Day 1
4:00–4:30 pm: Registration
4:30–4:45 pm: Introduction to SNEV – Natalie Turner (The Scripps Research Institute)
4:45–5:30 pm: Icebreaker & Participant Presentations
5:30–6:30 pm: Networking & Light Bites
Day 2
9:00–9:15 am: Day Outline – Olesia Gololobova (Johns Hopkins University)
9:15–10:00 am: Keynote I: Ahmad Ibrahim (Cedars Sinai) – Between Investigation and Translation
10:00–10:15 am: Coffee Break
10:15–11:45 am: Lectures:
Joshua A. Welsh (BD Biosciences): ISEV Workforces & MISEV Initiative
Edit I. Buzás (Virtual, Semmelweis University): EV Heterogeneity
11:45 am–12:30 pm: Luca Musante (Pennsylvania University): EV Isolation Methods
12:30–1:30 pm: Lunch Break
1:30–3:30 pm: Hands-on EV Isolation – Luca Musante & Olesia Gololobova
3:30–5:00 pm: Coffee & Tailored Feedback – Poster Presentations (Group 1)
Day 3
8:30–8:35 am: Day Outline – Uta Erdbrügger (Virginia University)
8:35–9:15 am: Keynote II: Evan Scott (Virginia University) – Nanoparticle Targeting Strategies
9:15–10:00 am: Shannon L. Stott (Harvard University): EV Instrumentation
10:00–10:30 am: Short Industry Talks – Particle Metrix & Izon
10:30–12:30 pm: Coffee, Lunch, & Poster Presentations (Group 2)
12:30–2:30 pm: Hands-on Counting & Sizing – Particle Metrix & Izon
2:30 pm: Networking & Group Bonding – Monticello Tour
Day 4
9:00–9:15 am: Day Outline – Olesia Gololobova
9:15–10:00 am: Guillaume Van-Niel (Virtual): Introduction to Electron Microscopy
10:00–10:30 am: Coffee Break
10:30–11:15 am: Faculty 1:1 Sessions – Uta, Olesia, Leyla, Shannon, Josh, Joanne
11:15 am–12:00 pm: Leyla Esfandiari (University of Cincinnati): Bulk vs. Single EV Characterization
12:00–1:00 pm: Lunch Break
1:00–1:45 pm: Industry Insights – Exoview, ONI, Beckman
1:45–3:45 pm: Hands-on Single Particle Imaging – ONI & Exoview
4:30–6:00 pm: Optional Tour – UVA Molecular Electron Microscopy Core Facility (Cryo-EM) – Hayrettin Yavuz, Michael Purdy and David Cooper
6:30 pm: Networking Dinner at Kardinal Hall
Day 5
8:00–8:05 am: Day Outline – Michael D. Solga (Virginia University)
8:05–9:05 am: Lectures:
Joshua A. Welsh: Instrument Optimization & Calibration
Joanne Lannigan: Quality Control/Assurance
9:05–9:35 am: Coffee Break
9:35 am–12:45 pm: Hands-on Flow Cytometry – Group 1
12:45–1:45 pm: Lunch Break (on your own) – Coffee Provided
1:45–4:15 pm: Hands-on Flow Cytometry – Group 2
Day 6
8:45–9:00 am: Day Outline – Uta Erdbrügger
9:00–10:00 am: EV Flow Data Analysis – Joanne Lannigan, Michael D. Solga, Joshua A. Welsh
10:00–10:30 am: Coffee Break
10:30–11:15 am: Summary of Best Practices & Closing Remarks – Uta Erdbrügger, Tanina Arab, Olesia Gololobova
11:15 am–12:30 pm: Navneet Dogra (Mount Sinai): Transcriptomics
12:30–1:30 pm: Lunch Break
1:30–2:15 pm: David W. Greening (Baker Institute): Proteomics
2:15–2:45 pm: Q&A: Omics – David W. Greening & Navneet Dogra
2:45 pm: Farewell