Regulation of Genes

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Dr. Muhammad Mustafa

Member Single National Curriculum Committee (SNCC)

Federal Ministry of Education and Professional Training

EmailAssistant Professor School of LIfe SciencesForman Christian College (A Chartered University) Lahore Pakistan
FC College University
Yonsei University

Research Interest

We are investigating the role of developmental proteins (e.g., HOXA10) in regulation of tumor suppressors (e.g. p53) to identify the molecular mechanisms fueling metastasis in breast cancer. The study reveals novel therapeutic targets for drug industry to fight against the leading cause of cancer deaths in Pakistan. Epigenetic silencing of tumor suppressors by chromatin remodeling proteins like CTCF is an active area to find potential locations for gene therapy in cancers. In another front we have established a functional relationship of CFTR gene with male infertility disease CBAVD and working to unveil the signaling cascades involved in disease onset.

Featured Image

CTCF knock down dependent expression of p53 results in cancer cell death by isolating cancer cell from the surrounding with decreased ITGB4 expression

Theme (Mustafa, J Cancer 2017; 8(11):2124-2131)

Figure S6. Expression and localization of ITGB4 in MCF-7 cells. (A) mRNA expression level of ITGB4 and CTCF in control non-specific (Control-NS) and CTCF-knockdown (CTCF-KD) cells. (B) Confocal microscopy images showing ITGB4 and p53 in Control-NS and CTCF-KD cells. Antibodies against p53 (sc-126; Santa Cruz,), ITGB4 (ab133682; Abcam) and the corresponding secondary antibodies (anti-rabbit IgG Alexa Flour 594 and anti-goat IgG Alexa Flour 488; Invitrogen) were used for detection. Cells were counterstained with DAPI (Invitrogen) and analyzed using a Zeiss LSM700 confocal microscope.Zoomed images of square boxes in c and g are shown in i-l and m-p, respectively.

Our Contribution

CTCF is a Transcriptional inhibitor of HOXA10 as well as p53, it binds directly to the promoter region of these two tumor suppressors, interferes with RNA pol II and supports the recruitment of inactive epigenetic markers at the promoter region. Cancer cell use higher levels of CTCF to turn off these critical tumor suppressors to promote their survival and proliferation.


CRISPR/Cas9: targeted genome editing for the treatment of hereditary hearing loss

(J Appl Genetics, 2020.

News and Media Publications

Unlocking Future Of Breast Cancer Detection: The AI Revolution (Technology Times, 6 October,2023)

Food supplements may help prevent cancer by regulating gene expression (Technology Times, 14 September, 2023)

AI and the Future of Prosthetics (Scientia Magazine 22, June 2023)

Building Blocks Of Life: Cells And Their Specialization In Human Organs (Technology Times Pakistan 21, June 2023)

AI and Neurobiology: Understanding the Brain through Computational Models (Scientia Magazine 19, June 2023)

Intersex (Khawajasira) By Choice Or Chance? (Technology Times Pakistan 10, June 2023)

H5N1: Understanding Deadly Avian Flu (Technology Times Pakistan 6, June 2023)

Better Breast Cancer Care for All (The Pathologist 8, May 2023)

Role of genetics in infectious diseases spread, genetical evolution (Scientia Magazine 26, April 2023)

Successful cure for HIV with Stem Cell Transplantation (Scientia Magazine 15, April 2023)

Why we are lacking behind in Science and Technology (Express News TV- 8, Nov 2022)

Broken-Heart Syndrome Or Stress-Induced Cardiomyopathy? (Sep 30, 2022 Technology Times)

Emotional Stress and Cardiac Complication  (Sep 30, 2022 Technology Times)

Education ministry sets up committee to oversee curriculum standards (Jan 23, 2021 Dawn)

Coronavirus fidelity: Carry logical fears (February 09, 2020 Technology times)

The codes of generation gap (February 03, 2020 THE NEWS)

The Single Specie Track of Human Evolution (January 09, 2020 Spectra Magazine)

Neonatal Care of preterm babies with artificial placenta (October 23, 2019 Technology times)

Neuroprosthetics: a future for human and machine (September 24, 2019 Technology times)

Emotional Stress: Fueling Cancer Metastasis (September 4, 2019 Spectra Magazine)

The biological secret of aging (September 1, 2019 THE NEWS)

Phage game: allies of the post antibiotic era (August 19, 2019 Technology times)

Lactose Intolerance- a normal abnormality (August 9, 2019 Technology times)

3D bio-printing of organs: science-fiction or reality? (July 11, 2019 Technology times)

Breast cancer dilemma; if you can't complete it, manage it (July 10, 2019 Technology times)

Research Conducted at Yonsei University Has Updated Our Knowledge about Breast Cancer Women's Health Weekly December 2015

Dr. Mustafa at Researchgate

Dr. Mustafa at Google Scholar



Forman Christian College University

FC College University Faculty

Yonsei University

HEC Scholarship for Higher Studies

Study in Korea


Department of Biological Sciences

S435 Armacost Science Building

Forman Christian College (A Chartered University)

Ferozepur Road, Lahore 54600


+92 (42) 99231581-8 Extension 524