WandaWorks
This manual is meant to complement your training and is not intended to replace on-the-job training.
WPC Activity Associated
Training Contact(s)
Emory Chan
emchan@lbl.gov
67-4114
x7373
Last Updated
05/04/2015
OPERATING PROCEDURE
1. Installing WANDAworks on your computer
1.1. Install Igor Pro 6.0 or later on your computer
1.2. In Mac OS X, go to the /Users/<username>/Wavemetrics/Igor Pro 6 User Files
1.2.1. Copy “Boot WANDAworks.ipf” to the Igor Procedures folder
1.2.2. Copy the “LBL procedures” to the “User Procedures” folder
1.3. In Windows, follow the above instructions for the analogous Igor Procedures / User Procedures folders.
2. Loading WANDAworks
2.1. Open Igor Pro (6.0 or later).
2.2. Select “Menu > Macros > Load WANDAworks”
2.2.1. If you do not see this option in your Macros menu, you need to install WandaWorks or verify that it has been installed properly (see section ?)
2.2.2. If you have loaded WandaWorks correctly, you should see a “WANDAwork” menu visible in your menu bar.
3. Importing data exported from Symyx PolyView into WANDAworks
3.1. Select “Menu > WANDAworks > Import Symyx data” to open up the window, “WANDAworks Import Data.”
3.2. Click the “Select Data Path” button at the top to choose the folder to import from.
3.3. From the “List of Available Files” window, select the files that you want to import. You can shift-click to select multiple files, or use the “De/Select All” buttons.
3.4. At the bottom of the screen, you can change the destination data folder for the imported data, as well as the names for the X (wavelength) and Y (intensity) waves. Normally, the data folder “root:SpectralData”
3.5. Click the “Import” button at the bottom right corner to import the selected data files.
4. Configuring data for graphing
4.1. When you imported spectra into Igor, it generated one “wavelength_XXXX_X_X”’ wave per spectrum. HOWEVER, the graphing procedures require a single x wave named “wavelength” (exact spelling). Since plate reader data almost always uses the same wavelength values, you can delete all the wavelength waves but one.
4.2. Select “Menu > Data > Data Browser” to open the Data Browser window.
4.3. Click the Preferences button, and select Sort objects by name.
4.4. Back in the Data Browser, delete all but one of the wavelength_XXXX_X_X waves.
4.5. Rename the remaining wavelength wave to the exact name “wavelength”.
5. Graphing data
5.1. Select “Menu > WANDAworks > Plot and Analyze Spectra”
5.2. In the Peak Analysis window, select the desired waves to plot, then click “Graph selected spectra.”
5.3. You can change the well periodicity (e.g.6 for 6 aliquots per reactor) and then slide the “Element” and “Series” offset sliders to change the offsets of the plots.
6. Peak analysis
6.1. In the same “Peak Analysis” windows as above, select the spectrum type.
6.2. Set the range start/end values that describe the wavelength range over which peak finding will occur.
6.3. Set the baseline start/end values that describe the wavelength range over which baseline correction will occur. The baseline correction subtracts a constant baseline value equal to the average of the y values over that wavelength range.
6.4. Set the Fractional threshold value. The peak finding algorithm will not report peaks that have intensities less than (fractional threshold)x(maximum baselined intensity of spectrum)
6.5. In the bottom right corner, set the wavelengths for which you want the intensities exported.
6.6. Click the “Analyze selected spectra” button to generate a table of spectral data. You can save this table in Igor or text format by making the table window the top window and going to File > Save Table As…