Through rigorous in vitro cell and in vivo animal model studies, we validate the health-promoting effects of our fermented products, laying the groundwork for innovative and scientifically validated functional foods. Our laboratory strives to combine cutting-edge research with sustainable use of local resources, aiming to bridge scientific innovation and practical applications in the functional food industry.
Academic and Work Experience:
Council board of Taiwan society of deep ocean water resource application (2014/09- current)
Chairman of Department of Life Science, National Taitung University (2013/01-2020/1)
Director of Science and Research center of Deep ocean water, National Taitung University (2014/08-2017/7)
Deputy director of Sun-Way Biotechnology company. (2017/11~2018/7)
Academic and Work Experience:
Council board of Taiwan society of deep ocean water resource application (2014/09- current)
Chairman of Department of Life Science, National Taitung University (2013/01-2020/1)
Director of Science and Research center of Deep ocean water, National Taitung University (2014/08-2017/7)
Deputy director of Sun-Way Biotechnology company. (2017/11~2018/7)
Awards
2010~2023 The Ministry of Science and Technology rewards special talents
2023 Nutrition and Health Food Innovation Award, Taiwan Health Food Society, (for Red Quinoa and Antrodia cinnamomea Mycelium Capsules)
2020 Outstanding Young Alumni Award, National Pingtung University of Science and Technology
2015 Food Science and Technology Society Academic Honor Award
Publication list
Yang, PX, Hsu, YW, Pan, TM*, Lee CL*. (2024). Comparative effects of monascin and monascinol on pro-inflammatory factors and histopathological alterations in liver and kidney tissues in a streptozotocin-nicotinamide induced rat model. Journal of Fungi. (accept) (SCI)
Liu CF, Young ZY, Shih TW, Pan TM*, Lee CL* . 2024 Lactocaseibacillus-deglycosylated isoflavones prevent Aβ40-induced Alzheimer's disease in a rat model. AMB Express, 14(1), 90. https://doi.org/10.1186/s13568-024-01735-y (SCI)
Liu CF, Chao WY, Shih TW, Lee CL*, Pan TM*. 2024. Enhancement of Regulatory T Cell Maturation and Th1/Th2 Balance through FOXP3 Expression by Lactobacillus paracasei in an Ovalbumin-Induced Allergic Skin Animal Model. Curr. Issues Mol. Biol. (accept) (SCI)
Kim S, Na GH, Yim DJ, Liu CF, Lin TH, Shih TW, Pan TM, Lee CL*, Koo YK*. 2024. Lactobacillus paracasei subsp. paracasei NTU 101 prevents obesity by regulating AMPK pathways and gut microbiota in obese rat. Biochem Biophys Res Commun. 2024, 30;731:150279. doi: 10.1016/j.bbrc.2024.150279. (SCI)
Yang PX, Hsu YW, Pan TM*, Lee CL*. 2024. Monascinol from Monascus pilosus-Fermented Rice Exhibits Hypolipidemic Effects by Regulating Cholesterol and Lipid Metabolism. J Func Food, 104, 105537. https://doi.org/10.1016/j.jff.2023.105537 (SCI)
Liu CF, Pan CM, Lee CL*. 2024. The Mass-Produced Compound Extract from Antlered form of Ganoderma Lucidum and Red Quinoa Effectively Protects the Liver by Antioxidant and Anti-Inflammatory Mechanisms in Carbon Tetrachloride Induced Liver Fibrosis Animal Model. J Biotech Biores. 5(3). JBB. 000613. DOI: 10.31031/JBB.2024.05.000613
Liu CF, Zhang XF, Yu TL, Lee CL*. 2024. Utilizing Deep Ocean Water in Yeast Fermentation for Enhanced Mineral-Rich Biomass Production and Fermentative Regulation by Proteomics Modulation. Heliyon. 10(10) 30, e31031 https://doi.org/10.1016/j.heliyon.2024.e31031 (SCI)
Liu CF, Shih TW, Lee CL* Pan TM*. 2024. The Beneficial Role of Lactobacillus paracasei subsp. paracasei NTU 101 in the Prevention of Atopic Dermatitis. Curr. Issues Mol. Biol., 46(3), 2236-2250; https://doi.org/10.3390/cimb46030143 (SCI)
Xu TY, Tzeng DY, Lee CL*. 2023. Using Deep Ocean Water in the Fermentation of Antrodia cinnamomea to Boost Magnesium Ion Bioabsorption and Anti-Inflammatory Effects in the Brain of an Alzheimer’s Disease Rat Model. Fermentation, 9(10), 893; https://doi.org/10.3390/fermentation9100893 (SCI)
