Protocols

General Preparation

How much K2EDTA is in the BD Microtainer® Tubes?

There is enough K2EDTA to anticoagulate blood volume from 250-500 μL. The average amount of K2EDTA in the tube is 1.0 mg. 

Obtain a 1cc insulin syringe (with needle attached) and coat with EDTA by pulling 10mM EDTA solution through the entire syringe. Leave approximately 25ï?­l 10mM EDTA in the syringe.

Recipes for Regular buffers