Original article
* Corresponding author
2026:
Md. Tanvir Islam, Hayato Takashima, Hiroyuki Yamakoshi, Hiroyuki Shibata, Wang Yinuo, Yoshiharu Iwabuchi, Takashi Toyama*, Yoshiro Saito*. Potent Nrf2 activation protects against ferroptosis despite impaired selenium metabolism by the monocarbonyl curcumin derivative GO-Y015. The Journal of Toxicological Sciences. In press.
Hiroyuki Yamakoshi, Hiro Ikeda, Wang Yinuo, Takashi Toyama, Hayato Takashima, Hideyuki Nagai, Ryo Watanabe, Yoshiro Saito, Yoshiharu Iwabuchi. Chemoselective Modification of Selenocysteine-Containing Biomolecules via Doubly Activated Cyclopropanes. ACS Omega. In press.
Hayato Takashima, Reiko Makino, Hiroki Taguchi, Yoshika Takenaka, Yasutoshi Akiyama, Yoshihisa Tomikoka, Daigo Sumi, Takashi Toyama*, Yoshiro Saito*. A novel ICP-MS strategy identifies arsenite as an inhibitor of selenocysteine-tRNASec charging. The Journal of Toxicological Sciences. Vol 51 (6), 331-338.
Satoru Shiina, Hayato Takashima, Hiroki Taguchi, Takayuki Kaneko, Kota Noritsugu, Takashi Toyama*, Yoshiro Saito*. Promotion of lead-induced cytotoxicity in differentiating neural cells and protective effects of selenium. Biological Trace Element Research. 1-14.
Hijiri Oshio, Isshin Shiiba, Naoki Ito, Fuya Yamaguchi, Naozumi Okada, Yuto Ishikawa, Shun Nagashima, Yuuta Fujikawa, Keitaro Umezawa, Yuri Miura, Misaki Shimizu, Yoshiro Saito, Tomoyuki Yamaguchi, Ryoko Inatome, and Shigeru Yanagi. Heme-derived iron Utilization in Mitochondria via the MITOL-HMOX2 Pathway at MERCs. Nature Cell Biology. 28, 1464-1479.
Wang Yinuo, Takashi Toyama*, Hiroyuki Yamakoshi, Hiroki Taguchi, Hayato Takashima, Ryo Watanabe, Yuichiro Mita, Junya Ito, Eikan Mishima, Yasutoshi Akiyama, Hiroyuki Shibata, Noriko Noguchi, Toshinari Takamura, Marcus Conrad, Yoshihisa Tomikoka, Yoshiharu Iwabuchi, Yoshiro Saito* Electrophilic monocarbonyl curcumin derivatives reveal differential vulnerabilities in the selenium metabolic network. Free Radical Biology and Medicine, Volume 253, Pages 487-499.
Hayato Takashima, Reiko Makino, Hiroki Taguchi, Yoshika Takenaka, Yasutoshi Akiyama, Yoshihisa Tomikoka, Daigo Sumi, Takashi Toyama* and Yoshiro Saito* A Novel ICP-MS Strategy Identifies Arsenite as an Inhibitor of Selenocysteine-tRNASec Charging. The Journal of Toxicological Sciences, Vol 51 (6), 331-338.
Md. Tanvir Islam, Hiroki Taguchi, Hiroyuki Yamakoshi, Wang Yinuo, Hiroyuki Shibata, Yoshiharu Iwabuchi, Takashi Toyama* and Yoshiro Saito* Nrf2 Activation by the Monocarbonyl Curcumin Derivative GO-Y015 Confers Cellular Protection Against Arsenite Toxicity by Reducing Intracellular Arsenic Levels. Scientific Reports, 25319.
Hayato Takashima, Reiko Makino, Hiroki Taguchi, Junya Ito, Eikan Mishima, Yoshika Takenaka, Yasutoshi Akiyama, Daigo Sumi, Marcus Conrad, Yoshihisa Tomikoka, Takashi Toyama*, Yoshiro Saito* Arsenite sensitizes to ferroptosis by disrupting selenium metabolism and reducing GPx4 expression. Toxicology, 154409.
