eDNA Extraction & Quantification
The tax document analysis returned data on the enslaved population with categories for able men, able women, other enslaved populations, and the total enslaved population for each estate. The average total enslaved population from 1772 to 1817 was approximately 130, with values ranging from 24 to 368. Betty’s Hope and Bethlehem Old Works had the largest average enslaved population, with Bethlehem Old Works having the largest number of enslaved people (368) in 1780, while Adventure and Great Pond had the smallest average. The total average enslaved population across all analyzed estates peaked in 1782 and was at its lowest in 1817, the last year of our data set.
Average DNA Concentration between all Archived & Fresh (Control) Soil Samples
Note. Archived soil samples are in blue while fresh (control) soil samples are in orange. Archived average was taken from archived soil samples #1-5 (OG1), #21-23 (OG2), & #36-38 (SG2). Fresh average was taken from 3 soil samples collected at UCLA (C1, C2, C3).
Note. The average DNA concentration in archived soil samples from OG2 (x̄ = 13.2 ng/μL) is greater than the samples from OG1 (x̄ = 5.16 ng/μL) and SG2 (x̄ = 4.5 ng/μL).
Note. The average DNA concentration in OG (x̄ = 8.17 ng/μL) vs. in SG (x̄ = 4.5 ng/μL). p = 0.14 > 0.05 shows that the difference in average DNA concentration between OG and SG is not significant.
DNA Concentration in ELP Archived Soil Samples
Note. Highlighted in blue are the archived soil samples from ELP and highlighted in orange are the fresh (control) soil samples from UCLA.
Figure 7
Note. DNA concentration of C1 is a negative number.
Figure 8
Figure 9
DNA Concentration in Archived Soil Samples from SG2 Plot and Fresh (Control) Soil Samples from Franz Hall at UCLA
Figure 10
DNA Concentration between OG (#1-5, 21-23) and SG Samples (#36-38) by Depth
Note. Units of depth were not provided in the metadata. Only OG samples were averaged for DNA concentration since there was more than 1 sample at each depth.
PCR & Gel Electrophoresis
Figure 11
Note. The DNA from the samples after PCR is shown to be 300bp.
Figure 12a
Note. All samples except #1, 3, 5, 24, & B3 amplified. The DNA from the samples after PCR indexing is shown to be 400-500bp.
Figure 12b
Note. The DNA from samples #1, 3, 5, 24, & B3 after PCR indexing is shown to be 350-400bp.
Figure 13
DNA Concentration in PCR Indexed Archived Soil Samples from ELP and Fresh (Control) Soil Samples at UCLA
Note. We introduced blanks (B1, B2, and B3), which are shaded in gray. This means they have PCR indexes but not soil eDNA.
Figure 14
Calculating quantities of cleaned, indexed PCR to pool
Final volume desired = 300 uL
Desired concentration of pool = 62.96 nM
Number of samples = 17
Length of indexing adapter = 135 base pairs
Figure 15
Figure 16
Spatial Analysis