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Description
Description
The Nanoliter Osmometer is a high-precision temperature-controlled stage designed for optical microscopy. It allows for precise control of the sample temperature (<0.001°C), enabling detailed observation of ice nucleation, growth, and melting.
The Nanoliter Osmometer is controlled by special software that enables various types of temperature control, such as step, ramp and dwell. The software also allows synchronized video recording to directly monitor and analyze changes in the physical characteristics of the sample, such as size, shape, and optical signal.
Application
Application
The Nanoliter Osmometer was originally designed to investigate micron-sized ice crystals behavior under an optical microscope. It allows the determination of an aqueous solution's melting and freezing point using nanoliter droplets. Such measurements are extremely valuable for quantitative evaluation of antifreeze activity, re-crystallization studies, and more.
Documentation
Documentation
Example studies using a Nanoliter Osmometer
Example studies using a Nanoliter Osmometer
Hansen, T., Lee, J. C., Reicher, N., Ovadia, G., Guo, S., Guo, W., ... & Davies, P. L. (2023). Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0° C, eLife 12:RP91976.
Lee, J. C., Hansen, T., & Davies, P. L. (2023). Droplet freezing assays using a nanoliter osmometer. Cryobiology, 113, 104584.
Scholl, C. L., Holmstrup, M., Graham, L. A., & Davies, P. L. Polyproline type II helical antifreeze proteins are widespread in Collembola and likely originated over 400 million years ago in the Ordovician Period. Scientific Reports. 2023, 13(1), 8880.
Braslavsky I, Drori R. LabVIEW-operated novel nanoliter osmometer for ice binding protein investigations. J Vis Exp. 2013 Feb 4;(72):e4189. doi: 10.3791/4189.
Berger T, Meister K, DeVries AL, Eves R, Davies PL, Drori R. Synergy between Antifreeze Proteins Is Driven by Complementary Ice-Binding. J Am Chem Soc. 2019 Dec 4;141(48):19144-19150. doi: 10.1021/jacs.9b10905.
Mizrahy O, Bar-Dolev M, Guy S, Braslavsky I. Inhibition of ice growth and recrystallization by zirconium acetate and zirconium acetate hydroxide. PLoS One 2013; 8(3):e59540. doi: 10.1371/journal.pone.0059540.
Bar-Dolev M, Celik Y, Wettlaufer JS, Davies PL, Braslavsky I. New insights into ice growth and melting modifications by antifreeze proteins. J R Soc Interface. 2012 Dec 7;9(77):3249-59. doi: 10.1098/rsif.2012.0388.
Specifications
Specifications
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