We have edited our research paper and have submitted it to be considered for publication in the Journal of Emerging Investigators! As of September 1st, 2025, our paper has been submitted, and we are waiting to hear more about it being published.
To conclude our year, we presented our project at the Rock Canyon High School Research Symposium! We enjoyed sharing our findings with the RCHS community. Currently, we are wrapping up our paper to be published in a scientific journal very soon. Thanks for following along this year with our research!
After completing our research and analyzing our data, we were able to determine that there was a statistically significant decrease in the growth of the experimental flasks, meaning Teleglenastat was an effective drug in reducing the growth of the EO771 cells. We presented our research at the Colorado-Wyoming Junior Academy of Science science fair on April 12th, 2025. We were honored to receive a third place overall as well as a first place finish in our session, as well as qualifying for the American Junior Academy of Science Annual Conference in February of 2026.
After a month of taking thousands of pictures of cells with a hemocytometer and counting cells, we have concluded trials! We are currently analyzing the data to determine our results and finish our presentation and paper.
We started our first experimental trial! We worked hard all day, using the hemocytometer and taking hundreds of pictures of our cells. This was the first part of our first trial, collecting data for the cells prior to being exposed to 1 micromolar teleglenastat solution for 72 hours. On Monday, we collected the final data for the cells and will begin counting the cells to determine our results.
We have continued with culturing our EO771 cells in adhesion culture flasks and have got them to grow enough that we have been able to move some into suspension flasks. The suspension flasks are not culture treated so the cells grow suspended in the media instead of growing attached to the flask. This type of culture is significant to our research because it models the cancer cells when they have spread into the bloodstream beyond just breast tissue.
Brandon and Lila took another trip up to Anschutz to pick up more frozen vials of EO771 cells from our amazing mentor, Mr. Williams. We had some issues with growing the cells due to them not adhering to our adherent flasks but we did some troubleshooting and have made some small changes to our protocol. We are excited to continue culturing these new vials of cells.
We are hard at work growing our cells! We have been working on refining our protocols for splitting and changing the media for the cells to promote growth. We hope to begin experimental trials soon and begin collecting data!
Happy new year! We are back and are working hard on our trials for the cells. We have plated another vial of cells and are beginning to see growth. We have began growing our cells in T25 adherent flasks to speed up the growth process. Once the cells become confluent, we will move them into T75 adherent flasks and also our T75 non-culture treated flasks for our suspension cultures. We are excited to get going on our research!
Due to our winter break, we had to aspirate our cells for the break. Prior to this, we had plated one vial of our EO771 cells and practiced culturing them. We were able to see cell growth and practice procedures for changing our media and splitting our cells. We are so excited to get back to our research after the break. Happy holidays!!
Lila and Kayleigh went to CU Anschutz to pick up our EO771 cells from our amazing mentor, Jared. We now will begin our pre-trials and become familiar with our methods of cell culture.
We cleaned the cell culture room to make sure it was sterile and ready for us to have our cells in it. We are so excited to begin our research! Make sure to stay tuned for more updates as we plan to receive our cells and begin pre-trials soon.
We pitched our research to a panel of doctors and researchers and answered questions about our prospective research. We got approved and are so excited to begin our research! Thanks so much to everybody who came either virtually or in-person to support us, we appreciate you all!!Â
We went on a class field trip to the CU Anschutz medical campus and got to meet our out of class mentor, Jared Williams. We got a tour of his lab and got to see what he has been researching. We also learned about the bioengineering field and saw other labs at Anschutz. Overall, it was a great trip and an amazing experience!