Results to date
Results to date
Embedded Files
We have made ground breaking discoveries even with ongoing insurmountable constraints.
Result1: Confirm Activation of Ferritin Reporter after Co-culture
Increased fluorescence intensity (20X) after co-culturing with CD19+ Toledo tumor cells
Figure 1: Fluorescence intensity measurement from flowcytometry (left) and con-focal microscope (right). Co-culture was performed with 2 different tumor cells: antigen CD19- K562 (as negative control) and antigen CD19+ Toledo.
Result2: Confirm Ferritin’s Function of Iron Storage Inside Cells
Cells can successfully store iron ions inside after co-culturing with CD19+ Toledo tumor cells
Figure 2: Prussian Blue staining results for different co-culture groups. Left to right: JurkatWT only, engineered Jurkatonly, engineered Jurkatwith CD19- K562 tumor cells, engineered Jurkatwith CD19+ Toledo tumor cells. Top to bottom: pure medium, medium with 200nm ferric solution, medium with 500nm ferric solution. FAC=ferric ammonium citrate
Motivation: Why go to 5 or 6 stores when you can go to 1?
Motivation: Why go to 5 or 6 stores when you can go to 1?
Can we use just 1 imaging modality?
Can we use just 1 imaging modality?
MRI in vivo using naturally produced contrast agent will supplant the necessity of 5 or 6 other procedures.
We have come so far, yet not far enough. The next generation of this project must move on to MRI studies and examine the effects of our innovative methodology. Will you be the 1 to take up this task?
We have come so far, yet not far enough. The next generation of this project must move on to MRI studies and examine the effects of our innovative methodology. Will you be the 1 to take up this task?
Design 1 (DS1) ferritin reporter with upstream activation sequence and mNeonGreenreporter
Page Leader: Mitchell Kong
Page updated
Report abuse