2025:
Cynthia M Galicia-Medina, Hein Ko Oo, Takumi Nishiuchi, Ryota Tanida, Tuerdiguli Abuduyimiti, Hisanori Goto, Yujiro Nakano, Yumie Takeshita, Kiyo-aki Ishii, Takashi Toyama, Yoshiro Saito, Hiroaki Takayama, Toshinari Takamura. Cysteine redoxome landscape in the liver of male mice fed a high-fat high-sucrose diet. Journal of Biological Chemistry, 301, 10, 110730.
Yukina Iwamoto, Seiko Yamakoshi, Akiyo Sekimoto, Koji Hosomi, Takashi Toyama, Yoshiro Saito, Jun Kunisawa, Nobuyuki Takahashi, Eikan Mishima, Emiko Sato. Lactoferrin attenuates renal fibrosis and uremic sarcopenia in a mouse model of adenine-induced chronic kidney disease. The Journal of Nutritional Biochemistry, 146, 110039.
Kotoko Arisawa, Moeka Natori, Tetta Hiranuma, Misaki Shimizu, Yuto Yamazaki, Yasuhiro Miki, Takashi Toyama, Yoshiro Saito*. NRF2-dependent suppression of selenoprotein P expression promotes intracellular selenium metabolic remodeling and upregulation of antioxidant selenoproteins in hepatocellular carcinoma. Redox Biology, 86, 103821.
Yoshiro Saito*, Misaki Shimizu, Mitsuharu Sato, An Masuda, Kotoko Arisawa, Keiko Taguchi, Takashi Toyama, Ikuko N. Motoike, Kengo Kinoshita, Seizo Koshiba, Masayuki Yamamoto & Toshinari Takamura. Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study. Scientific Reports, 25319.
Atsuya Ichikawa, Takashi Toyama*, Hiroki Taguchi, Satoru Shiina, Hayato Takashima, Kazuaki Takahashi, Yasumitsu Ogra, Ayako Mizuno, Kotoko Arisawa, Yoshiro Saito*. The selenoprotein P/ApoER2 axis facilitates selenium accumulation in selenoprotein P-accepting cells and confers prolonged resistance to ferroptosis, Redox Biology, 83, 103664.
Hein Ko Oo, Cynthia M. Galicia-Medina, Takumi Nishiuchi, Ryota Tanida, Hisanori Goto, Yujiro Nakano, Yumie Takeshita, Yoshiro Saito, Hiroaki Takayama, Toshinari Takamura. Cysteine redoxome landscape in mouse brown adipose tissue under acute cold exposure, iScience, 28, 3, 112051.
Xinying Ye, *Takashi Toyama, Wang Yinuo, Runa Kudo, Siu Stephanie, Kotoko Arisawa, *Yoshiro Saito. Inhibition of selenium supply function of selenoprotein p through adduct formation by sulforaphane. The Journal of Nutritional Biochemistry, 135, 109781.
2024:
Mineshi Sakamoto, Masumi Marumoto, Koichi Haraguchi, Takashi Toyama, Yoshiro Saito, Steven J. Balogh, Chiharu Tohyama, and Masaaki Nakamura (2024) Assessing the role of selenium in Minamata disease through reanalysis of historical samples, Environmental International, 195(2025), 109242
Yusuke Hirata, Yuto Yamada, Soma Taguchi, Ryota Kojima, Haruka Masumoto, Shinnosuke Kimura, Takuya Niijima, Takashi Toyama, Ryoji Kise, Emiko Sato, Yasunori Uchida, Junya Ito, Kiyotaka Nakagawa, Tomohiko Taguchi, Asuka Inoue, Yoshiro Saito, Takuya Noguchi, Atsushi Matsuzawa. Conjugated fatty acids drive ferroptosis through chaperone-mediated autophagic degradation of GPX4 by targeting mitochondria. Cell Death & Disease, 84, 23.
Junya Ito, Toshitaka Nakamura, Takashi Toyama, Sebastian Doll, Mirai Suzuki, Weijia Zhang, Jiashuo Zheng, Dietrich Trümbach, Naoya Yamada, Koya Ono, Adam Wahida, Yoshiro Saito, Kiyotaka Nakagawa, Eikan Mishima, Marcus Conrad. PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization. Molecular Cell, 84, 23.
