大阪大学 大学院 工学研究科 附属フューチャーイノベーションセンター
テクノアリーナ准教授 /若手卓越教員
大阪大学 大学院 工学研究科 物理学系専攻 応用物理学コース
助教（兼任）
TechnoArea Associate Professor
Graduate School of Engineering, Future innovation center, Osaka University
Assistant Professor
Graduate School of Engineering, Department of Applied Physics Osaka University
Motivation:
私はレーザー光による複雑系ソフトマターの計測・制御を進めてきました.対象とするソフトマターは，タンパク質分子結晶から臓器までの幅広い「動く，生きたソフトマター」であり，そのしなやかさを生む「物性と構造」の定量解明を目指しています．
2013年に埼玉大学 理学部 基礎化学科（中林誠一郎教授・吉川洋史教授）でJSPS-DC1を実施し，2016年に博士取得後は，JSPS-PDで横浜市立大学 医学部（谷口英樹教授）および東京医科歯科大学 統合研究機構（武部貴則教授）で臓器再生の多様な課題を計測・解析してきました（CellStemCell 2015 雑誌表紙, Stem Cell Reports 2018）．2019年に古巣の埼玉大学助教に着任してからは（中林誠一郎教授），2021年のJST創発的研究支援事業において，田中純子パネルの一期生として採択されました．同年の夏には2021年には大阪大学 応用物理 助教（吉川洋史教授）として異動しました．
2022年4月には大阪大学大学院工学研究科附属フューチャーイノベーションセンター「若手卓越教員」として称号を獲得し，若手PIになりました．特に，物理化学者PIの責任著者*として，顕微鏡開発を進める傍ら（J.Phys.Chem.Lett. 2022(Supplimentary Cover)* & 2024, Bioconj. Chem. 2023 Cover*, 化学と生物 2023Cover*）, PNAS 2024），医学部との共同研究も盛んに進めながら，肝臓や筋肉（武部貴則教授：iScience 2022* StarProtocol 2023*），大腸（竹田潔教授：Mucosal Immunology 2023, Method Mol Biol. 2024*），軟骨（関矢一郎教授：Stem Cell Research and Therapy 2022,iScience 2024）と多臓器に展開中です．一人の阪大の工学者として，多様な医学・生物学の先生と連携しながら，臓器における硬さの役割解明し続けています．
以上より、阪大を中心とした更なる医理工連携の拡大と，硬さに基づく新たな臓器再生の分野創生（臓器再生のメカノバイオロジー）に向けて，多くの先生方とのコラボレーションをお待ちしております．
I have been working on the measurement and control of complex soft matter using laser beams. The target soft matter is a wide range of "moving, living soft matter" from protein molecular crystals to organs, and I aim to quantitatively elucidate the "physical properties and structures" that give rise to their suppleness.
In April 2022, I was awarded the title of "Young Outstanding Researcher" of the Future Innovation Centre, Osaka University, Graduate School of Engineering, and became a young PI. In particular, as a responsible author* of the Physical Chemist PI, while promoting microscopy development (J. Phys. Chem. Lett. 2022(Supplementary Cover)* & 2024, Bioconj. Chem. 2023 Cover*, Chemistry and Biology 2023Cover* PNAS 2024.), he has been actively conducting collaborative research with the Faculty of Medicine. While actively pursuing research, we are also conducting joint research with the Faculty of Medicine on liver and muscle (Prof Takanori Takebe: iScience 2022* StarProtocol 2023*), colon (Prof Kiyoshi Takeda: Mucosal Immunology 2023, Method Mol Biol. 2024*), cartilage (Prof Ichiro Sekiya: Stem Cell Research and Therapy 2022, iScience 2022* StarProtocol 2023*) and cartilage (Prof Ichiro Sekiya: Stem Cell Research and Therapy 2022, iScience 2023* StarProtocol 2023*). Research and Therapy 2022, iScience 2024), and cartilage (Prof. Ichiro Sekiya: Stem Cell Research and Therapy 2022, iScience 2024). As an engineer at Osaka University, I continue to elucidate the role of stiffness in organs in collaboration with various medical and biological professors.
We are therefore looking forward to collaborating with many professors to further expand medical-engineering collaboration centred on Osaka University and to create a new field of organ regeneration based on stiffness (mechanobiology of organ regeneration).