Chang CY, Yang PX, Yu TL, Lee CL*. 2023. Cordyceps cicadae NTTU 868 Mycelia fermented with deep ocean water minerals prevents d-galactose-induced memory deficits by inhibiting oxidative inflammatory factors and aging-related risk factors. Nutrients, 15(8), 1968; https://doi.org/10.3390/nu15081968 (SCI)
Lin CW, Lin CH, Y, Hsu YW, Pan TM*, Lee CL*. 2023. Monascin and ankaflavin prevents metabolic disorder by blood glucose regulatory, hypolipidemic, and anti-inflammatory effects in high fructose and high fat diet-induced hyperglycemic rat. Journal of Functional Foods, 104, 105537 (2023.04) (SCI)
Lin CW, Lin PY, Y, Hsu YW, Pan TM*, Lee CL*. 2023. Monascus-fermented metabolites repressed amyloid β-peptide-induced neurotoxicity and inflammatory response in in vitro and in vivo studies. Journal of Functional Foods, 104, 105509. (2023.03) DOI: 10.1016/j.jff.2023.105509 (SCI,)
Lin CH, Huang HL, Chen YH, Lee CL*. 2022. Deep Ocean Water Minerals Promotes the Growth and Cordycepin Production of Cordyceps militaris Fruiting Bodies through Proteomics Regulation. Fermentation. 2022, 8, 481. https://doi.org/10.3390/fermentation8100481 (SCI)
Lin TA, Ke BJ, Cheng CS, Lee CL*. 2021. Red Quinoa Bran Extract Prevented Alcoholic Fatty Liver Disease via Increasing Antioxidative System and Repressing Fatty Acid Synthesis Factors in mice fed Alcohol Liquid Diet. Molecules. 18;26(22):6973. doi: 10.3390/molecules26226973. (SCI, IF:4.412)
Lai JR, Hsu YW, Pan TM*, Lee CL*. 2021. Monascin and ankaflavin of Monascus purpureus prevent alcoholic liver disease through regulating AMPK-mediated lipid metabolism and enhancing anti-inflammatory and anti-oxidative systems. Molecules. 26(20), 6301; https://doi.org/10.3390/molecules26206301 (SCI, IF:4.412)
Lee CY, Lee CL*. 2021. Comparison of the improvement effect of deep ocean water with different mineral composition on the high fat diet-induced blood lipid and nonalcoholic fatty liver disease in a mouse model. Nutrients, 13(5), 1732; doi.org/10.3390/nu13051732 (SCI)
Wu YZ, Lee CL*. 2021. Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the Aβ40 and Streptozotocin-induced Memory Deficit via Suppressing Alzheimer’s Disease Risk Factors and Increasing Magnesium Uptake of Brain. Fermentation 7(1), 39 (SCI).
Ke BJ, Lee CL*. 2019. Using submerged fermentation to fast increase N6-(2-hydroxyethyl)-adenosine, adenosine and polysaccharide productions of Cordyceps cicadae NTTU 868. AMB express 9:198 (SCI).
Lai JR, Ke BJ, Hsu YW, Lee CL*. 2019. Dimerumic acid and deferricoprogen produced by Monascus purpureus attenuate liquid ethanol diet -induced alcoholic hepatitis via suppressing NF-κB inflammation signalling pathways and stimulation of AMPK-mediated lipid metabolism. J Func Food. 60, 103393 (SCI).
Lin TA, Ke BJ, Cheng CS, Wang JJ, Wei BL, Lee CL*. 2019. Red quinoa bran extracts protects against carbon tetrachloride-induced liver injury and fibrosis in mice via activation of antioxidative enzyme systems and blocking TGF-β1 pathway. Nutrients. 11:395 (SCI).
Ke BJ, Lee CL*. 2018. Cordyceps cicadae NTTU 868 mycelium prevents CCl4-induced hepatic fibrosis in BALB/c mice via inhibiting the expression of pro-inflammatory and pro-fibrotic cytokines. J Func Food 43: 214-223 (SCI).
Lee CL, Pan TM*. 2017. The prevention of Alzheimer's disease and Parkinson’s disease by Monascus purpureus NTU 568-fermented compounds. J Alzheimers Dis Parkinsonism. 7:4 DOI: 10.4172/2161-0460.1000342
Hung YP, Lee CL*. 2017. Higher Anti-Liver Fibrosis Effect of Cordyceps militaris-Fermented Product Cultured with Deep Ocean Water via Inhibiting Proinflammatory Factors and Fibrosis-Related Factors Expressions. Mar Drugs. 8:15. pii: E168. doi: 10.3390/md15060168.