Hayato Takashima, *Takashi Toyama, Eikan Mishima, Kei Ishida, Yinuo Wang, Atsuya Ichikawa, Junya Ito, Syunsuke Yogiashi, Stephanie Siu, Mayumi Sugawara, Satoru Shiina, Kotoko Arisawa, Marcus Conrad, *Yoshiro Saito. Impact of selenium content in fetal bovine serum on ferroptosis susceptibility and selenoprotein expression in cultured cells. The Journal of Toxicological Sciences, 49,12,555-563.
Kohei Matsuda, Yuichiro Mita, Kazumasa Saigoh, Yoshiro Saito, and Noriko Noguchi (2024) Modifications of DJ-1 in which pI shifts to acidic in red blood cells have potential for biomarkers of Parkinson’s disease at early stages, Free Radical Research, 58, 748-757
Ryua Harima, Takahiro Sasaki, Takayuki Kaneko, Fuka Aso, Hayato Takashima, Takashi Toyama, Kenshiro Hara, Kentaro Tanemura, *Yoshiro Saito (2024) Ccdc152 is not necessary for male fertility, but contributes to maintaining sperm morphology, Journal of Reproduction and Development, 70, 396-404
Takashi Toyama*, Katsuki Sato, Yoshiro Saito*. Remodeling of Selenium Metabolism through Adduct Formation of Selenoprotein P with Epigallocatechin Gallate. Metallomics Research, 4, 3.
Christian Herder, Yoshiro Saito, Maria C Spagnuolo, Haifa Maalmi, Misaki Shimizu, Gidon J Bönhof, Keita Suzuki, Wolfgang Rathmann, Annette Peters, Michael Roden, Dan Ziegler, Barbara Thorand, Toshinari Takamura. Differential associations between selenoprotein P and distal sensorimotor polyneuropathy in people with and without diabetes: KORA F4/FF4 study. Free Radical Biology and Medicine, 223, 87-95.
Ryouhei Tsutsumi, Beatrix Ueberheide, Feng-Xia Liang, Benjamin G Neel, Ryuichi Sakai, Yoshiro Saito. Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation. Nature Communications, 15(1), 2843.
Xi Zheng, Takashi Toyama*, Stephanie Siu, Takayuki Kaneko, Hikari Sugiura, Shota Yamashita, Yoshiteru Shimoda, Masayuki Kanamori, Kotoko Arisawa, Hidenori Endo, Yoshiro Saito* (2024) Selenoprotein P expression in glioblastoma as a regulator of ferroptosis sensitivity: preservation of GPX4 via the cycling-selenium storage. Scientific Reports, 14 (1), 682
2023:
Xinying Ye, Takashi Toyama*, Keiko Taguchi, Kotoko Arisawa, Takayuki Kaneko, Ryouhei Tsutsumi, Masayuki Yamamoto, Yoshiro Saito* (2023) Sulforaphane decreases serum selenoprotein P levels through enhancement of lysosomal degradation independent of Nrf2. Commuications Biology, 6 (1), 2060
Ayako Mizuno, Takashi Toyama, Atsuya Ichikawa, Naoko Sakai, Yuya Yoshioka, Yukina Nishito, Renya Toga, Hiroshi Amesaka, Takayuki Kaneko, Kotoko Arisawa, Ryouhei Tsutsumi, Yuichiro Mita, Shun-Ichi Tanaka, Noriko Noguchi, Yoshiro Saito* (2023) An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase. Journal of Biological Chemistry, 299, 105009
Marco Vinceti, Teresa Urbano, Annalisa Chiari, Tommaso Filippini, Lauren A. Wise, Manuela Tondelli, Bernhard Michalke, Misaki Shimizu, Yoshiro Saito (2023) Selenoprotein P concentrations and risk of progression from mild cognitive impairment to dementia. Scientific Reports, 13, 8792
