Mitochondrial Gene Variation between Mainland and Coastal Amphiuma might indicate reduced diversity in barrier islands

REU fellow: Denisse Partida, Georgia Southern University

Mentors: Dr. Scott Harrison, Dr. Checo Colón-Gaud

Amphiuma are large freshwater salamanders that can reach lengths of 460-1160 mm. Individuals of the species A. means are characterized by four small limbs with two toes on each extremity. These large salamanders are typically found in areas of shallow, heavily vegetated water and their distribution range expands throughout southeastern North America. We collected tail clips from individuals of Amphiuma at various sites within two locations in southeast Georgia (Fort Stewart on the mainland and Sapelo Island on the coast) to assess the genetic diversity of inland vs. coastal populations. Sapelo Island is a barrier island separated from the mainland by saltwater. Sapelo Island is at risk of saltwater intrusion from heavy winds and rising sea levels possibly affecting the populations of Amphiuma means as they have limited tolerance to salinity. We selected the genes 16s and cytochrome b to assess for mitochondrial variation between individuals sampled at inland Ft. Stewart vs. coastal Sapelo Island. We found differences in haplotypes (alleles) present in individuals from inland vs. coastal sites suggesting genetic variation between these sub-populations.