Projects

Ongoing Research Projects:

• Cell culture, FACS, Coral organoid development, and establishing molecular and cellular studies

• Studying environmental effects such as pollutants in corals and in human insulin signaling

• Integration of mass spectrometry analysis and metabolomics with insulin signaling and cell differentiation studies

• Developing and deriving molecular similarities between humans and corals insulin signaling pathways

• Transfection of coral cells and fragments for knockdown/knockin

• Metabolic profiling of coral symbiont cells

Project: Metabolic Profiling of Rhodopsin Expressing Cell lines in Retinitis Pigmentosa

• Study metabolic effects of rhodopsin misfolding in retinitis pigmentosa in humans

• Cell culture of rhodopsin mutant cell lines, extraction, and GC/MS sample prep for metabolic profiling

• Protein quantification of rhodopsin expressions by SDS page and western blot

• Purification of 1D4-tagged rhodopsin in Sepharose affinity column chromatography

• Cell transfection, Protein extraction, computational and functional validation studies

• Protein characterization: Western blot, HPLC, ELISA, Dynamic light scattering (DLS), Crystallization

• Binding of Benzo(a)pyrene to rhodopsin

• Coral opsin transfection into mammalian cells for purification and crystallization

• Establish coral 2D cell culture and 3D organoid development

• Organoid characterization, microscopy

• Coral tissue characterization and histopathology

Past Projects:

Project: Laminin interactions with the islet in a 3d scaffold protect against cytokine-mediated β-cell death, Colorado School of Mines

• Studyied the effects of extracellular matrix (ECM) on the islets and its role in Beta-cell apoptosis

• Performed biomaterial-based ECM studies, polymer synthesis, and characterization using SEM, NMR, and FTIR2

• Performed cell culture, protein purification, and analysis using affinity purification, western blot, FRET, confocal imaging

• Isolated islets from mice and performed in vitro and in vivo animal studies

Project: Development of full-thickness skin equivalents: reconstruction of skin to treat Epidermolysis bullosa (EB) using human induced pluripotent stem cells (iPSCs), University of Colorado Denver

• Performed cell culture work such as generation of Induced pluripotent stem cells (iPSCs), reprogramming,

nucleofection, transfection, differentiation into skin cells

• Performed cell characterization and cell-based assays such as FACS, immunofluorescence, and confocal imaging

• Developed 3D skin models of the patients with inherited skin blistering disorders, performed an in-vitro analysis

of regenerated skin and in-vivo analysis by grafting on mice

• Optimized and established a standard operating procedure (SOPs), and managed large-scale experiments

Project: Demyelination in the Pediatric Spinal Cord Detected with Magnetization Transfer Imaging Ratio, Boston Children's Hospital

• Planned, developed, and implemented an image processing algorithm for Spinal Cord MRI data processing

(Image processing, Segmentation) in Pediatric Health Care using MATLAB as a tool

• Data acquisition, Analysis, Validation, and Interpretation of patient data

Laboratory skills: Organoid development, Mass spectrometry GC/MS, HPLC, Size exclusion chromatography, 3D skin

reconstruction, Animal surgery, Stem cell culture, IPSCs reprogramming and culture, Transfection, Microtome,

Immunohistochemical, Immunofluorescence staining, Confocal microscopy imaging, Scaffold fabrication, PCR, Western

Blot, DNA and RNA isolation, Flow cytometry-cell sorting (FACS), FRET, Biomaterial synthesis and functionalization-

NMR, SEM, Protein extraction, and purification, DLS, HPLC, ELISA, and Cystalization.

Application Development Tools: MATLAB, Metabolomics analysis, Statistics, Python, Image J, PyMol, Autodock Vina,

Science illustration, Intellectual Property management, Technology Licensing, Marketing.

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