Wang JJ, Wu CC, Lee CL, Hsieh SL, Chen JB, Lee CI*. 2017. Antimelanogenic, Antioxidant and Antiproliferative Effects of Antrodia camphorata Fruiting Bodies on B16-F0 Melanoma Cells. PLoS One. 12(1) :e0170924. doi: 10.1371/journal.pone.0170924.
Lee CL, Wen JY, Hsu YW, Pan TM. The blood lipid regulation of Monascus-produced monascin and ankaflavin via the suppression of low-density lipoprotein cholesterol assembly and stimulation of apolipoprotein A1 expression in the liver. J Microbiol Immunol Infect. 2018 Feb;51(1):27-37. doi: 10.1016/j.jmii.2016.06.003. Epub 2016 Jun 24.
Lung TY, Liao LY, Wang JJ, Wei BL, Huang PY, Lee CL*. 2016. Metals of deep ocean water increase the anti-adipogenesis effect of Monascus-fermented product via modulating the monascin and ankaflavin production. Mar Drugs. 14(6). pii: E106. doi: 10.3390/md14060106. (online) (SCI)
Shia YC, Yang SY, Lee DY, Lee CL*. 2016. Increasing Anti-Aβ-induced neurotoxicity ability of Antrodia camphorata-fermented product with deep ocean water supplementary. J Sci Food Agri. 96: 4690–4701 DOI: 10.1002/jsfa.7687 (online) (SCI)
Lee CL*. 2015. The advantages of deep ocean water for the development of functional fermentation food. Appl Microbiol Biotechnol. 99: 2523-2531. DOI: 10.1007/s00253-015-6430-7 (SCI)
Lee CL, Lin PY, Hsu YW, Pan TM. 2015. Monascus-fermented monascin and ankaflavin improve the memory and learning ability in amyloid β-protein intracerebroventricular-infused rat via the suppression of Alzheimer’s disease risk factors. J Func Food 18:387-399 (SCI)
Hung YP, Wang JJ, Wei BL, Lee CL*. 2015. Effect of the salts of deep ocean water on the production of cordycepin and adenosine of Cordyceps militaris-fermented product. AMB express. 5:53. DOI 10.1186/s13568-015-0140-5 (SCI)
Lee CI, Wu CC, Hsieh SL, Lee CL, Chang YP, Chang CC, Wang YZ, Wang JJ. 2014. Anticancer effects on human pancreatic cancer cells of triterpenoids, polysaccharides and 1,3-β-d-glucan derived from the fruiting body of Antrodia camphorata. Food Funct. 5: 3224-3232. (SCI)
Wang LC, Lung TY, Kung, YH, Wang JJ, Tsai TY, Wei BL, Pan TM, Lee CL*. 2013. Enhanced anti-obesity activities of red mold dioscorea when fermented using deep ocean water as the culture water. Mar Drugs. 11: 3902-3925.
Wang LC, Kuo IU, Tsai TY, Lee CL*. 2013. Antrodia camphorata-fermented product cultured in deep ocean water has more liver protection against thioacetamide-induced fibrosis. Appl Microbiol Biotechnol. 97: 9955-9967. (SCI)
Lee CL, Wen JY, Hsu YW, Pan TM. 2013. Monascus-fermented yellow pigments monascin and ankaflavin showed antiobesity effect via the suppression of differentiation and lipogenesis in obese rats fed a high-fat diet. J Agric Food Chem. 61: 1493-1500.
Lee CL, Hung YP, Hsu YW, Pan TM. 2013. Monascin and ankaflavin have more anti-atherosclerosis effect and less side effect involving increasing creatinine phosphokinase activity than monacolin K under the same dosages. J Agric Food Chem. 61: 143-150.
