Publications
Publications (M.N. Yousaf* denotes independent publications as a Faculty since 2005)
*83. Bio-Orthogonal Mediated Nucleic Acid Transfection of Cells via Cell Surface Engineering. Paul J. O’Brien, Sina Elahipanah, Dmitry Rogozhnikov, and Muhammad N. Yousaf*. ACS Central Science 2017 . ASAP. DOI:10.1021/acscentsci.7b00132
Recent C&E News highlight on the company (www.OrganoLinX.com) and publication:
*82. General Dialdehyde Click Chemistry for Amine Bioconjugation. S. Elahipanah, P. J. O'Brien, D. Rogozhnikov, M. N. Yousaf Bioconjugate Chem. 2017 . ASAP. DOI: 10.1021/acs.bioconjchem.7b00106
*81. Scaffold Free Bio-orthogonal Assembly of 3-Dimensional Cardiac Tissue via Cell Surface Engineering. Dmitry Rogozhnikov, Paul J. O’Brien, Sina Elahipanah & Muhammad N. Yousaf* Nature Scientific Reports 2016 In press.
*80. Revising the Fluid Mosaic Model for Cell Membranes. M.N. Yousaf*. Perspective Review. In press.
*79. 3 Dimensional Control over cell to cell interactions by engineered cell surfaces. P.J. O'Brien, W. Luo, M.N. Yousaf*. Methods in Molecular Biology. Book Chapter. in press.
*78. Generation of a Scaffold-Free Three-Dimensional Liver Tissue via a Rapid Cell-to-Cell Click Assembly Process. Dmitry Rogozhnikov,† Wei Luo,† Sina Elahipanah,† Paul J. O’Brien,† and Muhammad N. Yousaf*,†,‡ Bioconjugate Chem. 2016. ASAP. DOI: 10.1021/acs.bioconjchem.6b00187
*77. Rewiring Gram-Negative Bacteria Cell Surfaces with Bio-Orthogonal Chemistry via Liposome Fusion. Sina Elahipanah†, Parham Radmanesh†, Wei Luo†, Paul J. O’Brien†, Dmitry Rogozhnikov†, and Muhammad N. Yousaf*†‡. Bioconjugate Chem. 2016. 27, 1082-1089.
*76. Spheroid and Tissue Assembly via Click Chemistry in Microfluidic Flow. O'Brien, P.J., W. Luo, D. Rogozhnikov, J. Chen, M.N. Yousaf*. Bioconjugate Chem. 2015. 26, 1939–1949 DOI: 10.1021/acs.bioconjchem.5b00376.
*75. A Dual Receptor and Reporter for Multi-Modal Cell Surface Engineering. W. Luo, N. Westcott, D. Dutta, A. Pulsipher, D. Rogozhnikov, J. Chen, M.N. Yousaf* ACS Chem. Biol. 2015. 10, 2219–2226. DOI: 10.1021/acschembio.5b00137
*74. Developing chemoselective and biodegradable polyester elastomers for bio-scaffold application. D.G. Barrett, W. Luo, M.N. Yousaf*. J. Mat. Chem. B. RSC. 2015. 3, 1405-1414
*73. A Molecular Smart Surface for Spatio-Temporal Studies of Cell Mobility. E.J. Lee, E.W.L. Chan, W. Luo, M. N. Yousaf*. PLOS ONE 2015. in press. http://dx.doi.org/10.1371/journal.pone.0118126
*72. In Situ Modulation of Cell Behavior via Smart Dual Ligand Surfaces. A. Pulsipher, D. Dutta, W. Luo, M. N. Yousaf*. Langmuir 2014 30, 13656–13666
*71. Remote Control of Tissue Interactions via Engineered Photo-switchable Cell Surfaces. W. Luo, A. Pulsipher, D. Dutta, B. M. Lamb, M. N. Yousaf*. Nature Scientific Reports 2014 in press. 6313 (2014) doi:10.1038/srep06313
*70. Controlling cell behavior with peptide nano-patterns. N. P. Westcott, W. Luo, M. N. Yousaf*. Journal of Colloid and Interface Science, 430, 2014, Pages 207-213
*69. Dynamic 3D cell culture via a chemoselective photoactuated ligand. N. P. Westcott, W. Luo, J. Goldstein, M. N. Yousaf*. Biointerphases 9, 031005 (2014); http://dx.doi.org/10.1116/1.4881035
Highlighted in Association of the Vacuum Society News.