Takashi Toyama, Sidi Xu, Yoshitomi Kanemitsu, Takashi Hasegawa, Takuya Noguchi, Jin-Yong Lee, Atsushi Matsuzawa, Akira Naganuma, Gi-Wook Hwang (2023) Methylmercury directly modifies the 105th cysteine residue in oncostatin M to promote binding to tumor necrosis factor receptor 3 and inhibit cell growth. Archives of Toxicology, 97, 1887–1897
Kotoko Arisawa, Miyuki Kaneko, Ayumi Matsuoka, Natsuki Ozawa, Rie Kawawa, Tomoko Ishikawa, Ikuyo Ichi, Yoko Fujiwara (2023) Piceatannol Prevents Obesity and Fat Accumulation Caused by Estrogen Deficiency in Female Mice by Promoting Lipolysis. Nutrients, 15(6), 1374
Yusuke Hirata, Ruiqi Cai, Allen Volchuk, Benjamin E. Steinberg, Yoshiro Saito, Atsushi Matsuzawa, Sergio Grinstein, and Spencer A. Freeman (2023) Lipid peroxidation increases membrane tension, Piezo1 gating and cation permeability to execute ferroptosis. Current Biology, 33, 1282-1294
Takayuki Kaneko, Yuichiro Mita, Kanako Nozawa-Kumada, Masana Yazaki, Mieko Arisawa, Etsuo Niki, Noriko Noguchi, Yoshiro Saito* (2023) Antioxidant action of persulfides and polysulfides against free radical-mediated lipid peroxidation. Free Radical Research, 56 (9-10), 677-690
2022:
Kentaro Matsumoto, Shenwei Ni, Hiroyuki Arai, Takashi Toyama, Yoshiro Saito, Takehiro Suzuki, Naoshi Dohmae, Kojiro Mukai, Tomohiko Taguchi (2022) A non-nucleotide agonist that binds covalently to cysteine residues of STING. Cell Structure and Function, 48, 59-70
Yumie Takeshita, Takeo Tanaka, Hiroaki Takayama, Yuki Kita, Hisanori Goto, Yujiro Nakano, Yoshiro Saito, Toshinari Takamura (2022) Circulating selenoprotein P levels predict glucose-lowering and insulinotropic effects of metformin, but not alogliptin: A post-hoc analysis. Journal of Diabetes Investigation, 14, 230-235
Teresa Urbano, Marco Vinceti, Jessica Mandrioli, Annalisa Chiari, Tommaso Filippini, Roberta Bedin, Manuela Tondelli, Cecilia Simonini, Giovanna Zamboni, Misaki Shimizu, and Yoshiro Saito (2022) Selenoprotein P concentrations in cerebrospinal fluid and serum of individuals affected by amyotrophic lateral sclerosis, mild cognitive impairment and Alzheimer’s dementia. International Journal of Molecular Sciences, 23, 9865
Daichi Chida, Takashi Toyama*, Takanori Chiba, Takayuki Kaneko, Kotoko Arisawa and Yoshiro Saito* (2022) Effects of the Interplay between Selenocystine and Methylmercury on Their Cytotoxicity and Glucose-Driven Insulin Secretion from Mouse Insulinoma Cells. BPB Reports, 5, 74-79.
Swe Mar Oo, Hein Ko Oo, Hiroaki Takayama, Kiyo-aki Ishii, Yumie Takeshita, Hisanori Goto, Yujiro Nakano, Susumu Kohno, Chiaki Takahashi, Hiroyuki Nakamura, Yoshiro Saito, Mami Matsushita, Yuko Okamatsu-Ogura, Masayuki Saito, and Toshinari Takamura (2022) Selenoprotein P-mediated reductive stress impairs cold-induced thermogenesis in brown fat. Cell Reports, 38, 110566.
Machiko Honda, Tatsuya Segawa, Kiyoshi Ishikawa, Masahiro Maeda, Yoshiro Saito, and Shigeyuki Kon (2022) Nephronectin influences EAE development by regulating Th17/Treg balance via reactive oxygen species. American Journal of Physiology-Cell Physiology, 322(4), C699–C711.
