Part 3: Extraction With An Aqueous Base

Testing the pH of the Aqueous Layer

Combine the aqueous layers in a single flask labeled E. Test the pH of solution E. Designate the organic layer as A2 and spot a sample in the third lane of the TLC plate.

This video shows a small flask with a colorless liquid inside. A gloved hand uses a tweezers to dip a piece of pH paper into the liquid and holds the pH paper up to the camera. The pH paper has turned yellow in color.

A photo of a piece of pH paper held by tweezers next to a color key. The color is yellow and correpsonds to pH 7.

A photo of a TLC plate illuminated under UV light with three spots visible on the baseline labeled A1, C, and A2.

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Extract the organic layer

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