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NEP Syllabus from 2023-24 onwards
MJD12 Practicals – AGRICULTURAL MICROBIOLOGY (3+1 credits)
1. Isolation of bacteria, fungi and actinobacteria from soils.
2. Isolation of nitrogen fixing bacteria from legume root nodules (Expt. 1; Dt.20-02-2026; Lect Notes; Video Lect)
3. Enumeration of rhizosphere to non rhizosphere population of bacteria.
4. Isolation of antagonistic Pseudomonas from soil.
5. Microscopic observations of root colonization by VAM fungi.
6. Isolation of Azospirillum sp. from the roots of grasses (Expt. 2; Dt.06-03-2026; Lect Notes; Video Lect).
7. Isolation of phyllosphere microflora (Expt. 3; Dt.20-03-2026; Lect Notes; Video Lect).
8. Isolation of P solubilizing microorganisms.
9. Observation of Anabaena from Azolla plants (Expt. 4; Dt.03-04-2026; Lect Notes; Video Lect).
10. Demonstration on different biofertilizers types, formulation and application methods.
11. Visit to biofertilizers and biopesticides unit to understand about the Unit operation procedures.
Reference:
Amaresan N., Patel P. and Amin D. (Eds.) (2022) Springer Protocols: Practical Handbook on Agricultural Microbiology. Springer. p.391
Additional informations:
For additional practical experiments details click HERE [Source: Under graduate course AGM 301 Soil and Applied Microbiology (2+1)]
A presumptively identified culture in YEMA medium as Rhizobium based on the copious polysaccharides production, however, when its 16S rRNA gene was sequenced it was placed into Enterobacter spp. This strongly enforces the point that morphological identification cannot be used as SOLE CRITERION in bacterial identification.
Methylobacterium colonies dwelling in leaves were isolated in Petri plates employing Methanol or ammonium mineral salts (MMS/AMS) medium using leaf imprinting technique (Holland et al. 2000).
The second slide shows the phylogenetic position of the strain and the same was submitted in the GenBank and documented, to see the GenBank accession details click HERE.
The above plate shows the growth of Azospirillum in the N free malic acid medium.
The left hand side greenish yellow shows the acidic nature of the medium and in the right side you can see the change in the medium colour to blue after Azospirillum growth indicating pH change to alkaline.
Growth on Azospirillum in the N free semi solid medium showing the colour change after incubation
The pale yellowish colonies is a purified Azospirillum strain growth in the R2A agar
Azospirillum growth and colour change in the N free malic acid medium
Gummy colonies presumptively thought as Rhizobium
Azospirillum isolation
Rhizobium colonies showing the copious amount of polysaccharides secretions
Mycorrhiza isolated from the castor roots are stained with tryphan blue and visualized, note the presence of fat globules and in certain images, the fine intricate arbuscules and the resting spore was also identified but vesicles are absent or difficult to identify.
This one is a Methylobacterium spp. isolated from a Ficus religiosa plant in Tamil called as அரச மரம் (Arasa maram) grown on a wall cervices of Dept. of Microbiology.
The strain isolated in our class study is phylogenetically nearer to the one isolated from a dry environment, an arid soil