MICROBIAL METAGENOMICS LABORATORY


Assess knowledge of the students regarding unix and command-line

It is very important to assess the knowledge of the students regarding the use of UNIX and the command-line interface in order eventually to form a couple of students having complementary abilities that can work on the data analysis.


FIRST/SECON DAY

General description on the unix command line

Before starting it will be very useful to have a general description regarding unix command line with examples; this can be skipped by students already at agood level in unix command line use.


First resource - a movie regarding command-line in unix

C-DEBIEBICSBEACON_Introduction_to_Bioinformatics_for_Metagenomics/Day 1 - Morning Part 1 Introductions and Introductions to Unix.mp4


Second resource - a movie about a simple analysis in QIIME2

Video with demo regarding basic usage of QIIME2


Let’s start!

First part will be based on QIIME2 tutorials already available here


These tutorials include some questions that students will answer during the practical part.


Overview of QIIME2 plugins tutorial.

This link is very useful because it describes the workflow of QIIME2, which is important in order not to be lost during the analysis and to keep track of the file names.

First experiment - moving pictures tutorial


Second experiment - Fecal microbiota transplant (FMT) study: an exercise


Third experiment - “Atacama soil microbiome” tutorial


Fourth experiment - Parkinson’s Mouse Tutorial