Nanako Kitabayashi, Shohei Nakao, Yuichiro Mita, Kotoko Arisawa, Takayuki Hoshi, Takashi Toyama, Kiyo-aki Ishii, Toshinari Takamura, Noriko Noguchi, and Yoshiro Saito* (2022) Role of selenoprotein P expression in the function of pancreatic beta cells: Prevention of ferroptosis-like cell death and stress-induced nascent granule degradation. Free Radical Biology & Medicine, 183, 89-103.
2021:
Yuichiro Mita, Risa Uchida, Sayuri Yasuhara, Kohei Kishi, Takayuki Hoshi, Yoshitaka Matsuo, Tadashi Yokooji, Yoshino Shirakawa, Takashi Toyama, Yasuomi Urano, Toshifumi Inada, Noriko Noguchi and Yoshiro Saito* (2021) Identification of a novel endogenous long non-coding RNA that inhibits selenoprotein P translation. Nucleic Acids Research, 49 (12), 6893-6907.
Takashi Toyama, Takayuki Hoshi, Takuya Noguchi, Yoshiro Saito, Atsushi Matsuzawa, Akira Naganuma, Gi-Wook Hwang (2021) Methylmercury induces neuronal cell death by inducing TNF-α expression through the ASK1/p38 signaling pathway in microglia. Scientific Reports, 11, 9832
Hayato Irokawa, Satoshi Numasaki, Shin Kato, Kenta Iwai, Atsushi Inose-Maruyama, Takumi Ohdate, Gi-Wook Hwang, Takashi Toyama, Toshihiko Watanabe, Shusuke Kuge (2021) Comprehensive analyses of the cysteine thiol oxidation of PKM2 reveal the effects of multiple oxidation on cellular oxidative stress response. Biochemical Journal, 478 (7), 1453-1470.
Takashi Toyama, Yanjiao Wang, Min-Seok Kim, Tsutomu Takahashi, Akira Naganuma, Gi-Wook Hwang (2021) Increased expression of TCF3, transcription factor 3, is a defense response against methylmercury toxicity in mouse neuronal C17. 2 cells. Toxicological Research, 1-8.
Sekiguchi Yuto, Kudoh Yuki, Naganuma Rio, Kagi Tomohiro, Nishidate Akiko, Ishii Chizuru, Toyama Takashi, Hirata Yusuke, Hwang Gi-Wook, Matsuzawa Atsushi (2021) Gefitinib initiates sterile inflammation by promoting IL-1β and HMGB1 release via two distinct mechanisms. Cell Death and Disease, 12 (1).
2020:
Masato Kosuge, Emi Furusawa-Nishii, Koyu Ito, Yoshiro Saito, Kouetsu Ogasawara (2020) Point mutation bias in SARS-CoV-2 variants results in increased ability to stimulate inflammatory responses. Scientific Reports, 10, 17766
Naoki Endo, Takashi Toyama, Akira Naganuma, Yoshiro Saito, Gi-Wook Hwang (2020) Hydrogen Peroxide Causes Cell Death via Increased Transcription of HOXB13 in Human Lung Epithelial A549 Cells. Toxics, 8, 78
Yusuke Hirata, Yuki Nada, Yuto Yamada, Takashi Toyama, Kohji Fukunaga, Gi-Wook Hwang, Takuya Noguchi, Atsushi Matsuzawa (2020) Elaidic Acid Potentiates Extracellular ATP-Induced Apoptosis via the P2X7-ROS-ASK1-p38 Axis in Microglial Cell Lines. Biological and Pharmaceutical Bulletin, 43 (10), 1562-1569
Masayuki Sato, Takashi Toyama, Min-Seok Kim, Jin-Yong Lee, Takayuki Hoshi, Nobuhiko Miura, Akira Naganuma, Gi-Wook Hwang (2020) Increased putrescine levels due to ODC1 overexpression prevents mitochondrial dysfunction-related apoptosis induced by methylmercury. Life Sciences, 256, 118031
