Embedded Files
Click HERE to download the Certificate page need to be placed in the record
NEP Syllabus from 2023-24 onwards
MJD12 Practicals – AGRICULTURAL MICROBIOLOGY (3+1 credits)
The fluorescent green highlighted experiments are those we take up in this semester
1. Isolation of bacteria, fungi and actinobacteria from soils.
2. Isolation of nitrogen fixing bacteria from legume root nodules (Expt. 1; Dt.20-02-2026; Lect Notes; Video Lect)
- To know about the basics of the Rhizobium click HERE
- To know about the names of rhizobium that nodulate legumes click HERE
3. Enumeration of rhizosphere to non rhizosphere population of bacteria.
4. Isolation of antagonistic Pseudomonas from soil.
5. Microscopic observations of root colonization by VAM fungi.
6. Isolation of Azospirillum sp. from the roots of grasses (Expt. 2; Dt.06-03-2026; Lect Notes; Video Lect).
- To see the microscopic movement of Azospirillum click HERE
7. Isolation of phyllosphere microflora (Expt. 3; Dt.20-03-2026; Lect Notes; Video Lect).
- To know how PPFMs benefited the farming community in the Cauvery delta region in Tamil Nadu, India read HERE
8. Isolation of P solubilizing microorganisms.
9. Observation of Anabaena from Azolla plants (Expt. 4; Dt.03-04-2026; Lect Notes; Video Lect).
- A video in Tamil explaining the difference between the duckweed and the Azolla, to watch it click HERE
- Azolla and Anabaena image for record you can download by clicking HERE
10. Demonstration on different biofertilizers types, formulation and application methods.
11. Visit to biofertilizers and biopesticides unit to understand about the Unit operation procedures.
Reference:
Amaresan N., Patel P. and Amin D. (Eds.) (2022) Springer Protocols: Practical Handbook on Agricultural Microbiology. Springer. p.391
Additional informations:
For additional practical experiments details click HERE [Source: Under graduate course AGM 301 Soil and Applied Microbiology (2+1)]
Keep the following points in mind while preparing and submitting record for valuation
- Maintain a 80 pages unruled long size notebook as OBSERVATION NOTE
- Write in the RECORD NOTE BOOK similar to one you use in schools and should NOT write in loose sheets and bind.
- Fill the first paragraph of the Certificate page, paste in the record, click HERE to download the certificate page.
- Fill the Index page with the details of the Expt No., title, date and submit.
- Scientific names need to be underlined; check HERE to see how to write scientifc names.
- While writing experiment remember, diagrams (drawn with pencil) media composition (are need to be written in left side in pen) and the textual portions alone need to be written in the right side.
- While writing the experiment in right side pages of a record, dont forget to add in the top left side corner the experiment number and date.
- Cover the record with a brown cover and submit.
- If you plan to submit record late due to any personal reasons or issues, before submission date get permission from course teacher by filling the permission form.
(Note: Record lacking any one of the above points WILL NOT BE EVALAUTED/INTERNAL MARKS PROPORTIONATELY REDUCED)
The following are the medium compositions and the protocols commonly used in the class teaching
The following are the medium compositions and the protocols commonly used in the class teaching
1Stock Calculations for AMS medium.pdf2 Medium Compositions.pdf3Vss_Agrl Microbiol Practical final.docDocumentation efforts of 2023
Documentation efforts of 2023
Methylobacterium rhodesianum.pdfDocumentation efforts of 2017
Documentation efforts of 2017
Enterobacter cloacae subsp.pdfA presumptively identified culture in YEMA medium as Rhizobium based on the copious polysaccharides production, however, when its 16S rRNA gene was sequenced it was placed into Enterobacter spp. This strongly enforces the point that morphological identification cannot be used as SOLE CRITERION in bacterial identification.
Methylobacterium rhodesianum.pdfMethylobacterium strains.pptxMethylobacterium colonies dwelling in leaves were isolated in Petri plates employing Methanol or ammonium mineral salts (MMS/AMS) medium using leaf imprinting technique (Holland et al. 2000).
The second slide shows the phylogenetic position of the strain and the same was submitted in the GenBank and documented, to see the GenBank accession details click HERE.
Documentation Efforts of 2016
Documentation Efforts of 2016
The above plate shows the growth of Azospirillum in the N free malic acid medium.
The left hand side greenish yellow shows the acidic nature of the medium and in the right side you can see the change in the medium colour to blue after Azospirillum growth indicating pH change to alkaline.
Growth on Azospirillum in the N free semi solid medium showing the colour change after incubation
The pale yellowish colonies is a purified Azospirillum strain growth in the R2A agar
Azospirillum growth and colour change in the N free malic acid medium
Gummy colonies presumptively thought as Rhizobium
Documentation efforts of 2015
Documentation efforts of 2015
Azospirillum isolation
Rhizobium colonies showing the copious amount of polysaccharides secretions
AM isoalted from Castor roots.pptxMycorrhiza isolated from the castor roots are stained with tryphan blue and visualized, note the presence of fat globules and in certain images, the fine intricate arbuscules and the resting spore was also identified but vesicles are absent or difficult to identify.
Methylobacterium tarhaniae strain BMF145 16S ribosomal RNA gene, parti - Nucleotide - NCBI (2014).pdfThis one is a Methylobacterium spp. isolated from a Ficus religiosa plant in Tamil called as அரச மரம் (Arasa maram) grown on a wall cervices of Dept. of Microbiology.
The strain isolated in our class study is phylogenetically nearer to the one isolated from a dry environment, an arid soil
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