Day-wise Schedule

4:00–4:30 pm: Registration

4:30–4:45 pm: Introduction to SNEV – Natalie Turner (The Scripps Research Institute)

4:45–5:30 pm: Icebreaker & Participant Presentations

5:30–6:30 pm: Networking & Light Bites

9:00–9:15 am: Day Outline – Olesia Gololobova (Johns Hopkins University)

9:15–10:00 am: Keynote I: Ahmad Ibrahim (Cedars Sinai) – Between Investigation and Translation

10:00–10:15 am: Coffee Break

10:15–11:45 am: Lectures:

Joshua A. Welsh (BD Biosciences): ISEV Workforces & MISEV Initiative

Edit I. Buzás (Virtual, Semmelweis University): EV Heterogeneity

11:45 am–12:30 pm: Luca Musante (Pennsylvania University): EV Isolation Methods

12:30–1:30 pm: Lunch Break

1:30–3:30 pm: Hands-on EV Isolation – Luca Musante & Olesia Gololobova

3:30–5:00 pm: Coffee & Tailored Feedback – Poster Presentations (Group 1)

8:30–8:35 am: Day Outline – Uta Erdbrügger (Virginia University)

8:35–9:15 am: Keynote II: Evan Scott (Virginia University) – Nanoparticle Targeting Strategies

9:15–10:00 am: Shannon L. Stott (Harvard University): EV Instrumentation

10:00–10:30 am: Short Industry Talks – Particle Metrix & Izon

10:30–12:30 pm: Coffee, Lunch, & Poster Presentations (Group 2)

12:30–2:30 pm: Hands-on Counting & Sizing – Particle Metrix & Izon

2:30 pm: Networking & Group Bonding – Monticello Tour

9:00–9:15 am: Day Outline – Olesia Gololobova

9:15–10:00 am: Guillaume Van-Niel (Virtual): Introduction to Electron Microscopy

10:00–10:30 am: Coffee Break

10:30–11:15 am: Faculty 1:1 Sessions – Uta, Olesia, Leyla, Shannon, Josh, Joanne

11:15 am–12:00 pm: Leyla Esfandiari (University of Cincinnati): Bulk vs. Single EV Characterization

12:00–1:00 pm: Lunch Break

1:00–1:45 pm: Industry Insights – Exoview, ONI, Beckman

1:45–3:45 pm: Hands-on Single Particle Imaging – ONI & Exoview

4:30–6:00 pm: Optional Tour – UVA Molecular Electron Microscopy Core Facility (Cryo-EM) – Hayrettin Yavuz, Michael Purdy and David Cooper

6:30 pm: Networking Dinner at Kardinal Hall

8:00–8:05 am: Day Outline – Michael D. Solga (Virginia University)

8:05–9:05 am: Lectures:

Joshua A. Welsh: Instrument Optimization & Calibration

Joanne Lannigan: Quality Control/Assurance

9:05–9:35 am: Coffee Break

9:35 am–12:45 pm: Hands-on Flow Cytometry – Group 1

12:45–1:45 pm: Lunch Break (on your own) – Coffee Provided

1:45–4:15 pm: Hands-on Flow Cytometry – Group 2

8:45–9:00 am: Day Outline – Uta Erdbrügger

9:00–10:00 am: EV Flow Data Analysis – Joanne Lannigan, Michael D. Solga, Joshua A. Welsh

10:00–10:30 am: Coffee Break

10:30–11:15 am: Summary of Best Practices & Closing Remarks – Uta Erdbrügger, Tanina Arab, Olesia Gololobova

11:15 am–12:30 pm: Navneet Dogra (Mount Sinai): Transcriptomics

12:30–1:30 pm: Lunch Break

1:30–2:15 pm: David W. Greening (Baker Institute): Proteomics

2:15–2:45 pm: Q&A: Omics – David W. Greening & Navneet Dogra

2:45 pm: Farewell