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Forced-Swim stress modulates the hepatic gene expression, induces liver damage, and subsequently promotes the progression of type 2 diabetes in mice
Abeer Asif
Research has identified that stress significantly contributes to liver diseases like T2DM (Type 2 Diabetes Mellitus). Our study utilizes forced swim stress to induce emotional stress in C57 black male mice over a period of 14 days, 15 minutes per day, and have found interesting results. Biochemical test reports showed a significant increase in ALP, ALT, hemoglobin, and MCH values in experimental and control mice when compared to control mice showing a possible trend toward liver damage. This is further reinforced with visible data through H & E staining which shows ballooning degeneration, swelling in hepatocytes, and nuclear enlargement in experimentally stressed mice livers. To obtain molecular insight into this, we utilized gene analysis through conventional PCR, densitometry, and real-time PCR. We analyzed the expression of targeted genes and found an up-regulation of Crp, Cyp2e1, and Irs-2. Irs-2 was revealed to have an initial protective effect against liver damage. With early detection through potential biomarkers like Crp, we can be diagnosed ahead of time and implement the lifestyle conditions needed for the reversal of diabetes.
Links
The Comparison of Mutational Progression in SARS-CoV-2: A Short Updated Overview.
Journal of Molecular Pathology.
DOI: https://doi.org/10.3390/jmp3040018
Forced-Swim stress modulates the hepatic gene expression, induces liver damage, and subsequently promotes the progression of type 2 diabetes in mice.
Journal of Oxidative Medicine and Cellular Longevity. DOI:
Posters
'Customized DNA Assembly for Genetic Screening and Gene Synthesis'-Business Idea Competition in Life Sciences- 11th Mar 2019'Cost-effective multiple SNP assembly for rapid genetic analysis'- A short course in CancerBio-12th to 14th Feb 2019
'A Novel Diagnostic Tool for SNP based Genetic Analysis'- Expo- 11th Feb 2019
''Cost-effective Assembly of Metastatic Breast Cancer Risk Associated SNPs'-ICNS-4th to 5th Dec 2018
Cost effective assembly of metastatic breast cancer risk associated SNPs
Nimra KhurramCost effective assembly of metastatic breast cancer risk associated SNPs
Polymerase chain reaction (PCR) is famous for its ability to exponentially amplify specific regions of the DNA molecule. Assembly PCR is a process that precisely joins two or more PCR products with the help of tailor made primers. The customized primers have complementary extensions which enables PCR products of first round to be assembled in subsequent cycles. In application, different and distant genomic regions of the DNA could be assembled in one PCR product. This technique would help in screening and diagnosis of diseases related to multiple single nucleotide polymorphs (SNPs). A multi-factorial disease such as breast cancer is found to be associated with many SNPs present on different loci. We successfully assembled (in pairs) four metastatic breast cancer related SNPs to confirm the use of our technique as an application to molecular diagnostics. The tailored primers generated differently sized initial PCR products with fifteen base pair overlapping regions inclusive of EcoRI sites. The overlapping sequences enabled self-assembly of initial PCR products to generate a seamless construct containing genetic information of different chromosomal locations. The assembly products were analyzed for their integrity using EcoRI based restriction analysis followed by DNA sequencing for SNP analysis.Key words: assembly PCR, metastasis, SNP
Evaluation of anti-metastatic potential of long non-coding RNA MALAT-1 in breast cancer
Aliza QaiserEvaluation of anti-metastatic potential of long non-coding RNA MALAT-1 in breast cancer
Expression profiling of stress-associated genes in metastatic breast cancer patients
Tahreem FiazExpression profiling of stress-associated genes in metastatic breast cancer patients
Expression analysis of DNA methyltransferases (DNMTs) in patients with breast cancer under chemotherapeutic stress
Anam Rafique Expression analysis of DNA methyltransferases (DNMTs) in patients with breast cancer under chemotherapeutic stress
Combinatorial effect of HOXA10 and p53 in breast cancer progression and metastasis
Sher AzamCombinatorial effect of HOXA10 and p53 in breast cancer progression and metastasis
Hina Saif
The research project considers the Integrity of the CFTR gene of the Individuals suffering from Congenital Bilateral Absence of Vas Deference (CBAVD). CFTR gene plays a significant role in the development of both CBAVD and Cystic fibrosis. And since, CBAVD is strongly linked with Cystic fibrosis, therefore their research diagnosis is sometimes coupled. CBAVD is a genetic condition that is present since birth and due to which male genital track (Vas Deference) fails to develop thus leading to male infertility. This study is particularly designed to study the promoter region of the CFTR gene and investigate its nature in the patients with CBAVD and how it may affect the development of the disease. Since, the Promoter regions play an essential part in gene expression and contribute towards creating genetic variations, finding out the integrity of the promoter region of the CFTR gene of the patients with CBAVD could assist in the better understanding of this genetic condition.