*68. PI3 kinase enzymology on fluid lipid bilayers. D. Dutta, A. Pulsipher, W. Luo, M. N. Yousaf*. Analyst, 2014, Advance Article. DOI: 10.1039/C4AN00998C
*67. Cell division orientation on biospecific peptide gradients. B.M. Lamb, W. Luo, S. Nagdas, M.N. Yousaf*. ACS Appl. Mater. Interf. 2014, 6, 11523-11528.
*66. Cell surface engineering by a conjugation and release approach based on the formation and cleavage of oxime linkages upon mild electrochemical oxidation and reduction. A. Pulsipher, D. Dutta, W. Luo, M.N. Yousaf*. Angew. Chem. Int. Ed. 2014, 53, 9487-9492.
*65. Ligand slope, density and affinity direct cell polarity and migration on molecular gradient surfaces. E-J. Lee, E.W.L. Chan, W. Luo, M.N. Yousaf*. RSC Advances 2014, 4, 31581-31588.
*64. A General Chemoselective and Redox Responsive Ligation and Release Strategy. S. Park, N. P. Westcott, W. Luo, B. M. Lamb, M.N. Yousaf*. Bioconjugate Chemistry, 2014. 25, 543-551.
Engineering Cell Surfaces via Liposome Fusion. D. Dutta, A. Pulsipher, W. Luo, H. Mak, M.N. Yousaf*. Bioconjugate Chemistry. 2011, 22, 2423-2433.
Highlighted in C&E News 2011, 49, 36. Highlighted in NewsRx Drug Delivery News (01/12)
Biomolecular modification of carbon nanotubes for studies of cell adhesion and migration. W. Luo, M.N. Yousaf. Nanotechnology. 2011. In press. (Cover Article) (Special Issue on Bioengineering Nanotechnology)
Highlighted in Global Medical Discovery (02/12). Highlighted in Vertical News (Nanotechnology) (02/12)
Tissue Morphing Control on Dynamic Gradient Surfaces. W. Luo, M.N. Yousaf*. J. Am. Chem. Soc. 2011, 133, 10780-10783.
Highlighted in C&E News 2011, 29, 27.
A Redox Switchable Surface for Controlling Peptide Structure. B. M. Lamb, M.N. Yousaf*. J. Am. Chem. Soc. 2011, 133, 8870-8873.
Synthetic Chemoselective Rewiring of Cell Surfaces: Generation of Three-Dimensional Tissue Structures. D. Dutta, A. Pulsipher, W. Luo, M. N. Yousaf*. J. Am. Chem. Soc. 2011, 133, 8704-8713.
Highlighted in Science 2011, 332, 1011-1012.
A Renewable, Chemoselective, and Quantitative Ligand Density Spot Microarray for the Study of High-Throughput Biospecific Interactions. A. Pulsipher and M.N. Yousaf*. Chem. Comm. 2011. (Special Issue on Emerging Investigators) 47, 523-525
Developing A Self-Assembled Monolayer Microarray to Study Stem Cell Differentiation. W. Luo, M.N. Yousaf*, J. Coll. Int. Sci. 2011. 360, 325-330. (Cover Article)
One-Step Syntheses of Photocurable Polyesters Based on a Renewable Resource. D. Barrett, T. Merkel, C. Luft, M.N. Yousaf*. Macromolecules. 2010, 43 ,9660–9667
Thermosets Synthesized by Thermal Polyesterification for Tissue Engineering Applications. D.G. Barrett, M.N. Yousaf*. Soft Matter (Special Issue on Tissue Engineering) 2010, 6, 5026 – 5036.
Microfluidics for Molecular Interaction Assays on Surfaces. B.M. Lamb and M.N. Yousaf*. Methods in Molecular Biology (Chemical Genomics and Proteomics). Book Chapter. Editor. E. Zanders. 2010. in press.