CV
2022-current
TechnoArenaAssociateProfessor,CenterforFutureInnovation,GraduateSchoolofEngneering, Osaka University, Japan (PI)
2022 -current
TechnoArena Excellent Young Researcher, Center for Future Innovation, Graduate School of Engneering, Osaka University, Japan (PI)
2021 -current
Assistant professor, Department of Applied Physics, Graduate School of Engneering, Osaka University, Japan (Prof. Hiroshi Yoshikawa Lab)
2019-2021
Assistant Professor, Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Japan (Prof. Seiichiro Nakabayashi, Prof. Hiroshi Yoshikawa Lab)
2018-2019
Japan Society for the Promotion of Science (JSPS) Research Fellow (Postdoctor), Institute of Research, Tokyo Medical and Dental University, Japan (Concurrent)
2016-2019
Japan Society for the Promotion of Science (JSPS) Research Fellow (Postdoctor), Department of Regenerative Medicine, Yokohama City University, Japan (Prof. Takanori Takebe Lab)
2015-2016
Visiting students, Biophysical Chemistry Laboratory, Institute of Physical ChemistryInstitute of Physical Chemistry, Heidelberg University, Germany (Prof. Dr. Motomu Tanaka Lab)
2013-2016
PhD, Department of Chemistry, Saitama University
Japan Society for the Promotion of Science (JSPS) Research Fellow (doctor course), Department of Chemistry, Saitama University, Japan (Prof. Seiichiro Nakabayashi, Prof. Hiroshi Yoshikawa Lab)
2011-2013
M.S. Department of Chemistry, Saitama University
2007-2011
B.S. Department of Chemistry, Saitama University
Visualizing Soft Matter Interface
Interference reflection microscopy (IRM) has attracted attention because of its potential for nano-scale characterization of surface structures of biospecimen without fluorescence labeling. Using the interference between light reflected from the surface of the biospecimens (I1) and a substrate (I2), interference intensity (I) in monochromatic IRM images covnverted into height (h) at the resolution of the sub-nm scale in a label-free manner.With the aid of IRM, we have been used to reveal the adhesion fingerprint of cancer cells (Matsuzaki,...,Yoshikawa*, J. Phys Chem Letter 2014, J. Phys Chem B 2016, Bioconjugate Chemistry 2023*(corresponding), PNAS 2024), insect cells(Matsuzaki*,.., Yoshikawa: J. Phys Chem Lett. 2022). However, the lateral resolution of conventional IRM has been restricted to a diffraction limit that hinders the observation of nanometer- scale details in molecular organization and its dynamics of biological systems. To overcome long-standing limitations in lateral resolution for IRM, we have introduced 3-D structured illumination (3-D SIM) to IRM (Concept: Fig. 1left). We succeeded in visualizing surface nanostructures of protein crystal growth under lateral (dx-y ~ 110 nm) and axial (dx-z ≤ 5 nm) resolutions(Fig. 1bottom). Not 2-D, but 3-D structured illumination efficiently eliminated untargeted reflected light along an optical axis leading to visualize adhesion of microtubule fiber networks under a lateral resolution (dx-y ~ 120 nm) ten times greater than that of unstructured IRM (dx-y ~ 1000 nm) (Fig. 1 right). WIth the strong support of Prof. James K. Gimzewski, and Dr. Shivani Sharma, the work was published in J. Phys Chem Letter 2024. As a young PI, I will try to shed the light on the deeper insight into the surface nanostructure of soft matter.
Mechanobiology of Organoid
Since the discovery of regulating the differentiation of "single" stem cells by extracellular mechanics (Discher Cell 2006, Fig.2a), most researchers have relied on "single" cell-specific research (Weaver Nature Rev. Cancer 2009). Our collaborative trials were the first breakthrough to identify optimal mechanics for liver organogenesis (Takebe, .., Matsuzaki et al., Cell Stem Cell 2015, Fig.2b). Even though existing trends use hydrogels with "homogeneous" mechanics, I recently identified "heterogenous" mechanics in vivo (Fig.2c). My mechanically patterned hydrogel successfully mimics such environment controlling to the collective movement of cells. My work is a pioneer internationally in controlling various organ regeneration through designing "heterogeneous" mechanics (iScience 2022). As a young PI, "I" am promoting research that will open new fields in organ regeneration based on stiffness, such as liver (CellStemCell 2015, Stem Cell Reports 2018), bone (Stem Cell Res. Ther. 2022, iScience 2024.), colon (Mucosal Immunology 2023), muscle (iScience 2022).
