Embedded Files
Overview
Overview
Our design is divided into 2 subprojects:
Our design is divided into 2 subprojects:
1) Creating a micropatterned polydimethylsiloxane (PDMS) membrane that allows for aligned stem cell cardiomyocyte culturing.
2) Finding an optimal x-ray compatible material as a substrate for modified petri dishes.
Ultimately, the goal was to create a prototype that combines the two subproject design - a petri dish with an adhered patterned hydrogel membrane with cultured cardiomyocytes covered by a Teflon or Kapton coverslip.
Subproject 1: Creation and adherence of a micropatterned membrane to modified petri dish
Subproject 1: Creation and adherence of a micropatterned membrane to modified petri dish
Key steps
Key steps
Prototype
Prototype
Our prototype for Subproject 1 is a modified petri dish with the bottom glass replaced with our micropatterned PDMS membrane. This way, hiPSCs can be cultured in a normal petri dish setting following a standard cell culture protocol.
Subproject 2: Finding an optimal x-ray compatible material
Subproject 2: Finding an optimal x-ray compatible material
Key steps
Prototype
Prototype
For Subproject 2, we finally removed the glass center bottom of normal petri dishes, attached our candidate materials -Kepton and Teflon of various thicknesses - and created a batch of prototypes as is shown in the picture. They are sent to Argonne National Laboratory in Chicago for XRD imaging to help us determine which material is the most x-ray compatible.
Page edited by: Rongjie Wu
Page updated
Report abuse