Fabrication of Dynamic Self-Assembled Monolayers for Cell Migration and Adhesion Studies. N. P. Westcott and M.N. Yousaf*. Methods in Molecular Biology (Bioconjugation Protocols). Book Chapter. Editor. S. Mark. 2010. in press.
Self-Assembled Monolayers as Dynamic Model Substrates for Cell Biology. Pulsipher, M. N. Yousaf. *Advances in Polymer Sciences (Bioactive Surfaces). Book Chapter. Editors. Borner, Lutz. 2010. 1-32, DOI: 10.1007/12_2010_87
Microfluidic Permeation Printing of Self-Assembled Monolayer Gradients on Surfaces for Chemoselective Ligand Immobilization Applied to Cell Adhesion and Polarization B.M. Lamb and M.N. Yousaf*. Langmuir.2010, 26, 12817-12823.
Selective Tethering of Ligands and Proteins to a Microfluidic Patterned Electroactive Fluid Lipid Bilayer Array. D. Dutta, A. Pulsipher and M.N. Yousaf*. Langmuir, 2010. 26, 9835-9841.
Role of Surface Chemistry and Topology of Chemoselectively Tailored Embossed Films on Shear Adhesion. D. G. Barrett and M.N. Yousaf*. J. Mat. Chemistry. 2010, 20, 2813-2819.
Tandem Surface Microfluidic Lithography and Activation to Generate Patch Pattern Biospecific Ligand and Cell Arrays. A. Pulsipher and M. N. Yousaf*. Langmuir, 2010, 26, 4130-4135.
Aliphatic Polyester Elastomers Derived from Erythritol and α,ω-Diacids. D. G. Barrett and M. N. Yousaf*,Polymer Chemistry 2010, 1, 296-302.
Surface Chemistry and Cell Biological Tools for the Analysis of Cell Adhesion and Cell Migration. A. Pulsipher, M.N. Yousaf*. ChemBioChem 2010, 11, 745-753. (Cover Article)
Tailored Electroactive and Quantitative Ligand Density Microarrays Applied to Stem Cell Differentiation. W. Luo, E.W.L. Chan and M.N. Yousaf*. J. Am. Chem. Soc. 2010, 132, 2614–2621.
Highlighted in J. Am. Chem. Soc. 2011, 133, 167-169. (JACS SELECTS #11)
Design and Applications of Biodegradable Polyester Tissue Scaffolds Based on Endogenous Monomers Found in Human Metabolism. D. G. Barrett, M. N. Yousaf*. Molecules 2009, 14, 4022-4050
Patterned Hybrid Nanohole Array Surfaces for Cell Adhesion and Migration. N. P. Westcott, Y. Lou, J. F. Muth and M. N. Yousaf*. Langmuir 2009, 25, 11236–11238.
Spatio-Temporal Control of Cell-Cell Interactions on Surfaces. E.J. Lee, E.W.L. Chan, M.N. Yousaf*.ChemBioChem 2009, 10, 1648-1653.
Chemoselective Ligand Patterning of Electroactive Surfaces using Microfluidics. N. P. Westcott, M. N. Yousaf*. Electrophoresis 2009, 30, 3381-3385. (Cover Article)
Electrochemical and Chemical Microfluidic Gold Etching to Generate Patterned and Gradient Substrates for Cell Adhesion and Cell Migration. N. P. Westcott, B.M. Lamb, M.N. Yousaf*. Analytical Chem. 2009. 81, 3297–3303.
Rapid In-Situ Generation of Two Patterned Chemoselective Orthogonal Surface Chemistries from a Single Hydroxy-Terminated Surface Using Controlled Microfluidic Oxidation. A. Pulsipher, N.P. Westcott, W. Luo, M.N. Yousaf*. J. Am. Chem. Soc. 2009. 131, 7626–7632.
Model Systems for Cell Mobility. M.N. Yousaf*. Curr. Opin. Chem. Bio. 2009. 13, 697-704.
Tailored Electroactive Nanorods for Biospecific Cell Adhesion and Differentiation. W. Luo, M.N. Yousaf*. Chem. Comm. 2009, 10, 1237-1239.