Mayuka Yamada, Midori Suzuki, Takuya Noguchi, Takumi Yokosawa, Yuto Sekiguchi, Natsumi Mutoh, Takashi Toyama, Yusuke Hirata, Gi-Wook Hwang, Atsushi Matsuzawa (2020) The Antibiotic Cefotaxime Works as Both an Activator of Nrf2 and an Inducer of HSP70 in Mammalian Cells. BPB Reports, 3 (1), 16-21
Takashi Toyama, Sidi Xu, Ryo Nakano, Takashi Hasegawa, Naoki Endo, Tsutomu Takahashi, Jin-Yong Lee, Akira Naganuma, Gi-Wook Hwang (2020) The Nuclear Protein HOXB13 Enhances Methylmercury Toxicity by Inducing Oncostatin M and Promoting Its Binding to TNFR3 in Cultured Cells. Cells, 9 (1), 45
Takeshi Niki, Jinro Endo, Kazuko Takahashi-Niki, Tatsuki Yasuda, Asami Okamoto, Yoshiro Saito, Hiroyoshi Ariga, Sanae M.M. Iguchi-Ariga (2020) DJ-1-binding compound B enhances Nrf2 activity through the PI3-kinase-Akt pathway by DJ-1-dependent inactivation of PTEN. Brain Research, 1729, 146641
2019:
Nobuhiro Kikuchi, Kimio Satoh, Taijyu Satoh, Nobuhiro Yaoita, Mohammad Abdul Hai Siddique, Junichi Omura, Ryo Kurosawa, Masamichi Nogi, Shinichiro Sunamura, Satoshi Miyata, Hirofumi Misu, Yoshiro Saito, Hiroaki Shimokawa (2019) Diagnostic and Prognostic Significance of Serum Levels of Selenoprotein P in Patients with Pulmonary Hypertension. Arteriosclerosis, Thrombosis, and Vascular Biology, 39, 2553-2562
Mayuka Kobayashi, Kana Muramatsu, Takamitsu Haruyama, Haruka Uesugi, Ai Kikuchi, Hiroki Konno, Noriko Noguchi, and Yoshiro Saito* (2019) Polymerization of oxidized DJ-1 via noncovalent and covalent binding: Significance of disulfide bond formation. ACS Omega, 4, 9603-14
Nobuhiro Kikuchi, Kimio Satoh, Yoshiro Saito, Hiroaki Shimokawa (2019) Response by Kikuchi et al Regarding Article, “Selenoprotein P Promotes the Development of Pulmonary Arterial Hypertension: A Possible Novel Therapeutic Target” Circulation, 139, 724-725
Kensuke Mohri, Hirofumi Misu, Hiroaki Takayama, Kiyo-aki Ishii, Akihiro Kikuchi, Fei Lan, Yasufumi Enyama, Yumie Takeshita, Yoshiro Saito, Shuichi Kaneko, and Toshinari Takamura (2019) Circulating concentrations of insulin resistance-associated hepatokines, selenoprotein P and leukocyte cell-derived chemotaxin 2, during an oral glucose tolerance test in humans. Biological and Pharmaceutical Bulletin, 42, 373-378
Takayuki Hoshi, Takashi Toyama, Youichi Shinozaki, Schuichi Koizumi, Jin-Yong Lee, Akira Naganuma, Gi-Wook Hwang (2019) Evaluation of M1-microglial
activation by neurotoxic metals using optimized organotypic cerebral slice cultures. The Journal of Toxicological Sciences, 44, 471-479.
Akiyuki Nishimura, Kakeru Shimoda, Tomohiro Tanaka, Takashi Toyama, Kazuhiro Nishiyama, Yasuhiro Shinkai, Takuro Numaga-Tomita, Daiju Yamazaki, Yasunari Kanda, Takaaki Akaike, Yoshito Kumagai, Motohiro Nishida. (2019) Depolysulfidation of Drp1 induced by low-dose methylmercury exposure increases cardiac
vulnerability to hemodynamic overload. Science Signaling, 12, 587
Min-Seok Kim, Tsutomu Takahashi, Jin-Yong Lee, Takashi Toyama, Takayuki Hoshi, Shusuke Kuge, Yasuyuki Fujiwara, Akira Naganuma, Gi-Wook Hwang (2019).
Methylmercury induces the expression of chemokine CCL4 via SRF activation in C17.2 mouse neural stem cells. Scientific Reports, 9.1.