Practicals
Click HERE to download the Certificate page need to be placed in the record
CELL BIOLOGY University Practical examination is scheduled on 12-12-2024
MID1 Practicals – II: BIOMOLECULES & CELL BIOLOGY (1 credit)
Unit – VI 30 hours
Preparation of Molar, Normal solutions and buffers – Determination of pH using pH meter – Qualitative analysis of carbohydrates, protein and fats – Estimation of glucose by Benedict’s test – Estimation of ascorbic acid – Preparation of starch from potatoes, Understand about the cell types of prokaryotes and eukaryotes, by observing their structure, the cell division and function – Observing the internal structures of the cells, their importance and functioning.
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Experiments Details:
1. Study of plant and animal cell type’s basic structure using micrographs or model
2. Stomatal cell type in plant tissues
Methodology - Both peel & Varnish method
3. Blood as liquid tissue – demonstrating the different types of blood cells
To get correct results while performing the experiment, three steps are much important to know them click HERE
(I) Watch Preparation of blood smear (Video 1) and a staining model (NOT USED in our lab) of blood smear (Video 2)
(II) Click HERE amd HERE to see how the blood cells appear after Leishmann staining;
Dont call facuty to examine your slide until you see clear focus like the one shown HERE
AFTER PERFORMING EXPERIMENT WATCH THE PRESENCE OF FOLLOWING CELL TYPES : (i) RBC (ii) Neutrophil (iii) Eosinophil (iv) Lymphocytes (v) Monocytes (vi) Basophil
For drawing in the record or observation, check this video link
REMEMBER: Atleast 4 cell types need to be shown to faculty through microscope, draw the same in the course journal and get signed by facultyMORE INFORMATION: For a detailed information about the blood cells click HERE
4. Cytoplasmic streaming in eukaryotic cells.
To see the cytoplasmic streaming (cyclosis) in the Elodea plants click HERE; Inorder to obtain a data sheet on the observation of Elodea leaves click HERE
5. Mitosis demonstrated using onion root tip method
6. Study of bacterial cell structure, shape and arrangement using micrographs or model.
7. *Studying the different cellular organelles of the eukaryotic cells with animation and micrographs.
AFTER WATCHING THE ABOVE VIDEO YOU SHOULD BE FAMILIAR WITH FOLLOWING TERMS - Lipid raft, phospholipid bilayer, Cholesterol, Peripheral protein, integral membrane protein, G protein, Cytoskeleton (Actin filaments, Microtubules) Vesicle, Motor protein, Mitochondria, centrosomes with centrioles, Mitochondria, with centrosome and vesicle, Nuclear pores, Endoplasmic reticulum, Golgi body, exocytosis of proteins
REMEMBER : Draw diagram of the each structure/organelle listed above (in left hand side of record with pencil) and write a paragraph about that organelle in the right hand side of record.
CLEAR DIAGRAMS FOR RECORD - Integral and Peripheral proteins; Microfilaments and microtubules; Microtubule assembly with motor proteins; Vesicle; Mitochondria; Centrosome with centrioles; Nuclear pores; Endoplasmic reticulum; Golgi Apparatus; Exocytosis; Phospholipid bilayer with cholesterol
* This question may be asked in the examination as identification, so be familiar with the organelles of the cell.
8. Demonstration of quorum sensing in bacteria using animation or micrographs
Experiments going to be conducted (2024)
Experiments shown in turquoise blue indicate those that were undertaken in the present semester
Ex. No. 1 - Date 01-11-2024- Plant and animal cell structure using micrographs
Ex. No. 2 - Date 08-11-2024 - Observation of stomata cells in plant leaves
Ex. No. 3 - Date 29-11-2024 - Studying different cellular organelles of eukaryotes by animation
Ex. No. 4 - Date 06-12-2024 - Blood as liquid tissue - demonstration of different types of blood cells
Ex. No. 5.- Date 10-01-2024 - Demonstration of quorum sensing in bacteria using animation or micrographs
Ex. No. 6 - Date 26-10-2024 - Cytoplasmic streaming in eukaryotic cells
References
Shakya A., Shrivastava N. and Joshi S. (2020) Cell Biology Lab (BBCCL-104) Indira Gandhi National Open University, p. 54.
Keep the following points in mind while preparing and submitting record for valuation
Maintain a 80 pages unruled long size notebook as OBSERVATION NOTE
Write in the RECORD NOTE BOOK similar to one you use in schools and should NOT write in loose sheets and bind.
Fill the first paragraph of the certificate page, paste in the record, click HERE to download the certificate page.
Fill the index page with the details of the Expt No., title, date and submit.
Scientific names need to be underlined; check HERE to know how to write scientific names
While writing the experiment in right side pages of a record, dont forget to add in the top left side corner the experiment number and date.
While writing record remember that, diagrams (draw in pencil)/ media composition/ equations are need to be written in left side and
the textual portions alone need to be written in the right side.
Cover the record with a brown cover and submit correctly on the day course teacher insisting to submit.
If you plan to submit record late due to any personal reasons or issues, before submission date get permission from course teacher by filling the permission form
(Note: Record lacking any one of the above points WILL NOT BE EVALAUTED/INTERNAL MARKS PROPORTIONATELY REDUCED)