Lee CI, Lee CL, Hwang JF, Lee YH, Wang JJ. 2013. Monascus-fermented red mold rice exhibits cytotoxic effect and induces apoptosis on human breast cancer cells. Appl Microbiol Biotechnol. 97: 1269-1278 (SCI)
Wang LC, Wang SE, Wang JJ, Tsai TY, Lin CH, Pan TM, Lee CL*: 2012. In vitro and in vivo comparisons of the effects of the fruiting body and mycelium of Antrodia camphorata against amyloid β-protein-induced neurotoxicity and memory impairment. Appl Microbiol Biotechnol. 94: 1505-1519 (SCI) (NSC-97-2321-B-143-001-MY2)
Lee CL, Pan TM. 2012. Development of Monascus fermentation technology for high hypolipidemic effect. Appl Microbiol Biotechnol. 94: 1449-1459 (SCI)
Lee CL, Kung, YH, Wang JJ, Lung TY, Pan TM: 2011, Enhanced hypolipidemic effect and safety of red mold dioscorea cultured in deep ocean water. J Agric Food Chem. 59: 8199-8207. (SCI) (NSC-98-2313-B-143-002-MY3)
Lee CL, Pan TM: 2011. Red mold fermented products and Alzheimer´s disease. Appl Microbiol Biotechnol. 91: 461-469. (SCI) (NSC-99-2622-B-143-001-CC3)
Wu CL, Kuo YH, Lee CL, Hsu YW, Pan TM: 2011, Synchronous high-performance liquid chromatography with a photodiode array detector and mass spectrometry for the determination of citrinin, monascin, ankaflavin, and the lactone and acid forms of monacolin K in red mold rice. J AOAC Int. 94: 179-190. (SCI)
Lee CL, Kung YH, Wu CL, Hsu YW, Pan TM: 2010, Monascin and ankaflavin act as novel hypolipidemic and high-density lipoprotein cholesterol-raising agents in red mold dioscorea. J Agric Food Chem. 58: 9013–9019. (SCI) (NSC-98-2313-B-143-002-MY3)
Lee CL, Kuo TF, Wu CL, Wang JJ, Pan TM: 2010, Red mold rice promotes neuroprotective sAPPalpha secretion instead of Alzheimer’s risk factors and amyloid beta expression in hyperlipidemic Aβ40-infused rats. J Agric Food Chem. 58: 2230–2238. (SCI)
Lee HC, Lee CL, Pan TM. 2010. A 90-Day toxicity study of Monascus–fermented products including high citrinin level. J Food Sci.75: T91-T97.(SCI)
Wu CL, Lee CL, Pan TM: 2009, Red mold dioscorea has greater anti-hypertensive effect than traditional red mold rice in spontaneously hypertensive rats. J Agric food Chem. 57: 5035-5041. (SCI)
Tsai TY, Chu LH, Lee CL, Pan TM: 2009, Atherosclerosis-preventing activity of lactic acid bacteria-fermented milk-soymilk supplemented with Momordica charantia. J Agric Food Chem. 57: 2065–2071. (SCI)
Lee CL, Wang JJ, Pan TM: 2008, Red mold rice extract represses amyloid beta peptide-induced neurotoxicity via potent synergism of anti-inflammatory and anti-oxidative effect. Appl Microbiol Biotechnol. 79: 829-841. (SCI)
Chen WP, Ho BY, Lee CL, Lee CH, Pan TM: 2008, Red mold rice prevents the development of obesity, dyslipidemia and hyperinsulinemia induced by high-fat diet. Int J Obesity. 32: 1694-1704. (SCI)
33. Yu CC, Wang JJ, Lee CL, Lee SH, Pan TM: 2008, Safety and mutagenicity evaluation of nanoparticulate red mold rice. J Agric Food Chem. 56: 11038-11048.
Lee CL, Kuo TF, Wang JJ, Pan TM: 2007, Red mold rice ameliorates impairment of memory and learning ability in intracerebroventricular amyloid beta-infused rat via repressing amyloid beta accumulation. J Neurosci Res. 85: 3171-3182. (SCI) (NSC95-2313-B-002-019)
Lee CL, Hung HK, Wang JJ, Pan TM: 2007, Red mold dioscorea has greater hypolipidemic and anti-atherosclerotic effect than traditional red mold rice and unfermented dioscorea in hamster. J Agric Food Chem. 55: 7162-7169. (SCI)
Lee CL, Chen WP, Wang JJ, Pan TM: 2007, A simple and rapid approach for removing citrinin while retaining monacolin K in red mold rice. J Agric food Chem. 55: 11101–11108. (SCI).
