Simulated microgravity was generated using a clinostat-based RPM system.  Cancer cell spheroids cultured under low and high RPM conditions showed altered morphology and increased compactness compared to static culture. Quantitative analysis revealed changes in spheroid size and shape. Cell viability and live/dead staining results indicated that simulated microgravity affects cell survival in a condition-dependent manner.

In order to compare existing random algorithms in the clinostat environment and to create an algorithm that can create an optimal simulated micro-gravity environment, three rotation conditions are set and compared to evaluate the effect of the periodicity of the two-axis rotation signal.