Research

Unraveling the computational principles of the brain using optical technology

I want to elucidate how the brain sees, thinks, and acts using optical technologies such as two-photon calcium imaging and two-photon optogenetics.

Large-field two-photon microscope

In my doctoral program, I am conducting research aimed at elucidating the computational principles of the brain, using mice as subjects, through the development of microscopy techniques and the measurement of neural activity during behavioral experiments.

We have been working on the construction and expansion of Diesel2p (Yu et al., 2021), a fully open-source large-field two-photon microscope developed by the Spencer Smith Laboratory in the United States.

We developed FPGA-based open-source software and a new laser multiplexing method, successfully recording the activity of over 10,000 neurons across the entire cerebral cortex simultaneously at 4 different depths. Additionally, we demonstrated large-field fluorescence lifetime imaging using the same system.

bioRxiv. https://doi.org/10.1101/2025.08.13.669807

3D cell tracking and light stimulation

In my undergraduate and master's programs, I worked on developing a system for tracking and optically stimulating neurons with single-cell resolution in free-moving C.elegans.

I learned optics and computer science technology such as femtosecond pulse laser construction, two-photon microscope design and construction, optical wavefront shaping using spatial light modulators (SLMs), and high-speed image processing.