At KBRI
Hwang J#, Park K#, Choi JS, Riew TR, Cho KO, No KT, Lim HH*. Cryo-EM structures of an anti-MLC1 Fab in apo and peptide-bound states reveal the structural basis of antigen recognition. bioRxiv 2025.10.08.681078.
Hwang J, Kim E, Kim J, Shin S, and Lim HH*. Integrated Isotyping and CDR Identification of Mouse Monoclonal Antibodies Using Multiplex RT-PCR. Methods, 244: Pages 134-142, 2025. (part of special issue, Editors Collection-YMETH)
Kim D, Koh Y, Jeong HS, Kim J, Do H, Son G, Kim Y, Kim D, Lim HH*, Park JE*, Han J*. MEG3 Enhances Survival of Developing Human Neurons with CLCN4-Linked Autophagy Impairment. bioRxiv 2025.07.16.665078. (*Co-corresponding Author)
Park K, Yoo Y, Jeon H, Choi K, Kwon E, Lim HH, Kim DY, No KT. High-resolution cryo-EM structures of small protein–ligand complexes near the theoretical size limit. bioRxiv 2025.06.30.662489.
Kim KW#, Lee EA#, Ko A#, Hwang J, Park K, Lee BC, Kim KW, Oh WJ, Kim K, and Lim HH*. Cryo-EM structures of mouse bestrophin 1 channel in closed and partially open conformations. Mol. Cells, 100208, 2025.
Ko W, Lee E, Kim JE, Lim HH, Suh BC. The plasma membrane inner leaflet PI(4,5)P2 is essential for the activation of proton-activated chloride channels. Nat. Comm. 15:7008, 2024.
Kim N, Li Y, Yu R, Kwon HS, Song A, Jun MH, Jeong JY, Lee JH, Lim HH, Kim MJ, Kim JW, Oh WJ. Repulsive Sema3E-Plexin-D1 signaling coordinates both axonal extension and steering via activating an autoregulatory factor, Mtss1. eLife 13:e96891. 2024.
Lee Y, Jeong HS, .. , Hwang J, .. , Lim HH, and Lee S. Cryo-EM structures of the plant anion channel SLAC1 from Arabidopsis thaliana suggest a combined activation model. Nat. Comm. 14:7345, 2023.
Kim SK, ...., Hwang J, Lim HH, .., Kim KW. A mitochondria-derived peptide, SHLP2, regulates energy homeostasis through the activation of hypothalamic POMC neurons. Nat. Comm. 14:4321, 2023.
Yoo H, … Park K, Lim HH*, Son CY*, Sun JY* and Oh SS*. G-quadruplex-filtered selective ion-to-ion current amplification for non-invasive ion monitoring in real time. Advanced Materials e2303655, 2023. (*Co-corresponding Author)
Kim K#, Hwang J#, Kim DH, Park HJ, and Lim HH*. Cytosolic domain regulates the calcium sensitivity and surface expression of BEST1 channels in the HEK293 cells. BMB Reports 56: 172-177, 2023.
Hwang J, Park K, Lee G, Yoon BY, Kim H, Roh SH, Lee BC, Kim K, and Lim HH*. Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1. Open Biology 11: 210103, 2021.
Mersch K, Ozturk TN, Park K, Lim HH, and Robertson JL. Altering CLC stoichiometry by reducing non-polar side-chains at the dimerization interface. J. Mol. Biol. 433(8): 166886, 2021.
Lee HJ, Jeong H, Hyun J, Ryu B, Park K, Lim HH, Yoo J, and Woo JS. Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel. Science Advances 6: eaba4996, 2020.
Hwang J, Vu HM, Kim MS, and Lim HH*. Plasma membrane localization of MLC1 regulates cellular morphology and motility. Molecular Brain 12: 116 doi:10.1186/s13041-019-0540-6, 2019.
Park K, Lee BC, and Lim HH*. Mutation of external glutamate residue reveals a new intermediate transport state and anion binding site in a CLC Cl-/H+ antiporter. Proc. Natl. Acad. Sci. USA. 116: 17345-17354, 2019.
Lim HH*, Kim KK, and Park CS. Introduction to the Korean Biophysical Society (KBPS). Biophysical Review. 11(3). doi: 10.1007/s12551-019-00547-w. 2019. (Corresponding author)
Yeon J, Kim J, Kim DY, Kim H, Kim J, Du EJ, Kang K, Lim HH, Moon D, and Kim K. A sensory-motor neuron type mediates proprioceptive coordination of steering in C. elegans via two TRPC channels. PLoS Biology 16(6): e2004929, 2018.
Seong JY, Ha K, Hong C, Myeong J, Lim HH, Yang D, and So I. Helix O modulates voltage dependency of CLC-1. Pflugers Arch. 469(2):183-193. 2017.
Jeong SJ, Lee H, Hur EM, Choe Y, Koo JW, Rah JC, Lee KJ, Lim HH, Sun W, Moon C, Kim K. Korea Brain Initiative: Integration and Control of Brain Functions. Neuron. 92(3):607-611. 2016.
