Building on my previous research, I continued my investigations to ensure the purity of fungal strains, employing genetic methods such as metagenomics. My experimental approach involved fumigant bioassays, minimum inhibitory assays, and the thorough characterization of fungal strains. Metagenomics analyses were conducted on stored seeds, and I also examined biocontrol agents isolated from these seeds. Identification of compounds in essential oils was achieved using GC-MS, while TLC was utilized to observe Aflatoxin presence in stored seeds, all with a primary focus on mitigation of aflatoxin. Notably, I discovered and documented four novel essential oils for further analysis. This research contributes to the ongoing efforts to develop effective strategies for controlling aflatoxin contamination in stored agricultural products.
In my research, I conducted extensive sampling in warehouses, collecting samples from peanut, maize, and coffee seeds. Subsequently, I isolated fungal strains and identified them as Aspergillus through microscopic analysis. Further investigation, employing Thin-Layer Chromatography (TLC) techniques, revealed the presence of Aspergillus flavus and Aspergillus parasiticus based on the aflatoxin bands on silica plate. To mitigate the impact of these identified strains, I employed various approaches, including essential oils and biocontrol agents. The screening of essential oils was a key aspect of my work, and I utilized TLC, Microscopy and GC-MS techniques during the research period. This comprehensive study contributes to understanding and addressing fungal contamination in stored agricultural products, offering insights into potential control strategies.
In my role, I was focused on researching and developing solutions for air and water pollution control, with a specific emphasis on Chlorella sorokiniana. I managed the laboratory operations, overseeing experiments related to algal photobioreactors, algae raceway ponds, and utilizing analytical techniques such as Soxhlet, HPLC, and GC-MS. Additionally, I have guided two dissertation students, imparting my knowledge and expertise in the field. My work was aimed to contribute to advancements in sustainable technologies for environmental remediation, particularly in the context of algae-based solutions.
In my role as an assistant professor, I conducted regular classes for both M.Sc. Botany and B.Sc. students, focusing on delivering comprehensive lectures and engaging course material. Additionally, I facilitated practical sessions, providing hands-on experience to enhance students' understanding of theoretical concepts. Through effective teaching methods, I aimed to foster a conducive learning environment and contribute to the academic development of students in the field of botany.