Lee CL, Hung HK, Wang JJ, Pan TM: 2007, Improving the ratio of monacolin K to citrinin production of Monascus purpureus NTU 568 under dioscorea medium through the mediation of pH value and ethanol addition. J Agric Food Chem. 55: 6493-6502. (SCI)
Lee CL, Wang JJ, Kuo SL, Pan TM: 2006; Monascus fermentation of dioscorea for increasing the production of cholesterol-lowering agent-monacolin K and antiinflammation agent-monascin. Appl Microbiol Biotechnol. 72: 1254-1262. (SCI)
Lee CL, Tsai TY, Wang JJ, Pan TM: 2006, In vivo hypolipidemic effects and safety of low dosage Monascus powder in a hamster model of hyperlipidemia. Appl Microbiol Biotechnol. 70: 533-540. (SCI)
Lee CL, Wang JJ, Pan TM: 2006; Synchronous analysis method for detection of citrinin and the lactone and acid forms of monacolin K in red mold rice. J AOAC Int. 89: 669-677. (SCI)
Yu CC, Lee CL, Pan TM: 2006; A novel formulation approach for preparation of nanoparticulate red mold rice. J Agric Food Chem. 54: 6845-6851. (SCI)
Chang CY, Lee CL, Pan TM: 2006; Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures. Appl Microbiol Biotechnol. 72: 654-661. (SCI)
Wang JJ, Shieh MJ, Kuo SL, Lee CL, Pan TM: 2006, Effect of red mold rice on antifatigue and exercise-related changes in lipid peroxidation in endurance exercise. Appl Microbiol Biotechnol. 70: 247-253. (SCI)
Wang JJ, Lee CL, Pan TM: 2004 Modified mutation method for screening low citrinin-producing strains of Monascus purpureus on rice culture. J Agric Food Chem.; 52: 6977-6982. (SCI)
Wang JJ, Lee CL, Pan TM: Improvement of monacolin K, gamma-aminobutyric acid and citrinin production ratio as a function of environmental conditions of Monascus purpureus NTU 601. J Ind Microbiol Biotechnol. 2003; 30: 669-676. (SCI)
Patent
US Patent
Method of treatment of preventing hyperglycemia complications (patent)
Pan, Tzu-Ming | Lee, Chun-Lin | Hsu, Ya-Wen
Patent Number: 10143678 (2018)
Cite | Source
Method for manufacturing composition for lowering blood lipid and elevating high-density lipoprotein (patent)
Pan, Tzu-Ming | Lee, Chun-Lin | Wu, Cheng-Lun
Patent Number: 9364457 (2016)
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Method for manufacturing composition for lowering blood lipid and elevating high-density lipoprotein (patent)
Pan, Tzu-Ming | Lee, Chun-Lin | Wu, Cheng-Lun
Patent Number: 9358221 (2016)
Cite | Source
Method for manufacturing red mold dioscorea (patent)
Patent Number: 8703456 (2014)
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Method for manufacturing red mold dioscorea (patent)
Patent Number: 8697424 (2014)
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Method for prevention and treatment of Alzheimer's disease (patent)
Pan, Tzu-Ming | Lee, Chun-Lin
Patent Number: 8097259 (2012)
Cite | Source
Korea patent: Composition and method for prevention and treatment of Alzheimer´s disease. Korea, 10-0959001, 2010/05/12
Singapore patent: Composition and method for prevention and treatment of Alzheimer´s disease. Singapore, 200719013-5, 2010/10/29.
USA patent: Composition and method for prevention and treatment of Alzheimer´s disease. USA, 11963533, 2012.01
Canada patent: Composition and method for prevention and treatment of Alzheimer´s disease. Canada, 2616971, 2012.07
Japan patent: Pan TM, Lee CL. Composition and method for prevention and treatment of Alzheimer´s disease. Japan, 特許第4974031, 2012.04
Korea patent: Method for manufacturing red mold dioscorea. Korea, 10-1227332, 2013.01.
Japan patent: Method for manufacturing red mold dioscorea.日本，特許第4948609，2012.3
Taiwan patent: Composition and method for prevention and treatment of Alzheimer´s disease. Taiwan, ROC, I341204, 2011.05.01
Taiwan patent: Method for manufacturing red mold dioscorea. Taiwan, ROC, I-350733. 2011/10/21.
European patent: Method for manufacturing a composition for lowering blood lipid and elevating high-density lipoprotein. European Union。EP 2559433 B1
European patent: Composition for Lowering Blood Lipid and Elevating High Density Lipoprotein and Method for Manufacturing the Same. European Union. EP 2559433 2013/11
Korea patent: Composition for Lowering Blood Lipid and Elevating High Density Lipoprotein and Method for Manufacturing the Same. Korea, 10-1420904, 2014/7
Canada patent: Composition for Lowering Blood Lipid and Elevating High Density Lipoprotein and Method for Manufacturing the Same. Canada. CA 2826966.
Singapore patent Composition for Lowering Blood Lipid and Elevating High Density Lipoprotein and Method for Manufacturing the Same. Singapore. SG187997. 2014/11/19
Taiwan patent: Method for promoting the production of catechins, catechols and chlorogenic acid by using deep seawater to promote lactobacillus plantarum to produce catechins, catechols and chlorogenic acid. 2016/1 (I518181)
Taiwan patent: Use of deep seawater lactic acid bacteria fermentation product in preparing composition for reducing body fat formation. 2019/7 (I664971)