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Is 1st layer gel flat?
Is 1st layer gel flat?
- Fluorescent bead solution was settled on top of 1st gel layer created.
- If flat, all fluorescence should be detected on the same focal plane under microscope.
- Our 1st gel layer turned to be flat after treatment with oil layer.
Top: Fluorescent beads were added to PA solution II.
Bottom: Beads settled on top of completed gel.
Correct volume of PA solution II for 1st gel rehydration?
Correct volume of PA solution II for 1st gel rehydration?
- If PA solution II added is not enough, 1st layer gel would not be rehydrated.
- If PA solution II added is too much, it will create an extra layer on top of 1st layer gel after UV cure, which interferes with the soft/stiff patterning.
- So, need to make sure volume of PA solution II added is just right.
- Fluorescent beads were added to PA solution II, then added on top of 1st gel layer.
- Beads are too large to travel down 1st layer gel matrix.
- If PA solution II added is too much, fluorescent beads would be distributed in extra gel layer on top of 1st layer, and would not be detected on the same focal plane under microscope.
- If PA solution II added just rehydrated 1st gel layer, all fluorescent beads would be settle on top of gel created, and would be detected on the same focal plane under microscope.
- Our beads were on same focal plane under microscope, meaning me have added the correct volume of PA solution II.
Testing Patterning Results
Testing Patterning Results
- Soft stripes (dark): avg width = 193.7 um
- Stiff stripes (light): avg width = 581.2 um
- Soft and stiff stripes should both be ~390um as designed
- Reasoning: diffraction increases as gel height increases, resulting in larger width of stiff stripes created.
- Atomic Force Microscopy: YM= 614.3Pa
Can cells survive on hydrogel we made?
Can cells survive on hydrogel we made?
- 3T3 cells are more robust and grows faster.
- Cardiomyocytes are extremely sentitive and grows slower.
- In this stage of our project, 3T3 cells were used instead of cardiomyocytes.
- After culturing on the hydrogel we created, 3T3 cells showed healthy shapes.
Page Leader: Jingwen Lu
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