Rapid Microfluidic Generation of Patterned Aldehydes from Hydroxy Terminated SAMs for Ligand and Cell Immobilization on Optically Transparent Indium Tin Oxide (ITO) Surfaces. A. Puslipher, N.P. Westcott, W. Luo, M.N. Yousaf*. Adv. Materials. 2009, 21, 3082-3086.
Total Internal Reflection Fluorescence Microscopy of Cell Adhesion on Patterned Self-Assembled Monolayers on Gold. D. Hoover, E.J. Lee, M.N. Yousaf*. Langmuir 2009, 25, 2563-2566.
Geometric Control of Stem Cell Differentiation Rate on Surfaces. W. Luo, S. Jones, and M. N. Yousaf*. Langmuir 2008, 24, 1229-1233.
An Electroactive Catalytic Dynamic Substrate that Immobilizes and Releases Patterned Ligands, Proteins and Cells. E.W.L. Chan, S. Park, M.N. Yousaf *. Angew. Chem. Int. Ed. 2008, 47, 6267-6271.
Renewable and Optically Transparent Electroactive Indium Tin Oxide (ITO) Surfaces for Chemoselective Ligand Immobilization and Biospecific Cell Adhesion. W. Luo, N. P. Westcott, A. Pulsipher, and M. N. Yousaf*.Langmuir 2008, 24, 13096-13101.
Poly(triol-α-ketoglutarate) as Biodegradable, Chemoselective, and Mechanically Tunable Elastomers. D.G. Barrett and M.N. Yousaf*. Macromolecules 2008, 41, 6347-6352.
Microfluidic Lithography to Create Dynamic Gradient SAM Surfaces for Directed Cell Migration. B. M. Lamb, N. P. Westcott, M. N. Yousaf*. ChemBioChem 2008, 9, 2628-2632.
Expedient Generation of Patterned Surface Aldehydes by Microfluidic Oxidation for Chemoselective Immobilization of Ligands and Cells. N. P. Westcott, A. Pulsipher, B. M. Lamb, M. N. Yousaf*. Langmuir 2008, 24, 9237-9240.
Asymmetric Peptide Nanoarray Surfaces for Studies of Single Cell Polarization. D.K. Hoover, E.W.L. Chan and M.N. Yousaf*. J. Am. Chem. Soc. 2008, 130, 3280-3281.
Highlighted in Faculty of 1000.
Live-Cell Fluorescence Microscopy of Directed Cell Migration on Partially Etched Electroactive SAM Gold Surfaces. B.M. Lamb, N.P. Westcott, M.N. Yousaf*. ChemBioChem 2008, 9, 2220-4.
Microfluidic Etching and Oxime-Based Tailoring of Biodegradable Polyketoesters. D.G. Barrett, B.M. Lamb, M. N. Yousaf*. Langmuir 2008, 24, 9861-9867.
Microfluidic Lithography of SAMs on Gold to Create Dynamic Surfacesfor Directed Cell Migration and Contiguous Cell Co-Cultures. B.M. Lamb, D.G. Barrett, N.P. Westcott, M .N. Yousaf*. Langmuir 2008, 24, 8885-9.
Preparation of a Class of Versatile, Chemoselective, and Amorphous Polyketoesters D. G. Barrett and M. N. Yousaf*. Biomacromolecules 2008, 9, 2029-2035.
An Interfacial Oxime Reaction to Immobilize Ligands and Cells in Patterns and Gradients to Photo-Active Surfaces. S. Park, M.N. Yousaf*. Langmuir 2008, 24, 6201-6207.
A Photo-Electroactive Surface Strategy for Immobilizing Ligands in Patterns and Gradients for Studies of Cell Polarization. E.W.L. Chan and M.N. Yousaf*. Mol. BioSys. 2008, 4, 746-753.
Synergistic Microfluidic and Electrochemical Strategy to Activate and Pattern Surfaces Selectively with Ligands and Cells. N.P. Westcott and M.N. Yousaf*. Langmuir 2008, 24, 2261-2265.
A Tunable, Chemoselective and Moldable Biodegradable Polyester for Cell Scaffolds. D.G. Barrett and M.N. Yousaf*. ChemBioChem. 2008, 9, 62-66.
