Final Design

• In order to allow the user to place and remove petri dishes into and out of the device with no dissambely required, the motors were spaced farther apart.

• Spring loaded petri dish clamps to keep the petri dish completely stationary while being stretched.

• Petri-dishes made out of high temperature resistant plastic, to withstand Autoclave temperatures

• Spring loaded rotational stepper motor

Revisions made to the GUI for final design:

• • Constant feedback of what program is doing via “Status Text Box”

  • Program forces user to go through correct sequence of steps by disabling buttons if not possible to perform the button’s given function

  • Live image and live updating filtered image of microscope view with crosshair overlay for ease of object of interest designation

    • • Program automatically detects closest object to crosshair and designates it as object of interest. User not required to perfectly align object with crosshairs

      • Marks object of interest with a green asterisk to allow user to affirm as the object of interest

      • Tells user via status box if:

        • • No objects detected

          • Not enough objects detected (a second object must be detected for calculating percent stretch)

          • If second reference object is too close to edge of image frame and thereby in danger of being lost during stretching

  • Image Filtering parameters adjustment sliders: User can easily change image settings to adjust for given room lighting using sliders

  • Errors for if object lost during stretching

  • Before and after stretch snapshots of raw and filtered image of microscope view

  • Tab delimited summary of stretching sequence output to text file on desktop called “Stretch_Summary”

  • 6/5: Repeat last Stretch option – For repeating a controlled stretch without image feedback (i.e. in the dark)

  • Easy to read and understand GUI layout