Day 1: Understanding EV Therapeutics: From Basics to Translation
10 am Registration (30')
10:30 am-11:15 Why EV therapy?
Ahmed Ibrahim, PhD, Cedars Sinai Medical Center, USA
11:15-12 pm Native EVs for therapy
Darryl Davis, MD, Ottawa University, Ca
12-12:45 pm Engineered EVs for therapy
Dave Carter, PhD, Evox Therapeutics Ltd, Oxford-UK
12:45-1:45 pm COFFEE + lunch break with faculty
1:45-2:30 pm Synthetic drug carriers
Evan Scott, PhD, University of Virginia, USA
2:30-3:15 pm Extracellular Vesicles in Modern Medicine: Bridging the lab and the Clinic
Susmita Sahoo, PhD, Icahn School of Medicine at Mount Sinai, USA
3:15-4 pm Inhalable EVs for lung disease therapy
Mengrui Liu, PhD, Columbia University
4-4:45 pm Translation to clinic
Mario Gimona, PhD, aracelsus Medical Private University (PMU) in Salzburg Austria
4:45 pm – end Ice breaker-network for students – meet and greet
Day 2: Introduction to the EV field and EV enrichment methods
9-9:15 am Outline on the day
Uta Erdbrügger – University of Virginia
9:15-10 am Lecture: Introduction to EV heterogeneity
Edit I. Buzás – Semmelweis University, Budapest, Hungary (virtual)
10-10:15 am Coffee break
10:15-11 am Lecture: Introduction to ISEV workforces and MISEV initiative
Joshua A. Welsh – BD Biosciences
11-11:45 am Lecture: EV separation methods: an overview
Luca Musante - University of Pennsylvania, USA
11:45- noon Short talks
Industry representative Everest Biolabs
12-1 pm Lunch break with faculty
1-3:30 pm – Hands-on EV separation
Luca Musante - University of Pennsylvania
Sabrina LaSalvia, Icahn School of Medicine at Mount Sinai, USA
Industry representative Everest Biolabs
3:30-5pm – Coffee and Tailored feedback session 1
Participant poster presentations (group 1)
5pm: Rotunda visit Networking and Group Bonding: Building Connections Together
Day 3: Basic EV characterization 1- counting, sizing, and imaging
9-9:15 am Outline on the day
Uta Erdbrügger – University of Virginia
9:15-10 am Lecture: Introduction to Electron Microscopy Imaging
Guillaume Van-Niel – Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers, France (virtual)
10-10:15 am Coffee break
10:15-11 am Lecture: Comprehensive Insights into EV Instrumentation
Shannon L. Stott – Harvard University, USA
11-11:30 am Short talks
Industry representatives: Particle Metrix and ONI
11:30-1 pm Lunch break with faculty
1-4 pm – Hands-on EV counting, sizing, and imaging
Industry representatives: Particle Metrix, Izon, and ONI
4.30-6 pm – Coffee and Tailored feedback session 2
Participant poster presentations (group 2)
Day 4: Basic EV characterization 2- Omics
9-9:15 am Outline on the day
Uta Erdbrügger – University of Virginia
9:15-10 am EV microarrays and EV nanoscope to map the EV universe from cell media to patient samples
David Juncker – McGill University
10-10:15 am Coffee break
10:15- 11 Lecture: EV Transcriptomics
Navneet Dogra – Icahn School of Medicine at Mount Sinai, USA
11:15 am-12:30 pm Participant poster presentations (group 3)
12:30-1:30 pm – Lunch break with faculty
1:30-2.15 pm – Lecture Proteomics (pre-recorded because of time zone)
David W. Greening - Baker Heart and Diabetes Institute
2.15 – 2.45 pm Q & A zoom live discussion
David W. Greening - Baker Heart and Diabetes Institute
3-3:05 pm Flow training introduction
Michael D. Solga – Virginia University
3:05-3:35 pm Lecture: Instrument Optimization/Calibration
Joshua A. Welsh – BD Biosciences
3:35-4:05 pm Lecture: Quality Control/Quality Assurance
Joanne Lannigan – Flow Cytometry Support Services, LLC
4:05–4:20 pm: Industry short talk
Industry representatives-Beckman
6.30 pm Networking Dinner in town: Kardinal Hall
Day 5: EV Flow Cytometry- Instrument Optimization, Calibration, and Data Acquisition
8-10:45 am EV Flow Hands-on: Group 1
Joanne Lannigan – Flow Cytometry Support Services, LLC, Michael D. Solga – Virginia University, & Joshua A. Welsh – BD Biosciences
Bead-based EV detection with Miltenyi: Group 2
Group 3
10:45 am coffee break and moving to next session
11 am 1:45 pm EV Flow Hands-on: Group 2
Joanne Lannigan – Flow Cytometry Support Services, LLC, Michael D. Solga – Virginia University, & Joshua A. Welsh – BD Biosciences
Bead-based EV detection with Miltenyi: Group 3
Group 1
1:45-2:30 pm – Lunch break on your own/ Coffee provided
2:30-5:15 pm
EV Flow Hands-on: Group 3
Joanne Lannigan – Flow Cytometry Support Services, LLC, Michael D. Solga – Virginia University, & Joshua A. Welsh – BD Biosciences
Bead-based EV detection with Miltenyi: Group 1
Group 2
5:15- 5:30 pm Drinks and flow data- Josh and Joanne
5:30- end Short Summarizing the do's and don'ts and closing remarks