Combining Surface Chemistry with a FRET-Based Biosensor to Study the Dynamics of RhoA GTPase Activation in Cells on Patterned Substrates. L.Hodgson, E.W.L. Chan, K.M. Hahn and M.N. Yousaf*. J. Am. Chem. Soc. 2007, 129, 9264-9265.
Rapid Patterning of Cells and Cell Co-Cultures with Spatial and Temporal Control on Surfaces through Centrifugation. D.G. Barrett and M.N. Yousaf*. Angew. Chem. Int. Ed. 2007, 46, 7437-7439.
Surface-Chemistry Control to Silence Gene Expression in Drosophila Schneider 2 Cells through RNA Interference. E.W.L. Chan and M.N. Yousaf*. Angew. Chem. Int. Ed. 2007, 46, 3881-3884.
Electroactive nanoarrays for Biospecific Ligand Mediated Cell Adhesion. D.K. Hoover, E.J. Lee, E.WL. Chan and M.N. Yousaf*. ChemBioChem 2007, 8, 1920-1923.
Site-Selective Immobilization of Ligands with Control of Density on Electroactive Microelectrode Arrays. E.W.L. Chan and M.N. Yousaf*. ChemPhysChem 2007, 8, 1469-1472.
Selective Immobilization of Ligands, Proteins and Cells to Electroactive Surfaces. E.W.L. Chan and M.N. Yousaf*. J. Am. Chem. Soc. 2006, 128, 15542-15546.
Highlighted in Faculty of 1000. Nanowerks.
A Photochemical Method for Patterning the Immobilization of Ligands and Cells to Self-Assembled Monolayers W. S. Dillmore, M.N. Yousaf and M. Mrksich Langmuir 2004, 20, 7223-7231.
Dynamic Interfaces Between Cells and Surfaces: Electroactive Substrates that Sequentially Release and Attach Cells. W.S. Yeo, M.N. Yousaf, M. Mrksich. J. Am. Chem. Soc., 2003, 125,14994-14995.
Featured in: News of the Week, Chemical Engineering News 2003, 81, 11
Using Electroactive Substrates to Pattern the Attachment of Two Different Cell Types. M.N. Yousaf, B.T. Houseman and M. Mrksich Proc. Natl. Acad. Sci. (USA) 2001, 98, 5992-5996.
Turning on Cell Growth with Electroactive Substrates. M.N. Yousaf, B.T. Houseman and M. Mrksich Angew. Chem. Int. Ed. 2001, 40, 1093.
Featured in: News of the Week, Science 2000, 1477
Nature News Wire March 27, 2001.
News of the Week, Chemical and Engineering News 2001, 79, 13
Understanding the Role of Adsorption in the Reaction of Cyclopentadiene with an Immobilized Dienophile. E.W.L. Chan, M.N. Yousaf and M. Mrksich J. Phys. Chem. A. 2000, 104, 9315.
The Kinetic Order of an Interfacial Diels-Alder Reaction Depends on the Environment of the Immobilized Dienophile. M.N. Yousaf, E.W.L. Chan and M. Mrksich Angew. Chem. Int. Ed. 2000, 39, 1943.
Diels-Alder Reacton for the Selective Immobilization of Protein to Electroactive Substrates. M.N. Yousaf and M. Mrksich J. Am. Chem. Soc. 1999, 121, 4286.
Featured in: Science Concentrates, Chemical and Engineering News 1999, 77, 11
Modulating the Behaviors of Attached Cells with Dynamic Substrates. M.N. Yousaf and M. Mrksich New Technologies for Life Sciences. A Trends Guide. 2000, 28-35.
Copper (II) complexes of bis(1,4,7-triazacyclononane) ligands with polymethylene bridging groups: An equilibrium and structural study. R. Haidar, M. Ipek, M.N. Yousaf and L.J. Zompa. Inorg. Chem., 1997, 36, 3125.
Patent: World Intellectual Property Patent (International Application #: PCT/US2004/009376) regarding the Determination of Proteins and/or other Molecules Using MALDI Mass Spectroscopy.