Exploring new sources of coffee resistance to leaf rust (Hemileia vastatrix)
PTDC/ASP-PLA/29779/2017.
Coffee leaf rust, caused by the biotrophic fungus Hemileia vastatrix (Hv), is the most important disease of Coffea arabica. This project aims to apply an integrative approach to explore a potential new source of coffee resistance to Hv, dissect host resistance mechanisms and provide tools for rapid and easy selection of resistant genotypes. The Kawisari hybrid (C. arabica x C. liberica) derivative (CIFC 644/18), recently used as a resistant donor in breeding programs in India, was selected to characterize its resistance by integrating cytology, next-generation sequencing by Illumina, and RT-qPCR gene expression analysis. Combining all data, we expect, as an ultimate goal, to identify biomarkers putatively linked to coffee resistance, which will be tested on other coffee genotypes with large spectra of resistance to Hv, as a future useful tool for marker-assisted selection. Sequence information will be made publicly available for future projects on coffee and other crops.
Principal investigator (PI) - Maria do Céu Silva
Co-principal investigator (Co-PI) - Dora Batista
Principal contractor:
Instituto Superior de Agronomia, Universidade de Lisboa
LEAF - Linking Landscape, Environment, Agriculture, and Food
Participating Institutions:
FCiências.ID / Faculdade de Ciências da Universidade de Lisboa,
BioISI | BioSystems & Integrative Sciences Institute
BIOPOLIS-CIBIO, Universidade do Porto
Research Centre in Biodiversity and Genetic Resources