Tumor Invasion and Metastasis due to miR-135a in a Cohort of Breast Cancer Patients
Maham Rashid Tumor Invasion and Metastasis due to miR-135a in a Cohort of Breast Cancer Patients
Steroid hormone receptors have been shown to play significant roles in breast carcinogenesis. Both progesterone receptors and estrogen receptors have been detected in most of the breast carcinomas. Recent studies have shown that androgen receptors (ARs) also play important roles in most breast tumors. Micro RNAs have been shown to regulate these hormone receptors. This project aims to identify the role of particular miRNA in association with the androgen receptors in breast cancer. Such identification of prognostic and predictive biomarkers can promote individuality of breast cancer management and more effective administration of chemotherapeutics.
Targeted assembly of distant disease related multiple SNPs
Aqsa Aslam Targeted assembly of distant disease related multiple SNPs
Single nucleotide polymorphisms (SNPs) are found to be related with genetic basis of different diseases. Cumulative effect of multiple SNPs located on different genes can lead to the onset of cancer. Traditionally multiple SNPs are screened through multiple PCR reactions followed by sequencing. We designed a rapid and cost-effective targeted SNP assembly (TSA) technique which can connect these target SNPs locations to generate one single PCR product for sequencing.in this study we successfully assembled four SNPs locations related to breast Cancer in single PCR product by TSA.
Mutational analysis of CFTR in patients with congenital bilateral absence of vas deferens
Hafsa IftikharMutational analysis of CFTR in patients with congenital bilateral absence of vas deferens
Role of PTEN in PI3/AKT signaling pathway and breast cancer tumorigenesis
KhushBakht Role of PTEN in PI3/AKT signaling pathway and breast cancer tumorigenesis
Breast carcinoma is the most common type of malignancy and second leading cause of death in females all over the world. Mutations in various tumor suppressor genes cause dysregulation of critical signaling pathways and result in the loss of cell’s ability to control its key function in breast epithelial cells. One well know gene is PTEN which antagonize PI3K/AKT signaling pathway by dephosphorylating phosphatidylinositol‑3,4,5‑trisphosphate (PIP3) which is strong activator of AKT. We are analyzing integrity of candidate gene in Pakistani breast cancer patients and the effect of mutations in PTEN on the expression of downstream signaling pathways to establish a link to understand its role in tumorigenesis.
Functional Study of tumor suppresor HOXA10 and its role in p53 regulation in breast cancer
Aizaz Izzat Functional Study of tumor suppresor HOXA10 and its role in p53 regulation in breast cancer
The HOXA10 gene is an emerging tumor suppressor gene that has found to be silenced in hereditary breast cancer. It has been shown to induce transcriptional activation of p21, which causes cell cycle arrest in U937 cells and it has also shown to play a role in p53 regulation in breast cancer cells. However the mechanism of said process is still unclear. The aim of this study is to identify germ line mutations in the promoter region (located 5.3 – 6.1 KB before the fisrt start codon of HOXA10) of breast cancer patients that may affect its relationship with p53 and so play a role in breast cancer regulation.
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