Graduate / Post-doctoral Research
Lim HH, Stockbridge RB, and Miller C. Fluoride-dependent interruption of the transport cycle of a CLC Cl–/H+ antiporter. Nat. Chem. Biol. 9(11):721-725, 2013.
Lim HH, Shane T and Miller C. Intracellular proton access in a Cl-/H+ antiporter. PLoS Biology, 10(12): e1001441, 2012. (Recommended by Faculty of 1000)
Phillips S, Brammer A, Rodriguez L, Lim HH, Stary-Weinzinger A, and Matulef K. Surprises from unusual CLC homolog. Biophys. J. 103: L44-L46, 2012.
Stockbridge RB, Lim HH, Otten R, Williams C, Shane T, Weinberg Z, and Miller C. Fluoride resistance and transport by riboswitch-controlled CLC antiporters. Proc. Natl. Acad. Sci. USA, 109:15289-15294, 2012.
Lim HH and Miller C. It takes two to transport, or is it one? Nat. Struct. Mol. Biol. 19:129-130, 2012. (Corresponding author)
Lim HH*#, Fang Y#, Williams C. High-efficiency screening of monoclonal antibodies for membrane protein crystallography. PLoS ONE 6: e24653, 2011. (Corresponding author)
Lim HH and Miller C. Intracellular proton-transfer mutants in a CLC Cl-/H+ exchanger. J. Gen. Physiol. 133:131-138, 2009. (of interest article in J. Cell Biol. 184, 2009)
Lee BC, Lim HH, Kim S, Youn HS, Lee Y, Kim YC, Eom SH, and Park CS. Localization of the site of action for benzofuroindole-induced potentiation of BKCa channels. Mol. Pharmacol. 82:143-155, 2012.
Lee BC, Hong SE, Lim HH, Kim DH, Park CS. Alteration of the transcriptional profile of human embryonic kidney cells by transient overexpression of mouse TRPM7 channels. Cell. Physiol. Biochem. 27: 313-326, 2011.
Lee JH, Kang GB, Lim HH, Jin KS, Kim SH, Ree M, Park CS, Kim SJ, Eom SH. Crystal structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with L-glutamate: structural dissection of the ligand interaction and subunit interface. J. Mol. Biol. 376:308-316. 2008.
Kim HJ, Lim HH, Rho SH, Bao L, Lee JH, Cox DH, Kim do H, Park CS. Modulation of the conductance-voltage relationship of the BKCa channel by mutations at the putative flexible interface between two RCK domains. Biophys. J. 94:446-456. 2008.
Jung KY, Moon HD, Lee GE, Lim HH, Park CS, Kim YC. Structure-activity relationship studies of spinorphin as a potent and selective human P2X3 receptor antagonist. J. Med. Chem. 50:4543-4547. 2007.
Kim HJ, Lim HH, Rho SH, Eom SH, Park CS. Hydrophobic interface between two regulators of K+ conductance domains critical for calcium-dependent activation of large conductance Ca2+-activated K+ channels. J. Biol. Chem. 281:38573-38581. 2007.
Ha TS, Lim HH, Lee GE, Kim YC, and Park CS. Electrophysiological characterization of benzofuroindole-induced potentiation of large-conductance Ca2+-activated K+ channel. Mol. Pharmacol. 69:1007-1014, 2006.
Kim BJ, Lim HH, Yang DK, Jun JY, Chang IY, Park CS, So I, Stanfield PR and Kim KW. Melastatin type transient receptor potential channel 7 is required for intestinal pacemaking activity. Gastroenterology 129:1504-1517, 2005.
Lim HH and Park CS. Identification and functional characterization of ankyrin-repeat family protein ANKRA as a protein interacting with BKCa channel. Mol. Biol. Cell 16:1013-1025, 2005.
Lim HH, Park BJ, Choi HS, Park CS, Eom SH, and Ahnn J. Identification and characterization of a putative C.elegans potassium channel gene (Ce-slo-2) distantly related to Ca2+-activated K+ channels. Gene 240:35-43, 1999.
Manuscript in preparation
Park K#, Kim J#, ... , Roh SH*, and Lim HH*. Cryo-EM structure of a voltage- and pH-dependent CLC Cl-/H+ antiporter (In preparation)
Lee BC#, Park K#, Hwang J, Jeong HS, Kim J, So I, and Lim HH*. Coordinated gating of the inner and outer gates in a CLC-type Cl-/H+ antiporter. (In preparation)
Hwang J#, Lee E#, Kim K, Park K, Vu HM, Kim M, and Lim HH*. Cryo-EM structure and function of the hBEST1 channel and metabolic enzyme complex. (in preparation)
Kim KW#, Jeong H#, Park K, Lee E, and Lim HH*. Structural flexibility and the anion permeation mechanism of the human BEST1 channel. (In preparation)
Lim HH et al., Monoclonal antibody specifically binding to MLC1 protein and use thereof, 2025. (KR patent)
Lim HH et al., Method for effective purification of human TREM2 proteins using recombinant baculovirus, 2020. (KR patent)
Lim HH et al., Monoclonal antibody with specificity for human TREM2 protein, hybridoma cell line producing the same and use thereof, 2020. (KR patent)